This study aims to determine the water quality of the Mako-Mako River based on physical, chemical, and microorganism parameters, as well as the effect of metal and microorganism levels on the color of the water and sediment. Water, sediment, and plankton samples were collected using purposive sampling at three locations: upstream, middle, and downstream. The concentrations of Fe and Mn in water and sediment were analyzed using AAS, while plankton identification was performed using a binocular microscope. The results of the water quality analysis of the Mako-Mako River show that the parameters of temperature, TDS, TSS, and Mn are still below the water quality standard for class 1, except for the Fe and total coliform parameters, whose concentrations have exceeded the standard. The metal content of Fe in the sediment is high and affects the color of the Mako-Mako River sediment, which ranges from reddish-brown to dark brown (deep). The presence of plankton, with a low abundance and diversity index, did not affect the color of the river water or the sediment of the Mako-Mako River. Therefore, a processing treatment is needed to use the Mako-Mako River water as a source of clean and raw water.
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