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All Journal Journal of Food and Pharmaceutical Science Jurnal Ilmiah Farmasi Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Dentika Dental Journal Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Cancer Chemoprevention The Indonesian Journal of Dental Research Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Jurnal Farmasi Sains dan Komunitas PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) JFIOnline Media Farmasi Indonesia Jurnal Kefarmasian Indonesia
Sudibyo Martono
Faculty Of Pharmacy, Universitas Gadjah Mada Yogyakarta, Indonesia
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Other

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Effect of nonsteroidal antiinflammatory agent on Sprague-Dawley rats liver glutathione S-transferases activity Sudibyo Martono, Sudibyo Martono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 37, No 03 (2005)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Glutathion S-transferase (GST) is a group of multifunction isoenzymes playing a role as katalisator in releasing inflammatory mediators prostaglandin and leukotrien. Non-steroidal antiinflammatory drugs (NSAID) such as pentagamavunon-0 (Pgv-0), piroxicam, and mefenamic acid have antiinflammatory effect. Aim of study: The study was to know the effect of pentagamavunon-0 (Pgv-0), piroxicam and mefenamic acid on GST.Materials and methods: The effects of non-steroidal antiinflammatory drugs (NSAID.) piroxicam, mefenamic acid, and pentagamavunon-0 (Pgv-O) in vitro on mu and pi classes of Sprague-Dawley rats liver glutathione S-transferases (GST)-activity was studied. GST-activities was measured spectrophotometrically on the conjugation reaction between glutathione (GSH) and 1,2-dichloro-4-nitrobenzene (for representing the mu class of GST) or etachrynic acid (for representing the pi class of GST). The potency of inhibitory effect was stated as IC50 value (the concentration of inhibitor resulting in 50 % inhibition of GST-activity).Results: The result showed that piroxicam inhibited strongly the mu class of GST-activity with IC60 value of 42.66 mM and inhibited weakly the pi class of GST-activity with IC50 value of 100.79 mM. Mefenamic acid inhibited weakly the mu and pi classes of GST-activity with IC50 values of 107.09 and 275,0 mM (extrapolated), respectively. In this research, Pgv-0 was found to be the strongest inhibitor of the mu class of GST-activity with IC50 value of 3.91 mM and did not inhibit the pi class of rats liver GST-activity.Conclusions: Based on the results, it can be concluded that the three NSAIDs studied, all showed the inhibitory effects of mu class of GST-activity in decreasing order of Pgv-0, piroxicam, and mefenamic acid. Three of them did not or very weakly inhibited the pi class of rats liver GST-activity.Key words: NSAIDs - glutathione S-transferase - pentagamavunon-0 - 1,2-dikloro-4-nitrobenzene - etachrynic acid. 
OPTIMASI FORMULASI SIRUP FRAKSI TIDAK LARUT ETIL ASETAT YANG MENGANDUNG ALKALOID DARI BUNGA KEMBANG SEPATU (Hibiscus rosa-sinensis L.) Murrukmihadi, Mimiek; Wahyuono, Subagus; Marchaban, Marchaban; Martono, Sudibyo
Majalah Obat Tradisional Vol 16, No 2 (2011)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.064 KB) | DOI: 10.14499/mot-TradMedJ16iss2pp101-108

Abstract

Fraksi tidak larut etilasetat yang mengandung alkaloid dari bunga kembang sepatu (Hibiscus rosa-sinensis L.) dengan kadar 0,6% b/v mempunyai aktivitas mukolitik yang setara dengan asetilsistein 0,1% berdasarkan kapasitas menurunkan viskositas mukus. Penggunaan bunga kembang sepatu sebagai obat batuk baru dilakukan secara tradisional. Oleh karena itu dibuat sediaan sirup fraksi tidak larut etilasetat yang mengandung alkaloid dari bunga kembang sepatu. Penelitian ini bertujuan untuk mengetahui formula optimum sirup fraksi etanolik yang mengandung alkaloid dari bunga kembang sepatu menggunakan metode Simplex Lattice Design. Fraksi tidak larut etilasetat yang mengandung alkaloid dari bunga kembang sepatu diperoleh dengan menggunakan Vacuum Liquid Chromatography (VLC). Metode Simplex Lattice Design digunakan untuk optimasi formula sirup fraksi tidak larut etilasetat yang mengandung alkaloid dari bunga kembang sepatu  dengan tujuh formula berdasarkan variasi jumlah gliserin, larutan sorbitol 70%, dan mucilago CMC-Na 0,5%. Sifat fisik sirup diuji untuk mendapatkan nilai respon total (R total) terbesar sebagai parameter formula optimum menggunakan metode Simplex Lattice Design  dengan  software Design Expert® versi 8.0.2. Sirup formula optimum diperoleh dengan proporsi gliserin sebesar 25,376%; larutan sorbitol 70% sebesar 51,985%; dan mucilago CMC-Na 0,5% sebesar 22,639%. Sirup formula optimum dibuat, kemudian diuji sifat fisik selama 4 minggu penyimpanan. Data yang diperoleh dari uji sifat fisik sirup dibandingkan dengan nilai prediksi dengan software Design Expert® versi 8.0.2. Stabilitas fisik sirup formula optimum minggu ke 0 dibandingkan terhadap stabilitas fisik  minggu ke 4 menggunakan uji-t berpasangan. Hasil menunjukkan bahwa sifat fisik formula optimum sirup fraksi tidak larut etilasetat yang mengandung alkaloid dari bunga kembang sepatu tidak berbeda signifikan dengan prediksi untuk kemudahan dituang dan tanggap rasa kecuali  viskositas dan derajat keasaman. Sirup fraksi etilasetat yang mengandung alkaloid  dari bunga kembang sepatu hasil optimasi kurang stabil selama empat minggu penyimpanan.
PENETAPAN KADAR ALKALOID DARI EKSTRAK ETANOLIK BUNGA KEMBANG SEPATU (Hibiscus rosa-sinensis L.) Murrukmihadi, Mimiek; Wahyuono, Subagus; Marchaban, Marchaban; Martono, Sudibyo
Majalah Obat Tradisional Vol 18, No 2 (2013)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (650.015 KB) | DOI: 10.14499/mot-TradMedJ18iss2pp%p

Abstract

Bunga kembang sepatu (Hibiscus rosa-sinensis L.) secara tradisional digunakan sebagai peluruh dahak. Berdasarkan atas Bioassay Guided Fractionation, fraksi aktif berhasil dipisahkan dan alkaloid merupakan kandungan utama fraksi. Oleh karena itu alkaloid digunakan sebagai senyawa penanda (marker) ekstrak etanol Hibiscus rosa-sinensis L. Nilai viskositas digunakan sebagai model untuk aktivitas peluruh dahak, dengan asetil sistein sebagai kontrol positif. Selanjutnya penetapan kadar alkaloid dalam ekstrak etanol dilakukan secara KLT-Densitometri (n=5), kadar alkaloid dibandingkan dengan kurva baku dari alkaloid (marker) hasil isolasi (Y=12,1360X+2901,4474). Kadar alkaloid dalam ekstrak etanol kembang sepatu (Hibiscus rosa-sinensis L.) sebagai 2,35 ± 0,67 %. 
Anti-Inflammatory Activity of Ethanol Extract of Cashew Stem Bark (Annacardium Occidentale L.) on Rat Paw Edema Induced by Carrageenan Harsini, Harsini; Sutardja, Iwa; Martono, Sudibyo; Sunarintyas, Siti; Sudarsono, Sudarsono
The Indonesian Journal of Dental Research Proceeding Book
Publisher : The Indonesian Journal of Dental Research

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Abstract

Introduction: Cashew stem bark (Anacardium occidentale L.) was traditionally used to cure inflammation in the oral cavity. Phenolic substances such as phenol and anacardic acid that have anti-inflammatory effect was found in cashew stem bark. The aim of this study was to investigate the anti-inflammatory activity of the ethanol extract of cashew stem bark and indometazine as Non-steroid anti-inflammatory drug. Materials and Methods: Cashew stem bark was collected from Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia. Extraction was done by maceration method using ethanol as solvent. Anti-inflammatory activity of 40 mg/kg bw, 80mg/kg bw, 160mg/kg bw dosage of cashew stem bark extract was monitored and indometazine 10 mg/kg bw was used as positive control. Edema volume determination on rat paw was counted as area under cure (AUC) value and anti-inflammatory percentage. Result: This study result showed that total phenolic content on cashew stem bark was 12.25 ± 0.26% w/w gallic acid equivalent (GAE). The anti-inflammatory activity of cashew stem bark extract in this study were 9.985±6.483% for 40mg/kg BW, 15.576±6.754% for 80mg/kg bw, 25.87±19.7% for 160mg/kg bw and 56.85 ±15.52% for Indometazine 10 mg/kg bw. Analysis of Variance (ANOVA) method was applied on the results and showed significant anti-inflammatory activity of ethanol extract on cashew stem bark (p<0.05). Conclusion: In conclusion, ethanol extract of cashew stem bark has anti-inflammatory activity. However, its’ activity is lower than indometazine.
Effect of aspirin on pi-class of rat kidney glutathione S-transferase activity Yuniarti, Nunung; Martono, Sudibyo; A.M., Supardjan
INDONESIAN JOURNAL OF PHARMACY Vol 16 No 2, 2005
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.919 KB) | DOI: 10.14499/indonesianjpharm0iss0pp87-93

Abstract

The nonsteroids antiinflammatory compounds like curcumin and its analogues, indomethacine, and sulfasalazin have been reported to have an inhibitory effect on GST activity on an in vitro study. The aim of this research is to find out the effect of aspirin on pi-class of rat kidney GST activity in vitro using ethacrynic acid (EA) as a specific substrate for its GST class.Glutathione activity can be measured by conjugating GSH and EA catalyzed with GST. The product can be measured spectrophotometrically to result in a rate (Δ absorption/min). With the same method, aspirin was added as an inhibitor. Decreasing conjugation product indicated that there was an inhibitory activity of aspirin.The aspirin inhibitory activity using EA and CDNB (1-chloro-2,4-dinitrobenzene) as substrates are 9,090% (Extrapolated IC50 6665,03 μM) and 14,087% (Extrapolated IC50 4102,0 μM), respectively. These results have shown that aspirin can not inhibit pi-class of rat kidney cytosolic GST activity using EA and CDNB (1-chloro-2,4-dinitrobenzene) as substrates.Key words: aspirin, GSTP, kidney.
PENGARUH 1,5-BIS(4’-HIDROKSI-3’-METOKSIFENIL)-1,4-PENTADIEN-3-ON DAN KURKUMIN PADA AKTIVITAS ENZIM GLUTATION S-TRANSFERASE PARU TIKUS Yuniarti, Nunung; Martono, Sudibyo
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 3, No 1 (2006)
Publisher : Indonesian Research Gateway

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Abstract

Curcumin was proved to have activity as GST inhibitor. 1,5-bis(4’-hydroxy-3’-methoxyphenyl)-1,4-pentadien-3-on which is curcumin analog, was predicted to have  same activity as curcumin. This experiment was conduct to verify that prediction, aimed to revealed the influence of 1,5-bis(4’-hydroxy-3’-methoxyphenyl)-1,4-pentadien-3-on as well as curcumin on rat lung GST activity  in vitro, using  1-chloro-2,4-dinitrobenzene (CDNB) as substrate. GST activity was determined using conjugation reaction of glutathione (GSH) with CDNB. GS-DNB conjugate formed was measured using spectrophotometry at l 345 nm between 0-3 minutes by simple kinetic program, result in certain rate (D absorption/minute). Using similar method, conjugation reaction was done, however with addition of 1,5-bis(4’-hydroxy-3’-methoxyphenyl)-1,4-pentadien-3-on and curcumin as inhibitor. Inhibition effect followed by the decreasing of reaction rate.  From the result it was concluded that  1,5-bis(4’-hydroxy-3’-methoxyphenyl)-1,4-pentadien-3-on and curcumin can inhibit mice lung GST activity with  IC50 value 9,13 mM and 13,32 mM. ABSTRAK Kurkumin dilaporkan memiliki aktivitas sebagai inhibitor GST. Senyawa 1,5-bis(4’-hidroksi-3’-metoksifenil)-1,4-pentadien-3-on yang merupakan senyawa analog kurkumin, diprediksikan memiliki aktivitas yang relatif sama dengan kurkumin. Oleh karena itu, dari hasil prediksi tersebut perlu dilakukan verifikasi dengan uji laboratoris. Penelitian ini bertujuan untuk mengetahui pengaruh senyawa 1,5-bis(4’-hidroksi-3’-metoksifenil)-1,4-pentadien-3-on dan kurkumin pada aktivitas GST paru tikus dengan substrat 1-kloro-2,4-dinitrobenzen (CDNB) secara in vitro. Aktivitas GST ditentukan menggunakan reaksi konjugasi glutation (GSH) dengan substrat CDNB. Konjugat GS-DNB yang terbentuk diukur secara spektrofotometri pada l 345 nm antara menit ke 0-3 menggunakan program simple kinetic, menghasilkan suatu rate (D serapan/menit). Dengan cara yang sama dilakukan reaksi konjugasi namun dengan penambahan senyawa 1,5-bis(4’-hidroksi-3’-metoksifenil)-1,4-pentadien-3-on dan kurkumin sebagai inhibitor. Adanya efek inhibisi diketahui dari penurunan rate. Dari hasil penelitian dapat disimpulkan bahwa senyawa 1,5-bis(4’-hidroksi-3’-metoksifenil)-1,4-pentadien-3-on dan kurkumin menghambat GST paru tikus dengan IC50 berturut-turut adalah 9,13 mM dan 13,32 mM.
Penetapan Kadar Alkaloid Ekstrak dari Etanolik Bunga Kembang Sepatu (Hibiscus rosa-sinensis L.) Murrukmihadi, Mimiek; Wahyuono, Subagus; Marchaban, .; Martono, Sudibyo
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 6, No 3 (2013)
Publisher : Indonesian Research Gateway

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Abstract

Kembang sepatu flower (Hibiscus rosa-sinensis L.) was fractionally used as expectorant. Based on Bioassay Guided fractionation, an active fraction was separated, and the fraction was identified is Alkaloid was the major compound based on TLC analysis. Viscosity value measured by viscometer was used as a Bioassay model of expectorant activity in vitro and acetyl cysteine was used as positive control. Alkaloid content determination of the ethanolic extract was measured by TLC-Densitometric compared with standard curve of isolated alkaloid as the selected marker (Y=12,1360X+2901,4474). The alkaloid content in the ethanolic extract was determined as 2.35±0,67%.Keywords : alkaloid, ethanolic extract, Hibiscus rosa-sinensis L.
Validasi Metode Penetapan Kadar Lisinopril dalam Spiked Plasma Secara Ultra Performance Liquid Chromatography Melalui Derivatisasi dengan 1-Fluoro 2,4 Dinitrobenzen Sumiyani, Ririn; Martono, Sudibyo; Sugiyanto, .
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 8, No 1 (2016)
Publisher : Indonesian Research Gateway

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Abstract

ABSTRACT: A rapid, accurate, and sensitive method for determining lisinopril in spiked plasma was developed by means using an Ultra Performance Liquid Chromatography (UPLC) with 1-fluoro 2,4 dinitrobenzen (FDNB) derivatization. Lisinopril was precolumn derivatized with FDNB at optimum condition, i.e. room temperature and borate buffer at pH 11, subsequently analyzed with UPLC. Isocratic condition of acetate buffer (0.01 M, pH 3.50) : acetonitrile : metanol = 70 : 10 : 20 (v/v/v) as mobile phase, 0.3 mL/min of flow rate at λ 296 nm were applied at Acquity BEH C18 column, resulting a linearity of lisinopril at range of concentration of 5,0-100 ng/mL (Y = 410,59x + 211,91, r = 0.93). The accuration of the established method was achieved by 88,59±6,01 to 101,70± 2,56% recovery, while the precision was shown with RSD value of 2,57- 8,16 %, limit of detection (LOD) instrument of 0,73 ng/mL and limit of quatification (LOQ) 2,44 ng/mL, dwith R2 = 0,9987 dan r = 0,9993. In addition, the resulted LOD and LOQ more or less similar with the published HPLC-MS-MS method (1.03-10.0 ng/mL). Hence, it could be concluded that the developed UPLC method can be used as an alternative method for determining lisinopril in plasma.Keywords: Lisinopril, FDNB, derivatization, UPLCABSTRAK: Penetapan kadar lisinopril dalam spiked plasma secara Ultra Performance Liquid Chromatography (UPLC) melalui derivatisasi dengan 1-fluoro 2,4 dinitrobenzen (FDNB) merupakan metode yang cepat, sensitif dan akurat. Derivatisasi precolumn lisinopril dan FDNB optimum pada suhu kamar, suasana dapar borat pH 11,0, dilanjutkan analisis secara UPLC isokratis menggunakan kolom Acquity BEH C dan fase gerak dapar asetat (0,01 M pH 3,50): asetonitril:metanol (70: 10: 20, v/v/v), laju alir 0,3 mL/menit pada λ 296 nm, menghasilkan linieritas kadar lisinopril dalam spiked plasma pada rentang 5,0 -100 ng/mL terhadap luas area lisinopril-DNB dengan persamaan Y = 410,59x + 211,91 dengan R2 = 0,9987 dan r = 0,9993 Akurasi metode ditunjukkan dengan nilai % rekoveri sebesar 88,59±6,01 smpai dengan 101,70± 2,56 %. Ketelitian ditunjukkan dengan nilai RSD 2,57- 8,16 %, sedangkan Batas Deteksi Instrumen = 0,73 ng/ mL dan Batas Kuantitasi = 2,44 ng/mL. Hasil Batas Deteksi penelitian ini relatif sama dengan Batas Deteksi penetapan kadar lisinopril secara HPLC-MS (1,03- 10,0 ng/mL). Dengan demikian dapat disimpulkan bahwa metode ini berpotensi dikembangkan sebagai metode alternatif pengganti HPLC-MS untuk penetapan lisinopril dalam plasma.Kata kunci: Lisinopril, FDNB, derivatisasi, UPLC
PENGARUH EKSTRAK KULIT BATANG JAMBU METE (Anacardium occidentale Linn.) SEBAGAI BAHAN ANTISEPTIK TERHADAP EKSPRESI VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) PADA LUKA BEKAS PENCABUTAN GIGI MARMUT: INFLUENCE OF CASHEW STEM BARK EXTRACT (Anacardium occidentale Linn.) AS AN ANTISEPTIC ON VEGF EXPRESSION AFTER TOOTH EXTRACTION OF THE GUINEA PIG Harsini; Iwa Sutardjo; Sudibyo Martono; Siti Sunarintyas; Sudarsono
Dentika: Dental Journal Vol. 18 No. 1 (2014): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.243 KB) | DOI: 10.32734/dentika.v18i1.1940

Abstract

Pencabutan gigi dapat berdampak pada penghambatan kontinuitas jaringan yang memerlukan waktu untukkembali sembuh. Vascular Endhotelial Growth Factor (VEGF) merupakan salah satu mediator pro inflamasiyang berhubungan dengan pembentukkan pembuluh darah baru. Kulit batang jambu mete (Anacardiumoccidentale Linn.) mempunyai kandungan fenolik yang dapat berperan pada pengaturan fungsi sel denganmenginduksi VEGF. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi ekstrak kulit batang jambumete sebagai bahan antiseptik terhadap ekspresi VEGF pada luka dampak pencabutan gigi hewan coba(marmut/Guinea Pig). Kulit batang jambu mete diambil dari Mojolegi, Karangtengah, Imogri, Bantul. Ekstraksidilakukan secara maserasi dengan pelarut etanol 70%. Bahan antiseptik dibuat dalam konsentrasi 3%, 5% dan 7%. Sebanyak 48 ekor marmut jantan, umur sekitar 2,5-3 bulan dengan bobot badan rata-rata 250-300 grdibagi menjadi 4 kelompok dan setiap kelompok terdiri atas 12 ekor. Seluruh marmut dilakukan pencabutangigi insisivus bawah kanan. Kelompok I adalah kelompok kontrol yang ditetesi air suling. Kelompok II ditetesiekstrak kulit batang jambu mete dengan konsentrasi 3%. Kelompok III ditetesi ekstrak kulit batang jambu mete5% dan kelompok IV ditetesi ekstrak kulit batang jambu mete 7%. Dekapitasi tiap kelompok sebanyak 4 ekordilakukan pada hari ke 3, 7dan 14. Setelah dekapitasi, soket bekas gigi diambil dan dibuat preparat histologisnyadengan pengecatan imunohistokimia. Penghitungan ekspresi VEGF dilakukan dibawah mikroskop denganperbesaran 40x. Data yang diperoleh dianalisis menggunakan Analisis Varians dua jalan dilanjutkan denganLSD0,05. Hasil penelitian menunjukkan antar konsentrasi, antar hari, dan interaksi antar konsentrasi dan hariberpengaruh signifikan terhadap ekspresi VEGF (p< 0,05). Pada uji LSD diketahui terdapat perbedaan ekspresiVEGF yang signifikan antar kelompok konsentrasi dan kelompok hari. Sebagai kesimpulan, ekstrak kulit batangjambu mete dalam bahan kumur berpengaruh terhadap ekspresi VEGF.
Validasi Metode Penetapan Kadar Lisinopril dalam Spiked Plasma Secara Ultra Performance Liquid Chromatography Melalui Derivatisasi dengan 1-Fluoro 2,4 Dinitrobenzen Sumiyani, Ririn; Martono, Sudibyo; Sugiyanto, .
Jurnal Farmasi Indonesia Vol 8, No 1 (2016)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (418.293 KB) | DOI: 10.35617/jfi.v8i1.417

Abstract

ABSTRACT: A rapid, accurate, and sensitive method for determining lisinopril in spiked plasma was developed by means using an Ultra Performance Liquid Chromatography (UPLC) with 1-fluoro 2,4 dinitrobenzen (FDNB) derivatization. Lisinopril was precolumn derivatized with FDNB at optimum condition, i.e. room temperature and borate buffer at pH 11, subsequently analyzed with UPLC. Isocratic condition of acetate buffer (0.01 M, pH 3.50) : acetonitrile : metanol = 70 : 10 : 20 (v/v/v) as mobile phase, 0.3 mL/min of flow rate at λ 296 nm were applied at Acquity BEH C18 column, resulting a linearity of lisinopril at range of concentration of 5,0-100 ng/mL (Y = 410,59x + 211,91, r = 0.93). The accuration of the established method was achieved by 88,59±6,01 to 101,70± 2,56% recovery, while the precision was shown with RSD value of 2,57- 8,16 %, limit of detection (LOD) instrument of 0,73 ng/mL and limit of quatification (LOQ) 2,44 ng/mL, dwith R2 = 0,9987 dan r = 0,9993. In addition, the resulted LOD and LOQ more or less similar with the published HPLC-MS-MS method (1.03-10.0 ng/mL). Hence, it could be concluded that the developed UPLC method can be used as an alternative method for determining lisinopril in plasma.Keywords: Lisinopril, FDNB, derivatization, UPLCABSTRAK: Penetapan kadar lisinopril dalam spiked plasma secara Ultra Performance Liquid Chromatography (UPLC) melalui derivatisasi dengan 1-fluoro 2,4 dinitrobenzen (FDNB) merupakan metode yang cepat, sensitif dan akurat. Derivatisasi precolumn lisinopril dan FDNB optimum pada suhu kamar, suasana dapar borat pH 11,0, dilanjutkan analisis secara UPLC isokratis menggunakan kolom Acquity BEH C dan fase gerak dapar asetat (0,01 M pH 3,50): asetonitril:metanol (70: 10: 20, v/v/v), laju alir 0,3 mL/menit pada λ 296 nm, menghasilkan linieritas kadar lisinopril dalam spiked plasma pada rentang 5,0 -100 ng/mL terhadap luas area lisinopril-DNB dengan persamaan Y = 410,59x + 211,91 dengan R2 = 0,9987 dan r = 0,9993 Akurasi metode ditunjukkan dengan nilai % rekoveri sebesar 88,59±6,01 smpai dengan 101,70± 2,56 %. Ketelitian ditunjukkan dengan nilai RSD 2,57- 8,16 %, sedangkan Batas Deteksi Instrumen = 0,73 ng/ mL dan Batas Kuantitasi = 2,44 ng/mL. Hasil Batas Deteksi penelitian ini relatif sama dengan Batas Deteksi penetapan kadar lisinopril secara HPLC-MS (1,03- 10,0 ng/mL). Dengan demikian dapat disimpulkan bahwa metode ini berpotensi dikembangkan sebagai metode alternatif pengganti HPLC-MS untuk penetapan lisinopril dalam plasma.Kata kunci: Lisinopril, FDNB, derivatisasi, UPLC
Co-Authors . Sugiyanto A. Margono, Supardjan Abdul Rohman Abdul Rohman Achmad Fudholi Achmad Fudholi Agung Endro Nugroho Agus Siswanto Agus Siswanto Agustinus Yuswanto, Agustinus Akhmad Kharis Nugroho Anak Agung Istri Sri Wiadnyani Any Guntarti Beti Pudyastuti Endang Lukitaningsih Etty Sulistyowati Harsini Harsini ,, Harsini I Dewa Gde Mayun Permana Iwa Sutardja, Iwa Iwa Sutardjo Iwa Sutardjo, Iwa Marchaban Marchaban, Marchaban Marchaban, . Marchaban, . Melania Perwitasari, Melania Mimiek Murrukmihadi Mochammad Imron Awalludin Nina Salamah Nunung Yuniarti Purwanto Purwanto Reyna Nuvitasari Ririn Sumiyani Riyanto, Sugeng Rohman, Abdul SATRIYAS ILYAS Siti Sunarintyas Siti Zahliyatul Munawiroh Subagus Wahyuono Sudarsono Sudarsono Sugeng Riyanto Supardjan A.M., Supardjan Supardjan Amin M Susilowati, Sri Sutji Wiranti Sri Rahayu Yuny Erwanto