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All Journal Jurnal Agro Kultivasi
Chotimatul Azmi, Chotimatul
Balai Penelitian Tanaman Sayuran
Agriculture, Biological Sciences & Forestry

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Callus induction and proliferation of Centella asiatica L. generated from leaves and petioles in the presence of Dicamba and BAP Rahayu, Suci; Saptadi, Darmawan; Azmi, Chotimatul; Kusumanegara, Kusumawaty; Handayani, Tri; Roostika, Ika; Bermawie, Nurliani; Maulana, Haris
Kultivasi Vol 22, No 3 (2023): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v22i3.50581

Abstract

Centella's need for industrial raw materials is high as a medicinal plant. These needs can be met through rapid multiplication using tissue culture techniques. In this study, induction and proliferation for a callus of centella cv. Castina 3 was conducted in the MS basal medium plus 4 mgL-1 Dicamba withand enriched with 7 concentrations of BAP (0, 0.1, 0.3, 0.5, 0.7, 0.9, and 1.1 mgL-1). Two kinds of explant were used, i.e., leaf and petiole. The results revealed that the addition of BAP in MS plus Dicamba medim stimulated better and produced a higher callus growth rate, both from leaf and petiole explants, than that media with Dicamba alone. Furthermore, 4 mgL-1 Dicamba + 1.1 mgL-1 BAP had a friable callus in the induction phase and a friable-compact callus in the proliferation phase. From this finding, it can be considered to use a combination of 4 mgL-1 Dicamba with 1.1 mgL-1 BAP in callus induction and proliferation for Centella rapid multiplication. 
Yield components and seed attribute of nine varieties of open-pollinated chili. Azmi, Chotimatul; Sembiring, Asma; Kurniawan, Helmi
Jurnal AGRO Vol 11, No 1 (2024)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/23133

Abstract

Many studies related to chili varieties and productivity have been conducted. However, studies on the potential of seed production based on seed rendements to fulfil the need for chilli seeds are still limited. The study aimed to present rendement results, yield, and quality of the nine open-pollinated chilli seed varieties owned by the Indonesian Vegetable Research Institute (IVegRI), i.e. were Tanjung-2, Lembang 1, Ciko, Lingga, Kencana, Prima Agrihorti, Rabani Agrihorti, Carvi Agrihorti, and Branang, planted in a screen house. This study was conducted from January to December 2019. Cultivation techniques and seed certification followed the applicable Procedures. Data were analysed using descriptive methods. Observations were made on harvested fruits for yield and quality of seeds produced. The study revealed that Branang had the highest chili productivity (34.15 gm-2). Meanwhile, the highest seed rendement was Lembang 1 (5.48%). All varieties had good germination, namely above 75%. Based on the results, the breeders are expected to obtain information related to seed productivity from chili varieties owned by the Indonesian Vegetable Crops Research Institute (IVegRI). Penelitian terkait varietas dan produktivitas cabai telah banyak dilakukan. Akan tetapi, penelitian tentang potensi produksi benih berdasarkan rendemen benih untuk memenuhi kebutuhan benih cabai masih terbatas. Penelitian ini bertujuan untuk menyajikan hasil rendemen, daya hasil, dan mutu sembilan varietas benih cabai menyerbuk terbuka milik Balai Penelitian Tanaman Sayuran (Balitsa) yaitu Tanjung-2, Lembang 1, Ciko, Lingga, Kencana, Prima Agrihorti, Rabani Agrihorti, Carvi Agrihorti, dan Branang yang ditanam di rumah kaca. Penelitian ini dilaksanakan pada bulan Januari sampai dengan Desember 2019. Teknik budidaya dan sertifikasi benih mengikuti Prosedur yang berlaku. Data dianalisis dengan metode deskriptif. Pengamatan dilakukan terhadap buah yang dipanen untuk mengetahui daya hasil dan mutu benih yang dihasilkan. Hasil penelitian menunjukkan bahwa Branang memiliki produktivitas cabai tertinggi (34,15 gm-2). Sementara itu, rendemen benih tertinggi adalah Lembang 1 (5,48%). Semua varietas memiliki daya berkecambah yang baik, yaitu di atas 75%. Berdasarkan hasil penelitian ini diharapkan para pemulia tanaman dapat memperoleh informasi terkait produktivitas benih dari varietas cabai yang dimiliki Balai Penelitian Tanaman Sayuran (Balitsa).
Co-Authors Asma Sembiring Darmawan Saptadi, Darmawan Helmi Kurniawan Ika Roostika Kusumanegara, Kusumawaty Maulana, Haris Nurliani Bermawie Suci Rahayu Tri Handayani