Production, Characterization, and Toxicity Test of L-asparaginase from Vibrio alginolyticus Bacterial Symbiont of Green Algae Caulerpa lentillifera

Kasturiasih, Ni Putu
Ahmad, Ahyar
Arfah, Rugaiyah A.
Khairunnur, Siti
Soekamto, Nunuk Hariani
Taba, Paulina
Hala, Yusafir
Karim, Harningsih

Molekul: Jurnal Ilmiah Kimia
Vol 18 No 3 (2023)

L-asparaginase is an enzyme that can be used as an anticancer therapeutic agent. This study aimed to optimize fermentation time for production, optimization, and to test the toxicity of L-asparaginase from Vibrio alginolyticus (V. alginolyticus) bacterial symbiont of green algae Caulerpa lentillifera (C. lentillifera). The activity of L-asparaginase enzyme assay and the toxicity test were done by using the Nessler method and the Brine Shrimp Lethality Test (BSLT) method respectively. The results show L-asparaginase with 66 hours of fermentation time indicates the highest enzyme activity. L-asparaginase mentioned has an optimum enzyme activity of 17.99 U/mL for 30 minutes of incubation time at 37 °C, and pH 7.5. The BSLT results show LC50 value of 17.83 μg/mL indicating the enzyme is bio-toxic with high-level toxicity, which can continue for cytotoxicity tests on cancer cells.