Ekstraksi DNA Phytophthora palmivora Isolat Kelapa

Achmadi Priyatmojo (Fakultas Pertanian Universitas Gadjah Mada)
Siti Subandiyah (Fakultas Pertanian Universitas Gadjah Mada)

Jurnal Perlindungan Tanaman Indonesia
Vol 2, No 1 (1996)

Coconut isolate of Phytophthora palmivora DNA was extracted from sporangium and mycelium using SDS and Proteinase-K method. Sporangium and mycelium were harvested from liquid and solid culture of Potato Dextrose medium. The result of the study showed that the DNA extracted from sporangium and mycelium of 8 days solid culture was seen as distinct band. On the other hand, the dNA from sporangium and mycelium of 24 days old liquid culture was fragmented with smear band appearance.