Jurnal Laboratorium Khatulistiwa
Vol 1, No 1 (2017): November 2017

Pengaruh Lamanya Penyimpanan Serum pada Suhu 20-80 0c Selama Satu Minggu terhadap Kadar Kolesterol Total

Laila Kamila (Jurusan Analis Kesehatan, Poltekkes Kemenkes Pontianak)
Slamet Slamet (Jurusan Analis Kesehatan, Poltekkes Kemenkes Pontianak)



Article Info

Publish Date
30 Nov 2017

Abstract

Abstract: Laboratory examinations are essential to establish diseases diagnosis so the results should be accurate. Therefore, it is necessary to control the pre-analytic, analytic and post-analytic. Cholesterol examination is one of the ways to establish the diagnosis of DM, Heart and hypertension. This study was aimed to determine the effect of duration of serum storage at a temperature of 2°-8°C for a week with total cholesterol levels. Samples were checked daily for a week. The samples of this study were the 2nd and 3rd grade of Health Analyst’s students who lived in the male dormitory where total samples were 5 samples. The study was conducted from March to June 2013. Each sample was checked for total serum cholesterol levels which stored at a temperature of 2°-8°C for a week. This examination used CHOD-PAP method. From the results of data analysis with Friedman Test method, it was determined that total cholesterol levels in serum which stored at temperature 2°-8°C for a week obtained Chi-Square value = 28.827 and p = 0.000 where showed that there was effect of duration of serum storage at temperature 2°-8°C for a week with total cholesterol level.Abstrak: Pemeriksaan laboratorium sangat penting untuk menegakkan diagnosa penyakit sehingga hasil harus akurat. Oleh karena itu, perlu dilakukan pengendalian terhadap pra-analitik, analitik dan pasca analitik. Pemeriksaan kolesterol merupakan salah satu upaya untuk menegakkan diagnosa penyakit DM, Jantung, dan hipertensi. Penelitian ini bertujuan untuk mengetahui pengaruh lamanya penyimpanan serum pada suhu 2°- 8°C selama 1 minggu terhadap kadar kolesterol total. Sampel diperiksa setiap hari selama 1 minggu. Sampel penelitian ini adalah mahasiswa tingkat 2 dan 3 Analis Kesehatan yang tinggal di asrama putra dengan jumlah sampel sebanyak 5 sampel. Penelitian ini dilaksanakan pada bulan Maret-Juni 2013. Masing-masing sampel diperiksa kadar kolesterol total pada serum yang disimpan pada suhu 2°- 8°C selama 1 minggu. Pemeriksaan ini menggunakan metode CHOD-PAP. Dari hasil analisis data dengan metode Friedman Test, diketahui bahwa kadar kolesterol total pada serum yang disimpan pada suhu 2°- 8°C selama 1 minggu didapatkan besaran nilai Chi Square = 28,827 dan p = 0.000. Hasil uji signifkansi Chi Square metode Friedman test menunjukkan bahwa p < 0.05 sehingga dapat disimpulkan bahwa terdapat pengaruh lamanya penyimpanan pada serum yang disimpan pada suhu 2°- 8°C selama 1 minggu terhadap kadar kolesterol total.

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Journal Info

Abbrev

JLK

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Description

The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have been achieved in the area of Medical Laboratory. Jurnal Laboratorium Khatulistiwa particularly focuses on the main problems in the development of the Medical Laboratory health ...