Indonesian Journal of Biotechnology
Vol 29, No 1 (2024)

Easy extraction of Ganoderma boninense liquid sample using portable on‐chip device

Adella Josephin (Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Depok 16424, West Java, Indonesia
Research Center of Biomedical Engineering, Universitas Indonesia, Depok 16424, West Java, Indonesia)

Yudan Whulanza (Department of Mechanical Engineering, Faculty of Engineering, Universitas Indonesia, Depok 16424, West Java, Indonesia
Research Center of Biomedical Engineering, Universitas Indonesia, Depok 16424, West Java, Indonesia)

Siti Fauziyah Rahman (Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Depok 16424, West Java, Indonesia
Research Center of Biomedical Engineering, Universitas Indonesia, Depok 16424, West Java, Indonesia)

Kenny Lischer (Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok 16424, West Java, Indonesia
Research Center of Biomedical Engineering, Universitas Indonesia, Depok 16424, West Java, Indonesia)

Muhammad Imam Surya (Research Center for Applied Botany, National Research and Innovation Agency, Cibinong, Bogor 16911, West Java, Indonesia)
Irfan Martiansyah (Research Center for Applied Botany, National Research and Innovation Agency, Cibinong, Bogor 16911, West Java, Indonesia)
Wiguna Rahman (Research Center for Applied Botany, National Research and Innovation Agency, Cibinong, Bogor 16911, West Java, Indonesia)
Uda Hashim (Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Seriab 01000 Kangar, Perlis, Malaysia)



Article Info

Publish Date
30 Mar 2024

Abstract

Detecting Ganoderma boninense in Indonesia is crucial for effectively controlling and mitigating the spread of basal stem disease in oil palm fields. While there is ongoing development of tolerant plants, no such plant has been successfully created yet. Consequently, researchers are actively studying detection methods for Ganoderma boninense. One established and highly accurate approach is the use of polymerase chain reaction (PCR) techniques for molecular detection. However, this method requires time‐consuming sample preparation, which can pose challenges in plantation settings. To address this problem, a portable lab‐on‐chip device has been introduced. This technology enables easy and automatic DNA retrieval from liquid samples by absorbing lysed DNA using magnetic beads. An efficient mechanism for manipulating the magnetic bead within the semiconductor has been successfully implemented. The extraction process typically takes around 15 minutes using a modified methodology on the chip device approach. The chip facilitates the retrieval of two samples with a capacity of 120 µL for each sample. The PCR method was utilized to validate the equivalence of the lab‐on‐chip device extraction to the standard extraction method. This represents a promising alternative for expedited and simplified detection of Ganoderma boninense in field conditions.

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Journal Info

Abbrev

ijbiotech

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology

Description

The Indonesian Journal of Biotechnology (IJBiotech) is an open access, peer-reviewed, multidisciplinary journal dedicated to the publication of novel research in all aspects of biotechnology, with particular attention paid to the exploration and development of natural products derived from ...