Biomedical Engineering
Vol 1, No 1 (2015): BIOMEDICAL ENGINEERING

Phytochemical assay, potential of antimalarial and antioxidant activities of green tea extract and its fraction

Fanny Rahardja (Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia)



Article Info

Publish Date
15 Aug 2015

Abstract

Objective : The research objective was to evaluate the phytochemical compounds, the antioxidant and anti-malarial activity of green tea extract and its fraction. Methods : The antioxidant activity were measured by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity used in vitro laboratories prospective experimental design and completely randomized design, using ethanol extract, hexane, ethyl acetate, butanol fraction, and water fraction of green tea in 7 levels of concentration compared to epicatechin (EC). The data were analyzed using linear regression analysis, and were continued by determined Inhibitory Concentration50 (IC50). The anti-malarial activity of green tea fractions were compared to artemisinin. The data were analyzed using probit analysis, and were continued by determined IC50 .Result: The results showed that the highest antioxidant activity (IC50) was the fraction of ethyl acetate 2,184 µg/ml. The anti-malarial activity IC50of water and ethyl acetate fraction, ethanol extract and butanol fraction were 0,000090 µg/mL,0,003 µg/mL, 0,006 µg/mL, 0,011 µg/ml respectively.Conclusion : Extract and fractions of green tea have potential anti-malarial and antioxidant properties.

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