Simultaneous Analysis of Dopamine and Ascorbic Acid Using Polymelamine/Gold Nanoparticle-Modified Carbon Paste Electrode

Muji Harsini (Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Campus C, Mulyorejo, Surabaya 60115, Indonesia Chemosensor and Biosensor Research Group, Universitas Airlangga, Campus C, Mulyorejo, Surabaya 60115, Indonesia)
Ainy Nur Farida (Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Campus C, Mulyorejo, Surabaya 60115, Indonesia)
Erna Fitriany (Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Campus C, Mulyorejo, Surabaya 60115, Indonesia Akademi Farmasi Mitra Sehat Mandiri, Jl. Ki Hajar Dewantara No. 200, Ngingas, Krian, Sidoarjo, East Java 61262, Indones)
Denok Risky Ayu Paramita (Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Campus C, Mulyorejo, Surabaya 60115, Indonesia Jember Pharmacy Academy, Jl. Pangandaran 42, Antirogo, Sumbersari, Jember 68125, Indonesia)
Afaf Baktir (Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Campus C, Mulyorejo, Surabaya 60115, Indonesia)
Fredy Kurniawan (Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Jl. Raya ITS, Keputih, Sukolilo, Surabaya 60111, Indonesia)
Satya Candra Wibawa Sakti (Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Campus C, Mulyorejo, Surabaya 60115, Indonesia Supramodification Nano-Micro Engineering Research Group, Universitas Airlangga, Campus C, Mulyorejo, Surabaya 60115, In)
Yudhi Dwi Kurniawan (Research Center for Pharmaceutical Ingredient and Traditional Medicine, National Research and Innovation Agency, Jl. Raya Bogor Km 46, Cibinong 16911, Indonesia)
Bernadeta Ayu Widyaningrum (Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Campus C, Mulyorejo, Surabaya 60115, Indonesia Research Center for Biomass and Bioproducts, National Research, and Innovation Agency, Jl. Raya Bogor Km 46, Cibinong 1)

Indonesian Journal of Chemistry
Vol 24, No 1 (2024)

Modification of electrode using polymelamine (PM) and gold nanoparticles (AuNPs) has been successfully developed via electropolymerization and electrodeposition onto carbon paste electrode (CPE) using cyclic voltammetry (CV) technique. The modified electrode (AuNPs/PM/CPE) was applied as voltammetry sensors in a simultaneous of dopamine (DA) and ascorbic acid (AA). AuNPs/PM/CPE presented an effective surface area 5 times wider than CPE and demonstrated good electrocatalytic performance in the oxidation of DA and AA in 0.1 M phosphate buffer solution (pH 3) with a scan rate of 100 mV s−1. The differential pulse voltammetry (DPV) technique was chosen as the best method for separating potential peaks of DA and AA. The linear response for determining DA and AA using the DPV technique produced a concentration range of 0.1–13 and 0.4–12 µM with coefficient linearity of 0.9999 and 0.9997, the limit of detection of 0.1405 and 0.2187 µM, the accuracy of 89.62–109.16%, and 83.63–105.08%, and the precision of 0.017–0.701% and 0.066–0.626%, respectively. In addition, this electrode was applied in a real sample of infant urine with a concentration of 1 µM by spike method and found 98.86 and 98.28% as percent recovery of DA and AA, respectively.