International Journal of Health Science and Technology
Vol. 6 No. 1 (2024): July

Inhibition of Gliocladium sp against plant pathogenic fungus and their exoenzyme activity

Fardhani, Dinar Mindrati (Unknown)
Fuadiyah, Dzakiya Alda (Unknown)
Susila, Wisnu Adhi (Unknown)
Nugraheni, Ika Afifah (Unknown)



Article Info

Publish Date
22 Jul 2024

Abstract

Gliocladium sp. It is known to have an antagonistic mechanism against other organisms by means of hyper parasitism, antibiosis and lysis, as well as competition. This study aims to determine the effectiveness of Gliocladium sp. in inhibiting several types of plant pathogenic fungi and their exoenzyme activity. Inhibition testing was carried out by the Dual Culture Assay method on several plant pathogenic fungi including Colletotrichum gloeosporioides, Rhizoctonia solani, Fusarium sp., and Phytophthora sp. The exoenzyme activity tested includes cellulolytic tests, amylolytic tests, and chitinolytic tests. Antagonist testing showed that Gliocladium sp. able to inhibit the growth of all pathogenic fungi tested with varying percentage of inhibition. High percentage of inhibition was shown in the pathogens Colletotrichum gloeosporioides and Rhizoctonia solani with percentages of 78.75% and 75%, respectively. Then, the antagonist activity against Fusarium sp. has a moderate resistance percentage of 56%. Meanwhile, a low percentage of inhibition is shown in Gliocladium sp. against Phytophthora sp. with a figure of 25%. From the characterization of exoenzyme activity in Gliocladium sp., negative results were obtained in liquid and solid Carboxymethyl Cellulose, amylum, and chitin media. Meanwhile, in the amylolytic test, positive growth was marked by the appearance of mycelia on the surface of the amylum liquid medium, but in the solid media negative results were obtained against the amylase enzyme. Gliocladium sp fungus has the potential to be a biocontrol agent against various plant pathogenic fungi

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Journal Info

Abbrev

ijhst

Publisher

Subject

Humanities Engineering Health Professions Nursing Public Health

Description

International Journal of Health Science and Technology published since July 2019 with ISSN Online: 2685-8673 by LPPM Universitas Aisyiyah Yogyakarta. IJHST Published third a year in January, July and November. This journal contains research papers as results of research which have already been ...