Kultivasi
Vol 22, No 3 (2023): Jurnal Kultivasi

Callus induction and proliferation of Centella asiatica L. generated from leaves and petioles in the presence of Dicamba and BAP

Rahayu, Suci (Unknown)
Saptadi, Darmawan (Unknown)
Azmi, Chotimatul (Unknown)
Kusumanegara, Kusumawaty (Unknown)
Handayani, Tri (Unknown)
Roostika, Ika (Unknown)
Bermawie, Nurliani (Unknown)
Maulana, Haris (Unknown)



Article Info

Publish Date
30 Dec 2023

Abstract

Centella's need for industrial raw materials is high as a medicinal plant. These needs can be met through rapid multiplication using tissue culture techniques. In this study, induction and proliferation for a callus of centella cv. Castina 3 was conducted in the MS basal medium plus 4 mgL-1 Dicamba withand enriched with 7 concentrations of BAP (0, 0.1, 0.3, 0.5, 0.7, 0.9, and 1.1 mgL-1). Two kinds of explant were used, i.e., leaf and petiole. The results revealed that the addition of BAP in MS plus Dicamba medim stimulated better and produced a higher callus growth rate, both from leaf and petiole explants, than that media with Dicamba alone. Furthermore, 4 mgL-1 Dicamba + 1.1 mgL-1 BAP had a friable callus in the induction phase and a friable-compact callus in the proliferation phase. From this finding, it can be considered to use a combination of 4 mgL-1 Dicamba with 1.1 mgL-1 BAP in callus induction and proliferation for Centella rapid multiplication. 

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Journal Info

Abbrev

Kultivasi

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Jurnal Kultivasi diterbitkan oleh Departemen Budidaya Pertanian, Fakultas Pertanian, Universitas Padjadjaran. Jurnal ini terbit tiga kali dalam setahun, yaitu pada bulan Maret, Agustus, dan Desember. Kultivasi mempublikasikan hasil penelitian dan pemaparan ilmiah dari para dosen dan peneliti di ...