Production of Organic Polymer Based Monolithic Columns for Anion Separation using High Performance Liquid Chromatography

Dewi Annisa’ (Jurusan Kimia Fakultas MIPA Universitas Brawijaya Malang)
Aprilia Nur Tasfiyati (Jurusan Kimia Fakultas MIPA Universitas Brawijaya Malang)
Hermin Sulistyarti (Jurusan Kimia Fakultas MIPA Universitas Brawijaya Malang)
Akhmad Sabarudin (Jurusan Kimia Fakultas MIPA Universitas Brawijaya Malang)

Natural B
Vol 3, No 1 (2015)

Methacrylate-based monolithic columns have been prepared in silanized silicosteel column (100 ´ 0.5 mm id) by in situ polymerization reaction, using glycidyl methacrylate (GMA) as monomer, ethylene dimethacrylate (EDMA) as crosslinker, porogen (1-propanol, 1,4 butandiol, water) and initiator AIBN.  Monolith poly-(GMA-co-EDMA) was then modified with diethylamine and benzyl chloride to obtain ammonium quartener as strong anion exchanger. From the characterization of 4 monolith in this study, monolithic columns poly-(GMA-co-EDMA) with %T 40, %C 25 and polymerization time 24 h has good mechanical stability and permeability, adequate molecular recognition sites (indicated by binding capacity value of 10,119 mg/mL), and has good proportion of flow throughpore and mesopores (45,04% and 41,32% respectively). It can successfully separate the mixture sample of anion consisting of fluoride, bromide, sulfate and nitrate ions by indirect UV detection.