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All Journal Indonesia Chimica Acta Molekul: Jurnal Ilmiah Kimia
Khairunnur, Siti
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology

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Production, Characterization, and Toxicity Test of L-asparaginase from Vibrio alginolyticus Bacterial Symbiont of Green Algae Caulerpa lentillifera Kasturiasih, Ni Putu; Ahmad, Ahyar; Arfah, Rugaiyah A.; Khairunnur, Siti; Soekamto, Nunuk Hariani; Taba, Paulina; Hala, Yusafir; Karim, Harningsih
Molekul Vol 18 No 3 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.3.8153

Abstract

L-asparaginase is an enzyme that can be used as an anticancer therapeutic agent. This study aimed to optimize fermentation time for production, optimization, and to test the toxicity of L-asparaginase from Vibrio alginolyticus (V. alginolyticus) bacterial symbiont of green algae Caulerpa lentillifera (C. lentillifera). The activity of L-asparaginase enzyme assay and the toxicity test were done by using the Nessler method and the Brine Shrimp Lethality Test (BSLT) method respectively. The results show L-asparaginase with 66 hours of fermentation time indicates the highest enzyme activity. L-asparaginase mentioned has an optimum enzyme activity of 17.99 U/mL for 30 minutes of incubation time at 37 °C, and pH 7.5. The BSLT results show LC50 value of 17.83 μg/mL indicating the enzyme is bio-toxic with high-level toxicity, which can continue for cytotoxicity tests on cancer cells.
The Toxicity and Cytotoxicity Test of Anticancer Peptides Isolated from Symbiotic Bacteria Asmi, Nur; Ahmad, Ahyar; Natsir, Hasnah; Karim, Harningsih; Muhakim, Ali; Khairunnur, Siti
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Vol. 18 No. 1 (2025): Volume 18, No 1: June 2025
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70561/ica.v18i1.43925

Abstract

The purpose of this research was to evaluate the toxicity and cytotoxicity of the fragment peptide from symbiotic bacteria in the lung cancer LK-2 cell line, compared normal M5S cell line. Protein hydrolyzates were separated using Molecular Weight Cut Off (MWCO) to isolate peptide fragments. Fragments were categorized as ≤ 5 kDa, > 5-10 kDa, and > 10 kDa to evaluate their toxicity through the Brine Shrimp Lethality Test (BSLT) method and to study cytotoxicity effects and proliferation in LK-2 lung cancer cells against normal M5S cells. MTT assay was used to analyze the percentage of living cells and cell growth. The Selectivity Index (SI) equation was applied to determine selectivity. Findings indicated that the peptide fragment ≤ 5 kDa had the highest toxicity, with an LC50 of 8.15 µg/ml. The number of LK-2 cells that died increased when exposed to peptide fragments of sizes ≤ 5 kDa, > 5-10 kDa, and > 10 kDa over 16 hours. Moreover, cell growth in LK-2 cells decreased after 16 hours, while there was no significant decline (P < 0.05) in M5S cells compared to the controls. The peptide fragments with molecular weights ≤ 5 kDa and > 5-10 kDa demonstrated the highest selectivity, resulting in an SI value of 3. These results suggest that the selected peptide fragments may serve effectively as anticancer agents. This study highlights the potential of peptides from symbiotic bacteria as possible treatments for cancer, particularly lung cancer.
Co-Authors Ahyar Ahmad Ali Muhakim Arfah, Rugaiyah A. Harningsih Karim Hasnah Natsir Kasturiasih, Ni Putu Nunuk Hariani Soekamto Nur Asmi Paulina Taba Yusafir Hala