BIOTRANSFORMATION OF CINCHONA ALKALOID COMPOUNDS BY MOLD Xylaria sp.TO CINCHONA N-OXIDE ALKALOIDS [Studies on Endophytic Microbes of Cinchona spp. plants (5)]

Partomuan Simanjuntak (Biotechnology Center Science Institute of Indonesia Jalan Raya Bogor Km 46 Cibinong 16911)
Bustanussalam . (Biotechnology Center Science Institute of Indonesia Jalan Raya Bogor Km 46 Cibinong 16911)
Titik K. Prana (Biotechnology Center Science Institute of Indonesia Jalan Raya Bogor Km 46 Cibinong 16911)
K. Ohashi (Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Japan)
Hirotaka Shibuya (Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Japan)

INDONESIAN JOURNAL OF PHARMACY
Vol 13 No 2, 2002

A biotransformation study on alkaloid chinchona compounds (quinine, quinidine, cinchonidine, cinhonine) by endophytic microbe from Cinchona succirubra have been done. The biotransformation for 2 days after preculture yielded cinchona N-oxide compounds (quinine N-oxide, quinidine N-oxide, cinchonidine N-oxide, cinchonine N-oxide) alkaloids and the bioassay of the compounds also showed effectiveness as antimalaria. Purification the biotransfomed compound was conducted by a high performance liquid chromatograph (HPLC) and determination of chemical structure was done based on carbon nuclear magnetic resonance (NMR).Key words : Biotransformation, endophyte microbe, Xylaria sp., Cinchona succirubra Pav., cinchona alkaloid, quinine, quinine N-oxide