Availability of meat and egg among rural communities comes from native chickens. However, the productivity of native chickens is still relatively low. The aim of this research was to determine the effect of in ovo feed of glutamine on hatchability and hatching weight of native chicken egg. As many 300 fertile eggs used, which were injected with glutamine at day 7 of incubation period. The research was arranged as a Completely Randomized Design (CRD) of 5 treatments, 3 replications. As for the treatments consisted of P0 (negative control); P1 (injected 0,5 ml 0,9% NaCl); P2 (injected 0,5 ml 0,5% glutamine in 0,9% NaCl); P3 (injected 0,5 ml 1,0% glutamine in 0,9% NaCl); P4 (injected 0,5 ml 1,5% glutamine in 0,9% NaCl). The results indicated that hatchability of the eggs fed in ovo by 0,9% NaCl (P1) and 0,5% glutamine (P2) were higher compared to the control and the other treatments. Thus, in ovo feed of glutamine on the 7thday of incubation resulted in higher hatchability with the injection of NaCl without glutamine and the hatching weight tended to bee higher at the level of 1,5%.
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