Journal of Clinical Microbiology and Infectious Diseases
Vol. 2 No. 2 (2022): Available Online: December 2022

Challenges for Legionella pneumophila detection in Indonesia

Rivia Gina Rahmawaty (Department of Clinical Microbiology, Faculty of Medicine, Universitas Indonesia, dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia)
Mochamad Helmi Aziz (Department of Clinical Microbiology, Faculty of Medicine, Universitas Indonesia, dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia
Institute of Human Virology and Cancer Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia)

Chairinda Dachwan (Department of Clinical Microbiology, Faculty of Medicine, Universitas Indonesia, dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia)
Erie Yuwita Sari (Department of Clinical Microbiology, Faculty of Medicine, Universitas Indonesia, dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia)
Ibnu Agus Ariyanto (Institute of Human Virology and Cancer Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
UI Medical Laboratory, Jakarta, Indonesia)

Mardiastuti Wahid (Department of Clinical Microbiology, Faculty of Medicine, Universitas Indonesia, dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia)



Article Info

Publish Date
25 Dec 2022

Abstract

Legionella pneumophila (L. pneumophila) is widely known to cause respiratory illness outbreaks and remains underdiagnosed, including in Indonesia. Several diagnostic methods are available, yet none have been implemented as a routine diagnostic panel in most clinical microbiology laboratories in Indonesia. The urine antigen test is the cheapest and easy to perform. However, it only detects serogroup 1 of L. pneumophila, creating a blind spot for non-serogroup 1. Culture is the gold standard, but its sensitivity and turnaround time makes culture less feasible in a clinical setting. The direct fluorescent antibody is rapid, nonetheless, expertise and experience are needed to increase the sensitivity. Molecular methods, while very sensitive, cannot rule out contamination since the bacteria are mostly found in water. In order to validate which best method to be performed in Indonesia, a nationwide surveillance and validation study should be performed.

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Journal Info

Abbrev

JCMID

Publisher

Subject

Immunology & microbiology

Description

Journal of Clinical Microbiology and Infectious Diseases; peer-reviewed journal aiming to communicate high-quality research articles, reviews, and general articles in the field. JCMID publishes articles that encompass basic research/clinical studies related to microbiology and infectious disease. ...