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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Biotek Medisiana Indonesia Majalah Kedokteran Journal of Medicine and Health Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi Cakradonya Dental Journal Journal of Clinical Microbiology and Infectious Diseases Journal of Medicine and Health

Mardiastuti H Wahid

Departemen Mikrobiologi Klinik, Fakultas Kedokteran, Universitas Indonesia

Author-ID : 5908907

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Neuroscience Public Health

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Comparison of Studentsâ Characteristics, Self-Motivation, and Readiness of Self-Directed Learning Implementation among Medical Students at Maranatha Christian University Gunanegara, Rimonta F; Wahid, Mardiastuti H; Widyahening, Indah S
Journal of Medicine and Health Vol 1, No 5 (2017)
Publisher : Maranatha Christian University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Self-Directed Learning (SDL) is an important skill that must be achieved by medical students.The aim of this study is to identify the level of self-motivation and SDL readiness in the medicalstudents as well as to identify factors affecting SDL. This is is a mixed method research,involving first-year and clinical year medical students. A quantitative research is conducted bydistributing self-motivation (MSLQ) and SDL questionnaire (SDLRS). A total sampling isapplied to select the respondents. Furthermore, focus group discussion (FGD) on students andtutors/preceptors is carried out. Informants are chosen by purposive sampling method. Thisresearch reveals that most of medical students have a good level of self-motivation but a lowlevel of SDL readiness. Nevertheless, the mean scores of SDL readiness in both groups showsno significant differences. The research also identifies four major factors affecting the SDLreadiness, namely the studentsâ characteristics, learning process, the role of tutors/preceptorsand supporting facilities for learning. There is no significant difference between SDL readinessof the first-year and clinical year of medical students. Unprepared studentsâ characteristics,sub-optimal learning process, unsupported role of tutors/preceptors and inadequate learningresources are found to be the mayor factors influencing SDL readiness.Keywords: self-directed learning readiness; self-motivation; problem-based learning

Profil Candida penyebab kandidemia dan pola kepekaan terhadap anti jamur pada pasien sakit kritis di Rumah Sakit Cipto Mangunkusuno Mursinah, Mursinah; Ibrahim, Fera; Wahid, Mardiastuti
Jurnal Biotek Medisiana Indonesia Vol 5, No 2 (2016): :
Publisher : Puslitbang Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jbmi.v5i2.7706.105-111

Candida spesies is important cause of nosocomial infection that lead to death and prolonged hospital stay. Guideline management of candidiasis by Infectious Diseases Society of America (IDSA) in 2009 proposed early antifungal therapy in critical patient with the risk of invasive candidiasis. Early identification ofÂ sepsis patien with high risk of fungal infection is challenging becauseÂ long and low result from blood culture. Antifungal usage without appropriate indication can cause resistance to antifungal. The aim of the study is obtain data candida species that caused candidemia in Cipto Mangunkusuno hospital (RSCM) and sensitivity pattern to antifungal. This retrospective study used patient medical record from 2011-2014. This study had 117 candidemia case in RSCM during 2011-2014. Every year candidemia was dominated by CandidaÂ tropicalis, CandidaÂ albicans, and CandidaÂ parapsilosis with trend the increase number of CandidaÂ tropicalis since 2013. Susceptibility pattern of Candida that cause candidemia in RSCM from 71 isolat tested the result was86% spesies Candida flukonazol sensitive, 99% vorikonazol sensitive, 97% amfoterisin B sensitive and 100% flusitosin sensitive.

Lactobacillus plantarum PADA FESES INDIVIDU DEWASA SEHAT YANG MENGONSUMSI Lactobacillus plantarum IS-10506 DARI DADIH [Lactobacillus plantarum in Stool of Apparently Healthy Adults Consuming Lactobacillus plantarum IS-10506 from Dadih] Azmier Adib; Mardiastuti H Wahid; Pratiwi Sudarmono; Ingrid S. Surono
Jurnal Teknologi dan Industri Pangan Vol. 24 No. 2 (2013): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

A placebo double blind pre-post human study was conducted in apparently healthy adults. There were two treatment groups consisting of Group A and B representing probiotic and placebo group, respectively. Twenty four participants were randomly assigned, each supplemented with either placebo or probiotic Lactobacillus plantarum IS-10506. The micro encapsulated powder was given at a dose of 2.6x1010 CFU/day for 21 consecutive days. Stool samples were collected before and after the supplementation. The fresh stool samples were analyzed for the viability of Lactobacillus sp. by conventional plate count method in MRS agar. Some stool samples were kept frozen to be analyzed by using real time PCR to trace back the availability of Lactobacillus plantarum with species specific primer. The Lactobacillus sp. in stools of healthy adults given microencapsulated probiotic Lactobacillus plantarum IS-10506 powder was significantly more than those who consumed microencapsulated placebo powder. Molecular detection by qPCR confirmed the availability of Lactobacillus plantarum in fecal samples of the probiotic group after given the supplementation for 21 days. The molecular detection validation confirmed that probiotic Lactobacillus plantarum was available in the fecal samples of the probiotic group of healthy adults. However, the availability and viability of Lactobacillus plantarum were not consistently found in the intestinal tract.

Tips Merancang Pemicu dalam Problem-Based Learning Mardiastuti H. Wahid
Majalah Kedokteran UKI Vol. 30 No. 1 (2014): JANUARI - MARET
Publisher : Fakultas Kedokteran Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/mkvol34iss2pp60

Abstrak Problem-based learning (PBL) adalah metode belajar bagi mahasiswa dengan pendekatan pembelajaran pilihan yang menunjang prinsip student-centered, active learning. Prinsip dasar PBL adalah sekelompok mahasiswa menentukan kebutuhan belajarnya setelah mendisuksikan satu masalah/pemicu. Terdapat tiga elemen penting dalam PBL yaitu pemicu, fasilitator, dan diskusi kelompok mahasiswa. Keberhasilan implementasi PBL tergantung dari kualitas disain pemicu. Pemicu atau masalah dapat berupa deskripsi alamiah tentang suatu fenomena seperti fenomena fisiologis, biologis, psikologis, dan sosial. Pemicu seyogyanya disusun berdasarkan pengetahuan yang telah dimiliki mahasiswa sebelumnya (prior knowledge) dan derajat kompleksitas sesuai dengan tingkat pendidikan mahasiswa. Ada beberapa tipe pemicu antara lain explanation problem, discussion problem, strategy problem, application problem, dan multilevel problem. Dalam PBL, mahasiswa belajar keterampilan dan sikap yang sesuai dengan praktik kedokteran dan kehidupan mahasiswa. Termasuk di dalamnya cara berkomunikasi dengan sejawat dan orang awam; bekerja dalam tim, membangun inisiatif, berbagi informasi dan menghargai sesama. Selama proses penyusunan, pemicu dikaji secara berkesinambungan sebelum akhirnya disampaikan ke mahasiswa dan fasilitator. Setelah pemicu digunakan, langkah selanjutnya adalah umpan balik dari mahasiswa dan fasilitator.Kata kunci: problem-based learning, student-centered, active learning, kurikulum berbasis kompetensi, pemicu Abstract Problem-based learning (PBL) is a method of learning for students as a choosen learning approach that supports the principle of student-centered active learning. The basic principle of PBL is that a group of students decide their own learning needs after discussing a problem/trigger. There are three key elements in PBL: trigger, facilitator, and group discussion. The efficacy of PBL implementation depends on the trigger design quality. Trigger can be a natural description of a physiological, biological, psychological or social phenomenon. Trigger should be arranged based on students’ prior knowledge and degree of complexity according to their level of education. There are several types of trigger, i.e explanation problem, discussion problem, strategy problem, application problem and multilevel problem. In PBL, students learn skills and behaviours according to medical practice and their daily lives. The skills and behaviours include how to communicate with colleagues and laymen, work in a team, build initiative, share information and appreciate others. During the arrangement process, trigger is assessed continualy before it is finally delivered to students and facilitators. After the trigger is used, the next step is a feedback from students and facilitators. Keywords: Problem-based learning, student-centered, active learning, competency-based curriculum, trigger

Comparison of Studentsâ€™ Characteristics, Self-Motivation, and Readiness of Self-Directed Learning Implementation among Medical Students at Maranatha Christian University Rimonta F Gunanegara; Mardiastuti H Wahid; Indah S Widyahening
Journal of Medicine and Health Vol. 1 No. 5 (2017)
Publisher : Universitas Kristen Maranatha

Self-Directed Learning (SDL) is an important skill that must be achieved by medical students.The aim of this study is to identify the level of self-motivation and SDL readiness in the medicalstudents as well as to identify factors affecting SDL. This is is a mixed method research,involving first-year and clinical year medical students. A quantitative research is conducted bydistributing self-motivation (MSLQ) and SDL questionnaire (SDLRS). A total sampling isapplied to select the respondents. Furthermore, focus group discussion (FGD) on students andtutors/preceptors is carried out. Informants are chosen by purposive sampling method. Thisresearch reveals that most of medical students have a good level of self-motivation but a lowlevel of SDL readiness. Nevertheless, the mean scores of SDL readiness in both groups showsno significant differences. The research also identifies four major factors affecting the SDLreadiness, namely the studentsâ€™ characteristics, learning process, the role of tutors/preceptorsand supporting facilities for learning. There is no significant difference between SDL readinessof the first-year and clinical year of medical students. Unprepared studentsâ€™ characteristics,sub-optimal learning process, unsupported role of tutors/preceptors and inadequate learningresources are found to be the mayor factors influencing SDL readiness.Keywords: self-directed learning readiness; self-motivation; problem-based learning

CORRELATION BETWEEN MEDICAL STUDENTS’ PERCEPTION ON LEARNING ENVIRONMENT AND STRESS LEVEL Resti Rahmadika Akbar; Mardiastuti Wahid; Retno Asti Werdhani
Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Vol 8, No 1 (2019): Maret
Publisher : Asosiasi Institusi Pendidikan Kedokteran Indonesia

Background: Learning environment in medical education is one of several aspect determine students’ academic success. The medical education itself has been the biggest source of depression or stress for students, beside personal, financial, or family problems. The purpose of this study is to assess the correlation between students’ perception on learning environment and stress levels.Methods: This study was a cross sectional study, conducted from December 2016 to April 2017, involving the 1st, 2nd, 3rd, 4th year students of the Faculty of Medicine, Baiturrahmah University (FK UNBRAH), Padang, with a total of 595 students. Students’ perceptions on learning environment were assessed using the Dundee Ready Educational Environment Measure (DREEM) questionnaire and the student stress level was assessed by the questionnaire of Depression Anxiety Stress Scale 42 (DASS 42). Both questionnaires have been validated and available in Bahasa.Results: Respondents involved in this study were 477 (80.1%).The median of the students’s perceptions on their learning environment was 132 (92-200), which means "more positive than negative". Students’ perceptions on learning environment between 1st year students with other academic years differed significantly. There was no significant difference between female and male students' perceptions on the learning environment. The median value of student stress level of FK UNBRAH was categorized as normal. There was no statistically significant difference in stress level based on academic level and gender. The correlation between students’ perception toward learning environment and academic level was found to be significant with very weak negative correlation (p<0,05)Conclusion: The better students’ perception on the learning environment, the lower the stress level.

HUBUNGAN ANTARA SELF-EFFICACY TERHADAP PRESTASI AKADEMIK PADA MAHASISWA KEDOKTERAN GIGI (TINJAUAN PUSTAKA) Nurul Husna; Luthfi Saiful Arif; Mardiastuti Wahid
Cakradonya Dental Journal Vol 14, No 2 (2022): Agustus 2022
Publisher : FKG Unsyiah

Self-efficacy merupakan salah satu bagian dari pilar utama dalam social cognitive theory (SCT) yang dikembangkan oleh Albert Bandura. Berbagai studi melaporkan bahwa self-efficacy berkaitan dengan prestasi akademik mahasiswa. Tinjauan pustaka ini membahas konsep self-efficacy mulai dari definisi, kategori, aspek, peran, faktor yang mempengaruhi, hubungan antara self-efficacy dengan prestasi akademik dan alat ukur yang digunakan untuk menilai self-efficacy pada mahasiswa kedokteran gigi. Kata kunci yang digunakan dalam pencarian meliputi ‘self-efficacy’, ‘academic performance’, ‘dental student’ and ‘dentistry’ pada database NCBI, Sage Publication, Science Direct, PubMed, Google Scholar dan sumber terpercaya lainnya. Dari hasil penelurusan didapatkan bahwa hanya satu literatur dari Kolombia yang membahas hubungan self-efficacy dengan academic performance pada mahasiswa kedokteran gigi. Telusur literatur menunjukkan beberapa alat ukur yang dapat digunakan untuk menilai self-efficacy, yaitu General Self-Efficacy Scale, The Collage Academic Self-Efficacy Scale, The Academic Self-Efficacy, dan Collage Self-Efficacy Inventory. Alat ukur ini menjadi tolak ukur untuk mengetahui tinggi atau rendahnya self-efficacy seseorang. Mahasiswa dengan self-efficacy tinggi memiliki kemampuan dalam mengatur, melaksanakan, dan menyelesaikan masalah yang berkaitan dengan tugas belajar, serta berkeyakinan dapat menyelesaikan tugas dengan baik. Pengetahuan tentang tingkat self-efficacy mahasiswa dapat dijadikan sebagai prediktor terhadap keberhasilan akademik mahasiswa tersebut.

Leptospirosis Ditinjau dari Aspek Mikrobiologi Ika Ningsih; Mardiastuti H Wahid
EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi, dan Mikrobiologi Vol 7 No 1 (2022): Ekotonia: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi
Publisher : Department of Biology, Faculty of Agriculture, Fisheries and Biology, University of Bangka Belitung

Leptospirosis is an acute infectious disease in humans caused by Leptospira sp. This disease is classified as a zoonosis that usually occurs during the flood and is commonly transmitted through rat urine. Leptospirosis occurs through humans contact with animals or the environment that have been contaminated by Leptospira. There are wide spectrum clinical manifestations of leptospirosis varying from self-limited to severe disease. Leptospira commonly enters human body through conjunctiva or injured skin, food or beverage contaminated with urine’s rat containing Leptospira. This disease is very difficult to be distinguished from other diseases such as meningitis, malaria, dengue fever, hepatitis and enteric fever. Clinical symptoms of leptospirosis are nonspecific and due to the difficulties in conforming the diagnosis so that resulted to the misdiagnosis of this disease. Microbiological examination is done by Rapid Diagnostic Test, Microscopic Agglutination Test, Polymerase Chain Reaction test and others. Prevention can be done by inhibiting the entry of Leptospira to human body via implementing clean and healthy life and surrounding environment.

The current trend for prosthetic joint infection diagnosis from culture to molecular: a literature review Augustine Natasha; Mardiastuti Wahid; Pratiwi Sudarmono
Journal of Clinical Microbiology and Infectious Diseases Vol. 1 No. 1 (2021): Available Online: June 2021
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v1i1.6

Pathogen identification in prosthetic joint infection is necessary to achieve optimal patient management. The specimens for diagnosis of prosthetic joint infection could be the synovial fluid, the tissue obtained intraoperatively, and the biofilm from the implanted prosthesis. Because of the low sensitivity of the conventional specimen culture method, the preanalytic treatment of the specimen was widely studied to increase the yield of detection. This review aimed to describe the current specimen processing methods used in the clinical setting to increase the pathogen detection rate. A blood culture bottle, tissue homogenization, and explanted prosthesis sonication were the most studied methods with a good result. Molecular methods were also developed to reduce the time of pathogen detection. MALDI-TOF was studied to reduce identification time after a positive culture. Other molecular methods such as polymerase chain reaction and next-generation sequencing were studied to omit the culture step and reduce detection time. However, the impracticality and the inconsistent sensitivity of certain specimens from the molecular methods limit its application in the clinical setting. Specimen culture remains as a crucial step in the current prosthetic joint infection, with the improvement of the molecular methods toward a better prosthetic joint infection diagnosis.

Challenges for Legionella pneumophila detection in Indonesia Rivia Gina Rahmawaty; Mochamad Helmi Aziz; Chairinda Dachwan; Erie Yuwita Sari; Ibnu Agus Ariyanto; Mardiastuti Wahid
Journal of Clinical Microbiology and Infectious Diseases Vol. 2 No. 2 (2022): Available Online: December 2022
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v2i2.35

Legionella pneumophila (L. pneumophila) is widely known to cause respiratory illness outbreaks and remains underdiagnosed, including in Indonesia. Several diagnostic methods are available, yet none have been implemented as a routine diagnostic panel in most clinical microbiology laboratories in Indonesia. The urine antigen test is the cheapest and easy to perform. However, it only detects serogroup 1 of L. pneumophila, creating a blind spot for non-serogroup 1. Culture is the gold standard, but its sensitivity and turnaround time makes culture less feasible in a clinical setting. The direct fluorescent antibody is rapid, nonetheless, expertise and experience are needed to increase the sensitivity. Molecular methods, while very sensitive, cannot rule out contamination since the bacteria are mostly found in water. In order to validate which best method to be performed in Indonesia, a nationwide surveillance and validation study should be performed.

Co-Authors Akbar, Resti Rahmadika Andi Yasmon Angga W Lokeswara, Angga W Ariyani Kiranasari Augustine Natasha Aziz, Mochamad Helmi Azmier Adib Azmier Adib Chairinda Dachwan Conny Tjampakasari Effendi, Evita Erie Yuwita Sari Fathan, Hafizah Fera Ibrahim Ibnu Agus Ariyanto Ika Ningsih Ika Ningsih Indah S. Widyahening Ingrid S. Surono Luthfi Saiful Arif Mochamad Helmi Aziz Mursinah Mursinah Mursinah, Mursinah Ni Made Adi Tarini Nurul Husna Oliver E Yausep, Oliver E Pratiwi Pujilestari Sudarmono Raksheeth Agarwal, Raksheeth Retno Asti Werdhani Retno Kadarsih Rimonta F Gunanegara Rimonta F Gunanegara, Rimonta F Rivia Gina Rahmawaty Samsuridjal Djauzi Sari, Meutia Kumala Sharon Hanmy Angel, Sharon Hanmy Sitohang, Irma Bernadette Simbolon Yeva Rosana

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