Tracheospasmolytic activity of vitetrifolin-E isolated from the leaves of Vitex trifolia L.

Alam, G. (Department of Pharmacy, Hasanuddin University, Makassar, Indonesia)
Gandjar, I.G. (Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, Indonesia)
Hakim, Lukman (Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, Indonesia)
Timmerman, H. (Division of Pharmacochemistry, Leiden/Amsterdam Center for Drug Research, Vrije Universiteit, Amsterdam, the Netherlands)
Veerporte, R. (Division of Pharmacognosy, Leiden/Amsterdam Center for Drug Research, Leiden University, the Netherlands)
Wahyuono, S. (Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, Indonesia)

INDONESIAN JOURNAL OF PHARMACY
Vol 14 No 4, 2003

In searching of tracheospasmolytic active compounds from Vitex trifolia, we are now reporting isolation and identification of vitetrifolin-E from active fraction obtained from n-hexane extract of V. trifolia. Vitetrifolin-E blocked spontaneous contraction of male guinea pig trachea induced by histamine and was active in a model using sensitized guinea pig trachea stimulated by ovalbumin. Vitetrifolin-E inhibited tracheal contraction by 22.6% at dose of 1.3 x 10-4 M and by 86.1% at dose of 4.10-4 M. In a model using sensitized male guinea pig trachea, vitetrifolin-E also inhibited the contraction 83.5% (1.3 x 10-5 M).Keywords: vitetrifolin-E, Vitex trifolia, tracheospasmolytic