Effect of pentagamavunon-O on rat kidney’s glutathione s-transferase in-vivo with 1-chloro-2, 4-dinitro benzene as a substrate

Rohman, Abdul (Department of Chemical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia.)
Martono, Sudibyo (Department of Chemical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia.)
A. Margono, Supardjan (Department of Chemical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia.)

INDONESIAN JOURNAL OF PHARMACY
Vol 15 No 1, 2004

Glutathione S-transferase (GST) is a detoxifying enzyme which plays an important role in glutathione conjugation reaction with electrophilic xenobiotic on phase two metabolism. In this research, the effect of oral administration of PGV-0 on rat kidney’s GST activity was investigated.To represent GST activity isolated from rat kidney, 1-chloro-2,4-dinitro benzen (CDNB) was used as a substrate on conjugation with glutathione (GSH) spectrophotometrically. The activity of rat kidney’s GSTs from untreated rat was used as control, Based on data, it can be concluded that PGV-0 showed an inhibitory effect on rat kidney’s GSTs. The optimum effect was obtained at 40 mg/kgBW with % inhibition value of 16,16.Key words : Pentagamavunon-0, Glutathione S-transferase, 1-chloro-2,4-dinitro benzene