Uji Fenotipik Deteksi Enzim ESBL, AmpC dan Karbapenemase pada Bakteri Enterobacteriaceae

Ade Dharmawan (Departemen Mikrobiologi, Fakultas Kedokteran, Universitas Kristen Krida Wacana)
Nicolas Layanto (Departemen Mikrobiologi, Fakultas Kedokteran, Universitas Kristen Krida Wacana)

Jurnal Kedokteran Meditek
Vol 25 No 3 (2019): SEPTEMBER - DESEMBER

Organisms that express extended-spectrum β-lactamase (ESBLs) such as Enterobacteriaceae have been increasing for the past forty years. Organisms that express this enzyme can hydrolyze oxyminocephalosporins and monobactam,but can be inhibited using β-lactam inhibitors. There are various types of enzymes that contribute to cephalosporin and monobactam resistances, such as AmpC β-lactamase, and carbapenemase enzymes. Polymerase Chain Reaction (PCR) is an accurate method for detecting ESBL, but this method is a genotypic method that requires a specialized instrument. Therefore there is a need for a phenotypic method to detect ESBL, AmpC and Carbapenemase that can be conducted in various laboratories.