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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Biotek Medisiana Indonesia Medical Journal of Indonesia Majalah Kedokteran Journal of Medicine and Health Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi Cakradonya Dental Journal Journal of Clinical Microbiology and Infectious Diseases Journal of Medicine and Health
Mardiastuti H Wahid
Departemen Mikrobiologi Klinik, Fakultas Kedokteran, Universitas Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Neuroscience Public Health

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Patterns of bacterial resistance against Ceftriaxone from 2002 to 2005 in the Clinical Microbiology Laboratory of the Faculty of Medicine, University of Indonesia Rosana, Yeva; Kiranasari, Ariyani; Ningsih, Ika; Tjampakasari, Conny; Kadarsih, Retno; Wahid, Mardiastuti H.
Medical Journal of Indonesia Vol 16, No 1 (2007): January-March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.758 KB) | DOI: 10.13181/mji.v16i1.248

Abstract

The spread of drug resistant microbes is a global public health challenge which impairs the efficacy of antimicrobial agents and causes substantial increase in morbidity and mortality rates, including healthcare-associated costs. Monitoring of antimicrobial drug resistance from documented microbial epidemiology & resistance rate is useful in preventing the emergence of resistance. This study reports on the pattern of bacterial resistance against ceftriaxone in the past 4 years. The data were obtained from specimens examined in the Clinical Microbiology Laboratory, Department of Microbiology Faculty of Medicine, University of Indonesia from 2002 to 2005. Microbial species were determined from culture and identification tests. Disc diffusion method was used for sensitivity testing of ceftriaxone to 14 Gram-negative and 7 Gram-positive bacteria. Although resistance rates were increased from 2002 to 2005, resistance rates of ceftriaxone were found to be less than 50%. Low resistance rates (< 3%) were observed for Salmonella typhi, Salmonella paratyphi A, Shigella flexneri, Serratia marcescens, and Streptococcus pneumoniae. These results could be useful in developing guidelines on the use of ceftriaxone in Indonesia. (Med J Indones 2007; 16:3-6) Keywords: Microbial drug resistance, disc diffusion method, Gram-positive, Gram-negative
Resistance patterns of microbes isolated from gastrointestinal tract Wahid, Mardiastuti H.; Ningsih, Ika; Adib, Azmier
Medical Journal of Indonesia Vol 20, No 2 (2011): May
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (37.388 KB) | DOI: 10.13181/mji.v20i2.437

Abstract

Background: Digestive tract infection with clinical manifestation of diarrhea is an infectious disease that has the highest morbidity and mortality rate, especially in developing countries. Diarrhea causes mortality mostly in infants under one year old. Improvement in management is done continuously, but advances in diagnosis and therapy cannot be reached by developing countries. One of the etiological agents causing infection of digestive tract is bacteria. Therefore, knowledge of bacteria that cause gastrointestinal infection and their resistance patterns may support the management of this disease. The aim of this study was to examine microbes that were isolated from the digestive tract and their resistance patterns against antibiotics.Methods: Samples (stool, rectal/anal swab) were collected from the Clinical Microbiology Laboratory, FKUI during 2005-2008. Isolation, identifi cation and sensitivity test were conducted according to standard laboratory procedures. Interpretation of sensitivity test was done according to NCCLS/CLSI guidance. Data was analyzed using WHOnet version 5.3.Results: We found 28 isolates of pathogenic Escherichia coli, 1 isolate of S. paratyphi A and 4 isolates of yeasts. Pathogenic Escherichia coli were still sensitive against some antibiotics, but the sensitivity was reduced against amoxicillin, sulbenicillin, ticarcillin and trimethoprim/sulfamethoxazole.Conclusion: The most predominant gastrointestinal tract infection causing microbes was pathogenic Escherichia coli. These bacteria showed decrease sensitivity against some antibiotics commonly used to treat patients with gastrointestinal tract infection. (Med J Indones 2011; 20:105-8)Keywords: antibiotics, Escherichia coli, gastrointestinal infection
Development of multiplex-PCR assay for rapid detection of Candida spp. Tarini, Ni Made A.; Wahid, Mardiastuti H.; Ibrahim, Fera; Yasmon, Andi; Djauzi, Samsuridjal
Medical Journal of Indonesia Vol 19, No 2 (2010): May
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (108.411 KB) | DOI: 10.13181/mji.v19i2.387

Abstract

Aim Candida spp. infection commonly occur in immunocompromised patients. Biochemical assay for identification of Candida spp. is time-consuming and shows many undetermined results. Specific detection for antibody, antigen and metabolites of Candida spp. had low sensitivity and specificity. In this study, we developed a rapid diagnostic method, Multiplex-PCR, to identify Candida spp.Methods Five Candida spp. isolates were cultured, identifi ed with germ tube and API® 20 C AUX (BioMerieux® SA) kit. Furthermore, DNA was purified by QIAamp DNA mini (Qiagen®) kit for Multiplex-PCR assay.Results DNA detection limit by Multiplex-PCR assays for C. albicans, C. tropicalis, C. parapsilosis, C. krusei and C. glabrata were 4 pg, 0.98 pg, 0.98 pg, 0.5 pg and 16 pg respectively. This assay was also more sensitive than culture in that Multiplex-PCR could detect 2.6-2.9 x 100 CFU/ml, whereas culture 2.6-2.9 x 102 CFU/ml.Conclusion Multiplex-PCR is much more sensitive than culture and thus, can be recommended as a sensitive and specific assay for identification of Candida spp. (Med J Indones 2010; 19:83-7)Keywords: Candida spp., multiplex-PCR
Comparison of Students’ Characteristics, Self-Motivation, and Readiness of Self-Directed Learning Implementation among Medical Students at Maranatha Christian University Gunanegara, Rimonta F; Wahid, Mardiastuti H; Widyahening, Indah S
Journal of Medicine and Health Vol 1, No 5 (2017)
Publisher : Maranatha Christian University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Self-Directed Learning (SDL) is an important skill that must be achieved by medical students.The aim of this study is to identify the level of self-motivation and SDL readiness in the medicalstudents as well as to identify factors affecting SDL. This is is a mixed method research,involving first-year and clinical year medical students. A quantitative research is conducted bydistributing self-motivation (MSLQ) and SDL questionnaire (SDLRS). A total sampling isapplied to select the respondents. Furthermore, focus group discussion (FGD) on students andtutors/preceptors is carried out. Informants are chosen by purposive sampling method. Thisresearch reveals that most of medical students have a good level of self-motivation but a lowlevel of SDL readiness. Nevertheless, the mean scores of SDL readiness in both groups showsno significant differences. The research also identifies four major factors affecting the SDLreadiness, namely the students’ characteristics, learning process, the role of tutors/preceptorsand supporting facilities for learning. There is no significant difference between SDL readinessof the first-year and clinical year of medical students. Unprepared students’ characteristics,sub-optimal learning process, unsupported role of tutors/preceptors and inadequate learningresources are found to be the mayor factors influencing SDL readiness.Keywords: self-directed learning readiness; self-motivation; problem-based learning
Profil Candida penyebab kandidemia dan pola kepekaan terhadap anti jamur pada pasien sakit kritis di Rumah Sakit Cipto Mangunkusuno Mursinah, Mursinah; Ibrahim, Fera; Wahid, Mardiastuti
Jurnal Biotek Medisiana Indonesia Vol 5, No 2 (2016): :
Publisher : Puslitbang Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jbmi.v5i2.7706.105-111

Abstract

Candida spesies is important cause of nosocomial infection that lead to death and prolonged hospital stay. Guideline management of candidiasis by Infectious Diseases Society of America (IDSA) in 2009 proposed early antifungal therapy in critical patient with the risk of invasive candidiasis. Early identification of  sepsis patien with high risk of fungal infection is challenging because  long and low result from blood culture. Antifungal usage without appropriate indication can cause resistance to antifungal. The aim of the study is obtain data candida species that caused candidemia in Cipto Mangunkusuno hospital (RSCM) and sensitivity pattern to antifungal. This retrospective study used patient medical record from 2011-2014. This study had 117 candidemia case in RSCM during 2011-2014. Every year candidemia was dominated by Candida  tropicalis, Candida  albicans, and Candida  parapsilosis with trend the increase number of Candida  tropicalis since 2013. Susceptibility pattern of Candida that cause candidemia in RSCM from 71 isolat tested the result was86% spesies Candida flukonazol sensitive, 99% vorikonazol sensitive, 97% amfoterisin B sensitive and 100% flusitosin sensitive.
The susceptibility of pathogens associated with acne vulgaris to antibiotics Sitohang, Irma Bernadette Simbolon; Fathan, Hafizah; Effendi, Evita; Wahid, Mardiastuti
Medical Journal of Indonesia Vol 28, No 1 (2019): March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (327.641 KB) | DOI: 10.13181/mji.v28i1.2735

Abstract

BACKGROUND Acne vulgaris is a pilosebaceous disorder. Bacterial activity and inflammation both influence acne formation. Antibiotics suppress the bacterial activities and elicit anti-inflammatory effects. The overuse of antibiotics may lead to resistance in bacteria. This study was aimed to provide an overview of bacteria that may cause acne and determine their susceptibility to antibiotics.METHODS This was a cross-sectional study sampling from 93 patients with acne in Ciptomangunkusumo Hospital. Comedones were extracted and cultured on Brucella blood agar, under aerobic and anaerobic conditions at 35oC for 24–48 hours. Bacterial identification was performed using Vitek®, and susceptibility test using E-test. Data interpretation was based on the Clinical and Laboratory Standards Institute 2015.RESULTS Staphylococcus epidermidis (50.5%), Propionibacterium acnes (11.0%), and Staphylococcus aureus (7.7%) were identified. Bacteria were not found in 69.2% and 1.1% of samples in anaerobic and aerobic cultures, respectively. P. acnes was susceptible to doxycycline (100%) and minocycline (100%), while 10% was resistant to erythromycin, clindamycin, and tetracycline. S. epidermidis was susceptible to minocycline (100%); but resistant to erythromycin (65.2%), clindamycin (52.2%) and tetracycline (32.6%). The susceptibility of S. epidermidis to doxycycline was 89.1%, which was lower than that of P. acnes (100%). S. aureus was found to be sensitive to minocycline (100%), doxycycline (71.4%), clindamycin (71.4%), and tetracycline (71.4%); but it was resistant to erythromycin (42.9%).CONCLUSIONS Doxycycline and minocycline showed 100% effectiveness for P. acnes. The isolated bacteria were more susceptible to doxycycline compared to tetracycline. The use of clindamycin and erythromycin needs to be limited as most S. epidermidis isolates were resistant to both.
Lactobacillus plantarum PADA FESES INDIVIDU DEWASA SEHAT YANG MENGONSUMSI Lactobacillus plantarum IS-10506 DARI DADIH [Lactobacillus plantarum in Stool of Apparently Healthy Adults Consuming Lactobacillus plantarum IS-10506 from Dadih] Azmier Adib; Mardiastuti H Wahid; Pratiwi Sudarmono; Ingrid S. Surono
Jurnal Teknologi dan Industri Pangan Vol. 24 No. 2 (2013): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (539.775 KB) | DOI: 10.6066/jtip.2013.24.2.154

Abstract

A placebo double blind pre-post human study was conducted in apparently healthy adults. There were two treatment groups consisting of Group A and B representing probiotic and placebo group, respectively. Twenty four participants were randomly assigned, each supplemented with either placebo or probiotic Lactobacillus plantarum IS-10506. The micro encapsulated powder was given at a dose of 2.6x1010 CFU/day for 21 consecutive days. Stool samples were collected before and after the supplementation. The fresh stool samples were analyzed for the viability of Lactobacillus sp. by conventional plate count method in MRS agar. Some stool samples were kept frozen to be analyzed by using real time PCR to trace back the availability of Lactobacillus plantarum with species specific primer. The Lactobacillus sp. in stools of healthy adults given microencapsulated probiotic Lactobacillus plantarum IS-10506 powder was significantly more than those who consumed microencapsulated placebo powder. Molecular detection by qPCR confirmed the availability of Lactobacillus plantarum in fecal samples of the probiotic group after given the supplementation for 21 days. The molecular detection validation confirmed that probiotic Lactobacillus plantarum was available in the fecal samples of the probiotic group of healthy adults. However, the availability and viability of Lactobacillus plantarum were not consistently found in the intestinal tract.
Tips Merancang Pemicu dalam Problem-Based Learning Mardiastuti H. Wahid
Majalah Kedokteran UKI Vol. 30 No. 1 (2014): JANUARI - MARET
Publisher : Fakultas Kedokteran Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/mkvol34iss2pp60

Abstract

Abstrak Problem-based learning (PBL) adalah metode belajar bagi mahasiswa dengan pendekatan pembelajaran pilihan yang menunjang prinsip student-centered, active learning. Prinsip dasar PBL adalah sekelompok mahasiswa menentukan kebutuhan belajarnya setelah mendisuksikan satu masalah/pemicu. Terdapat tiga elemen penting dalam PBL yaitu pemicu, fasilitator, dan diskusi kelompok mahasiswa. Keberhasilan implementasi PBL tergantung dari kualitas disain pemicu. Pemicu atau masalah dapat berupa deskripsi alamiah tentang suatu fenomena seperti fenomena fisiologis, biologis, psikologis, dan sosial. Pemicu seyogyanya disusun berdasarkan pengetahuan yang telah dimiliki mahasiswa sebelumnya (prior knowledge) dan derajat kompleksitas sesuai dengan tingkat pendidikan mahasiswa. Ada beberapa tipe pemicu antara lain explanation problem, discussion problem, strategy problem, application problem, dan multilevel problem. Dalam PBL, mahasiswa belajar keterampilan dan sikap yang sesuai dengan praktik kedokteran dan kehidupan mahasiswa. Termasuk di dalamnya cara berkomunikasi dengan sejawat dan orang awam; bekerja dalam tim, membangun inisiatif, berbagi informasi dan menghargai sesama. Selama proses penyusunan, pemicu dikaji secara berkesinambungan sebelum akhirnya disampaikan ke mahasiswa dan fasilitator. Setelah pemicu digunakan, langkah selanjutnya adalah umpan balik dari mahasiswa dan fasilitator.Kata kunci: problem-based learning, student-centered, active learning, kurikulum berbasis kompetensi, pemicu Abstract Problem-based learning (PBL) is a method of learning for students as a choosen learning approach that supports the principle of student-centered active learning. The basic principle of PBL is that a group of students decide their own learning needs after discussing a problem/trigger. There are three key elements in PBL: trigger, facilitator, and group discussion. The efficacy of PBL implementation depends on the trigger design quality. Trigger can be a natural description of a physiological, biological, psychological or social phenomenon. Trigger should be arranged based on students’ prior knowledge and degree of complexity according to their level of education. There are several types of trigger, i.e explanation problem, discussion problem, strategy problem, application problem and multilevel problem. In PBL, students learn skills and behaviours according to medical practice and their daily lives. The skills and behaviours include how to communicate with colleagues and laymen, work in a team, build initiative, share information and appreciate others. During the arrangement process, trigger is assessed continualy before it is finally delivered to students and facilitators. After the trigger is used, the next step is a feedback from students and facilitators. Keywords: Problem-based learning, student-centered, active learning, competency-based curriculum, trigger
Comparison of Students’ Characteristics, Self-Motivation, and Readiness of Self-Directed Learning Implementation among Medical Students at Maranatha Christian University Rimonta F Gunanegara; Mardiastuti H Wahid; Indah S Widyahening
Journal of Medicine and Health Vol. 1 No. 5 (2017)
Publisher : Universitas Kristen Maranatha

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (598.99 KB) | DOI: 10.28932/jmh.v1i5.541

Abstract

Self-Directed Learning (SDL) is an important skill that must be achieved by medical students.The aim of this study is to identify the level of self-motivation and SDL readiness in the medicalstudents as well as to identify factors affecting SDL. This is is a mixed method research,involving first-year and clinical year medical students. A quantitative research is conducted bydistributing self-motivation (MSLQ) and SDL questionnaire (SDLRS). A total sampling isapplied to select the respondents. Furthermore, focus group discussion (FGD) on students andtutors/preceptors is carried out. Informants are chosen by purposive sampling method. Thisresearch reveals that most of medical students have a good level of self-motivation but a lowlevel of SDL readiness. Nevertheless, the mean scores of SDL readiness in both groups showsno significant differences. The research also identifies four major factors affecting the SDLreadiness, namely the students’ characteristics, learning process, the role of tutors/preceptorsand supporting facilities for learning. There is no significant difference between SDL readinessof the first-year and clinical year of medical students. Unprepared students’ characteristics,sub-optimal learning process, unsupported role of tutors/preceptors and inadequate learningresources are found to be the mayor factors influencing SDL readiness.Keywords: self-directed learning readiness; self-motivation; problem-based learning
CORRELATION BETWEEN MEDICAL STUDENTS’ PERCEPTION ON LEARNING ENVIRONMENT AND STRESS LEVEL Resti Rahmadika Akbar; Mardiastuti Wahid; Retno Asti Werdhani
Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Vol 8, No 1 (2019): Maret
Publisher : Asosiasi Institusi Pendidikan Kedokteran Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1614.827 KB) | DOI: 10.22146/jpki.44857

Abstract

Background: Learning environment in medical education is one of several aspect determine students’ academic success. The medical education itself has been the biggest source of depression or stress for students, beside personal, financial, or family problems. The purpose of this study is to assess the correlation between students’ perception on learning environment and stress levels.Methods: This study was a cross sectional study, conducted from December 2016 to April 2017, involving the 1st, 2nd, 3rd, 4th year students of the Faculty of Medicine, Baiturrahmah University (FK UNBRAH), Padang, with a total of 595 students. Students’ perceptions on learning environment were assessed using the Dundee Ready Educational Environment Measure (DREEM) questionnaire and the student stress level was assessed by the questionnaire of Depression Anxiety Stress Scale 42 (DASS 42). Both questionnaires have been validated and available in Bahasa.Results: Respondents involved in this study were 477 (80.1%).The median of the students’s perceptions on their learning environment was 132 (92-200), which means "more positive than negative". Students’ perceptions on learning environment between 1st year students with other academic years differed significantly. There was no significant difference between female and male students' perceptions on the learning environment. The median value of student stress level of FK UNBRAH was categorized as normal. There was no statistically significant difference in stress level based on academic level and gender. The correlation between students’ perception toward learning environment and academic level was found to be significant with very weak negative correlation (p<0,05)Conclusion: The better students’ perception on the learning environment, the lower the stress level.  
Co-Authors Akbar, Resti Rahmadika Andi Yasmon Angga W Lokeswara, Angga W Ariyani Kiranasari Augustine Natasha Aziz, Mochamad Helmi Azmier Adib Azmier Adib Chairinda Dachwan Conny Tjampakasari Effendi, Evita Erie Yuwita Sari Fathan, Hafizah Fera Ibrahim Ibnu Agus Ariyanto Ika Ningsih Ika Ningsih Indah S. Widyahening Ingrid S. Surono Luthfi Saiful Arif Mochamad Helmi Aziz Mursinah Mursinah Mursinah, Mursinah Ni Made Adi Tarini Nurul Husna Oliver E Yausep, Oliver E Pratiwi Pujilestari Sudarmono Raksheeth Agarwal, Raksheeth Retno Asti Werdhani Retno Kadarsih Rimonta F Gunanegara Rimonta F Gunanegara, Rimonta F Rivia Gina Rahmawaty Samsuridjal Djauzi Sari, Meutia Kumala Sharon Hanmy Angel, Sharon Hanmy Sitohang, Irma Bernadette Simbolon Yeva Rosana