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All Journal Jurnal Ilmu Lingkungan Journal of Tropical Biodiversity and Biotechnology Indonesia Chimica Acta Emerging Science Journal Indonesian Journal of Chemistry Indobiosains JURNAL PENGABDIAN MASYARAKAT YAMASI Molekul: Jurnal Ilmiah Kimia
Harningsih Karim
Department of Pharmacy, School of Pharmacy Yamasi Makassar, 90222, South Sulawesi
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Production, Characterization, and Toxicity Test of L-asparaginase from Vibrio alginolyticus Bacterial Symbiont of Green Algae Caulerpa lentillifera Kasturiasih, Ni Putu; Ahmad, Ahyar; Arfah, Rugaiyah A.; Khairunnur, Siti; Soekamto, Nunuk Hariani; Taba, Paulina; Hala, Yusafir; Karim, Harningsih
Molekul Vol 18 No 3 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.3.8153

Abstract

L-asparaginase is an enzyme that can be used as an anticancer therapeutic agent. This study aimed to optimize fermentation time for production, optimization, and to test the toxicity of L-asparaginase from Vibrio alginolyticus (V. alginolyticus) bacterial symbiont of green algae Caulerpa lentillifera (C. lentillifera). The activity of L-asparaginase enzyme assay and the toxicity test were done by using the Nessler method and the Brine Shrimp Lethality Test (BSLT) method respectively. The results show L-asparaginase with 66 hours of fermentation time indicates the highest enzyme activity. L-asparaginase mentioned has an optimum enzyme activity of 17.99 U/mL for 30 minutes of incubation time at 37 °C, and pH 7.5. The BSLT results show LC50 value of 17.83 μg/mL indicating the enzyme is bio-toxic with high-level toxicity, which can continue for cytotoxicity tests on cancer cells.
Bioactivity and Metabolites Compounds of Medicinal Plants Endophytic Fungi in Indonesia Sukmawaty, Eka; Karim, Abdul; dwyana, Zarsawaty; Natsir, Hasnah; Karim, Harningsih; Ahmad, Ahyar
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.79070

Abstract

Indonesia is rich in diversity of medicinal plants, vital in traditional medicine and the pharmaceutical industry. However, overharvesting, along with population growth, land use changes, deforestation, and climate change, endanger these plants. This review investigates the potential of endophytic endosymbiont as an alternative. These endosymbionts can synthesise bioactive compounds similar to those found in medicinal plants.  This study compiled data from various sources on endophytic fungi and their bioactivity. The review aims to categorise Indonesian medicinal plants, to identify their associated endophytic fungi from different plant parts, and to assess their bioactivity. The results revealed numerous medicinal plant families and a variety of endophytic fungi isolated from fruits, leaves, twigs, bark, roots, and rhizomes. These fungi exhibited bioactivities, including antioxidant, anticancer, antidiabetic, and antimicrobial effects, with metabolites such as alkaloids, flavonoids, peptides, phenols, polyketides, quinones, steroids, and terpenoids. Fusarium and Colletotrichum were the most common endophytic fungi found. Notably, the biological activity was consistent among endophytic fungi from various host organs, but variations were observed according to the host's geographical origin. This suggests that Indonesia's diverse geography influences metabolite production and activity. However, the same host plant may harbour different species in distinct organs. These findings indicate that endophytic fungi within medicinal plants represent a promising source of bioactive compounds for future Indonesian medicine production. Future research should explore metabolite compounds and bioactivity across different geographical regions. 
Synthesis and Characterization of Nickel Nanoparticles: Biological and Photocatalytic Properties Fadliah, Fadliah; Raya, Indah; Ahmad, Ahyar; Taba, Paulina; Burhanuddinnur, Muhammad; Gaffar, Maming; Putri, Tri Widayati; Nur, Arfiani; Abubakar, Andi Nur Fitriani; Munadi, Rachmin; Majid, Ahmad Fudhail; Pratama, Irham; Yasser, M.; Jarre, Sulistiani; Karim, Harningsih
Indonesian Journal of Chemistry Vol 25, No 1 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.90034

Abstract

The potential uses of ecologically benign nickel nanoparticle manufacturing in various sectors, such as biomedicine, energy storage, and catalysis, have garnered much interest. This paper covers green approaches to nickel nanoparticle manufacturing, which integrate natural substances as stabilizing and reducing agents with eco-friendly processes. Phytochemicals derived from bacteria, microorganisms, and plant leaf extracts can convert nickel ions (Ni2+) into nickel nanoparticles. Room temperature is used for the synthesis procedure, and neither dangerous compounds nor unusual reaction conditions are used. Using a variety of analytical methods, the resultant nickel nanoparticles were characterized. We also investigate the possibility of using the generated nickel nanoparticles as a cytotoxic, photocatalytic, antioxidant, and antibacterial agents. The antimicrobial activity of nickel nanoparticles demonstrates their potent antibacterial properties, while their antioxidant activity demonstrates their capacity to combat free radicals effectively. Furthermore, nickel nanoparticles' cytotoxic activity demonstrates their capacity to kill cancer cells, and their photocatalytic activity demonstrates their efficiency in breaking down organic contaminants. This review highlights the value of ecologically benign synthetic methods and creates new avenues for developing nickel nanoparticle applications in health and the environment.
A Review of Marine Bacterial Intracellular and Extracellular Bioactive Compounds as Novel Antibacterial and Anti-Inflammation Agents Karim, Harningsih; Azis, Arief; Ramadani, Ananda; Anita, Anita; Ahmad, Ahyar; Natsir, Hasnah; Taba, Paulina; Putri, Suriati Eka; Sarlan, Sarlan; Larekeng, Siti Halimah; Irfandi, Rizal
Indonesian Journal of Chemistry Vol 25, No 1 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.90039

Abstract

Unique and varied bioactive compounds produced by the ocean have drawn attention and served as a focus for creating antibacterial and anti-inflammatory agents. As part of the approach for locating these research sources, databases such as PubMed, Science Direct, MDPI, Google Scholar, Springer Link, Web of Science, Scopus, and Wiley Online Library were used to identify completed studies. Numerous intriguing bioactive compounds have so far been isolated from marine bacteria. A crucial resource in the ongoing search for novel peptides, proteins, lipids, nucleosides, enzymes, alkaloids, polyketides, and terpenoids is the diversity of marine bacterium strains. This review summarizes several bacterial intracellular and extracellular bioactive compounds that have been applied as antibacterial and anti-inflammatory agents in 2016–2024, which we present in the form of structures, species sources, and evaluations of these compounds' antibacterial and anti-inflammatory activities. Therefore, this review article can be used as a recommendation for utilizing biomaterials from marine bacteria that are promising in the future for industrial-scale production of antibacterial and anti-inflammatory agents.
The Utilization of Gracilaria sp. as Raw Material for Cellulose in Cellulose Acetate-Nickel Oxide (CA-NiO) as Electrode for Energy Storage Technology Karim, Abdul; Ahmad, Ahyar; Arfah, Rugaiyah Andi; Ermawar, Riksfardini Annisa; Karim, Harningsih; Putra, Andi Erwin Eka; Putri, Suriati Eka; Negara, Satria Putra Jaya; Larekeng, Siti Halimah
Indonesian Journal of Chemistry Vol 25, No 1 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.96533

Abstract

In the modern era, electrical energy has become a significant need that drives the large consumption of fossil fuels and their environmental impacts. Supercapacitors can reduce this large consumption of natural polymers such as cellulose acetate (CA), which can be synthesized from Gracilaria sp. Composites with CA can be an environmentally friendly alternative electrode with low toxicity. The results show that the cellulose has been successfully synthesized from the algae Gracilaria sp., which was proven by FTIR spectra analysis. The results also show that supercapacitor electrodes have been successfully made where the manufactured electrodes form a composite between CA and nickel oxide (NiO), with the highest specific capacitance and specific energy values of 15.5 × 102 F/g and 13.3 × 102 Wh/kg, respectively, on the CA-NiO2 electrode, but when the NiO concentration is higher than the CA concentration the specific capacitance and specific energy decrease as shown on the CA-NiO electrode with NiO mass of 0.6 g. Thus, the materials results of this study can be applied to electric vehicles and technology that requires electricity storage in the future.
Optimasi Pertumbuhan Bakteri Laut Penghasil Protease Menggunakan Variasi Konsentrasi Media Skim Milk Modifikasi Anita, Anita; Natsir, Hasnah; Ahmad, Ahyar; Taba, Paulina; Karim, Harningsih
Indobiosains 2025: Volume 7 Nomor 1 Februari 2025
Publisher : Universitas PGRI Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31851/indobiosains.v7i1.17822

Abstract

This study aims to analyze the growth rate of marine bacteria producing protease using variations in the concentration of skim milk media and to observe the relationship between cell growth and enzyme activity. The study was conducted using three concentrations of skim milk media (1%, 2%, and 3%) and observation of growth and enzyme activity for 108 hours. The results showed that 1% skim milk concentration produced the largest proteolytic zone diameter (13.8 mm) with the fastest growth (1 day). Observations of cell growth patterns and enzyme activity showed that the highest enzyme activity (0.5933 U/mL) occurred in the early growth phase and decreased in subsequent phases even though cell growth increased. This indicates that there is no linear relationship between cell growth and enzyme activity in marine bacteria-producing protease, where enzyme production and activity are influenced by various complex factors such as physiological conditions of the cells and nutrient availability.
The Toxicity and Cytotoxicity Test of Anticancer Peptides Isolated from Symbiotic Bacteria Asmi, Nur; Ahmad, Ahyar; Natsir, Hasnah; Karim, Harningsih; Muhakim, Ali; Khairunnur, Siti
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Vol. 18 No. 1 (2025): Volume 18, No 1: June 2025
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70561/ica.v18i1.43925

Abstract

The purpose of this research was to evaluate the toxicity and cytotoxicity of the fragment peptide from symbiotic bacteria in the lung cancer LK-2 cell line, compared normal M5S cell line. Protein hydrolyzates were separated using Molecular Weight Cut Off (MWCO) to isolate peptide fragments. Fragments were categorized as ≤ 5 kDa, > 5-10 kDa, and > 10 kDa to evaluate their toxicity through the Brine Shrimp Lethality Test (BSLT) method and to study cytotoxicity effects and proliferation in LK-2 lung cancer cells against normal M5S cells. MTT assay was used to analyze the percentage of living cells and cell growth. The Selectivity Index (SI) equation was applied to determine selectivity. Findings indicated that the peptide fragment ≤ 5 kDa had the highest toxicity, with an LC50 of 8.15 µg/ml. The number of LK-2 cells that died increased when exposed to peptide fragments of sizes ≤ 5 kDa, > 5-10 kDa, and > 10 kDa over 16 hours. Moreover, cell growth in LK-2 cells decreased after 16 hours, while there was no significant decline (P < 0.05) in M5S cells compared to the controls. The peptide fragments with molecular weights ≤ 5 kDa and > 5-10 kDa demonstrated the highest selectivity, resulting in an SI value of 3. These results suggest that the selected peptide fragments may serve effectively as anticancer agents. This study highlights the potential of peptides from symbiotic bacteria as possible treatments for cancer, particularly lung cancer.
PELATIHAN PEMBUATAN SABUN CUCI PIRING DAN SABUN CUCI PAKAIAN DI DAERAH BONTONOMPO KABUPATEN GOWA Yusriyani; Thahir, Zakiah; A. Tenriugi Daeng Pine; Karim, Harningsih; Agust Dwi Djajanti; Base, Nurul Hidayah; Basir, Hernawati; Sukirawati; Noena, Raymond Arief N; Maulana, Maulana Zulkarnain Imansyah
JURNAL PENGABDIAN MASYARAKAT YAMASI Vol. 2 No. 2 (2023): Jurnal Pengabdian Masyarakat Yamasi
Publisher : Akademi Farmasi Yamasi Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59060/jpmy.v2i2.308

Abstract

Kegiatan pembuatan sabun cair cuci piring dan sabun cuci pakaian merupakan hal yang menarik bagi masyarakat karena dapat menambah pengetahuan mengenai cara pembuatan sabun. Sabun cuci piring merupakan bahan yang telah menjadi kebutuhan sehari-hari terutama bagi ibu-ibu rumah tangga. Mahalnya produk sabun cuci piring di pasaran dapat diatasi dengan pembuatan sabun cuci piring sendiri dengan kualitas yang cukup baik dan lebih ekonomis yang dapat digunakan untuk kebutuhan sehari-hari maupun untuk dijual. Dengan adanya sabun pembersih yang dibuat sendiri. Hal ini dapat mengurangi pengeluaran rumah tangga untuk membeli sabun pembersih pasaran karena sabun yang dibuat lebih murah dan produk sabun yang dihasilkan lebih banyak. Tujuan kegiatan ini adalah memberikan edukasi dan pelatihan pembuatan sabun cuci piring dan sabun cuci pakaian yang aman digunakan kepada masyarakat daerah Katangka Kabupaten Gowa. Metode yang dilakukan pada kegiatan ini berupa penyuluhan dan pemberian tutorial pembuatan sabun cuci piring yang disimak langsung oleh peserta yang hadir. Jumlah peserta yang hadir dalam kegiatan pengabdian ini sebanyak 39 orang yang keseluruhannya adalah warga Desa Katangka Kecamatan Bontonompo Kabupaten Gowa. Kegiatan ini memberikan hasil berupa peningkatan pengetahuan dan keterampilan kepada masyarakat mengenai pembuatan produk sabun cuci piring dan sabun cuci pakaian yang aman digunakan. Kegiatan ini berjalan dengan lancar sesuai dengan yang direncanakan. Peserta sangat antusias mengikuti pelatihan yang dapat menambah keterampilan dan sekaligus menciptakan wirausaha baru dan mandiri dan menjadikanya sebagai peluang bisnis. Berdasarkan kegiatan yang telah dilakukan, dapat disimpulkan bahwa pelatihan pembuatan sabun cuci piring dan sabun cuci pakaian dapat menambah pengetahuan dan edukasi masyarakat Desa Katangka Kecamatan Bontonompo Kabupaten Gowa dalam pembuatan produk sabun cuci yang aman.
LITERASI BERBAGAI OBAT HERBAL DAN CARA TEPAT PENGGUNAANNYA UNTUK KESEHATAN A Tenriugi Daeng Pine; Tahirah; Yasnidar; Sitti Fauziah Noer; Mora; Karim, Harningsih; Idrus, Irman; Dwi Sasongko Mardiono; Junaidin
JURNAL PENGABDIAN MASYARAKAT YAMASI Vol. 2 No. 2 (2023): Jurnal Pengabdian Masyarakat Yamasi
Publisher : Akademi Farmasi Yamasi Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59060/jpmy.v2i2.310

Abstract

Sistem pelayanan kesehatan tradisional merupakan bagian dari sistem kesehatan nasional. Saat ini kesehatan tradisional diatur dalam Peraturan Pemerintah (PP) Nomor 103 tahun 2014 Tentang Pelayanan Kesehatan Tradisional. Penggunaan tanaman sebagai obat telah dikenal sejak lama oleh masyarakat Indonesia. Hal ini menjadi warisan turun-temurun yang masih dapat ditemui dan masih dilestarikan di wilayah Indonesia. Salah satu wilayah Indonesia yang memiliki keragaman hayati adalah Pangkep (Pangkajene Kepulauan). Kekayaan alam ini dapat diinformasikan dan dikembangkan untuk meningkatkan kesejahteraan masyarakat daerah Pangkep dan sekitarnya salah satunya sebagai bahan pengobatan. Adanya penggunaan tanaman obat (herbal) yang masih kurang tepat dapat memberikan efek samping terhadap kesehatan di masa depan. Oleh karena itu, kegiatan ini bertujuan untuk memperkenalkan beberapa tanaman yang dapat digunakan sebagai bahan obat-obatan tradisional dan penggunaannya yang aman dan tepat. Metode yang digunakan dalam kegiatan PKM ini adalah dalam bentuk penyuluhan yang disertai dengan diskusi. Kegiatan ini memberikan dampak positif yakni adanya peningkatan pengetahuan masyarakat Kelurahan Bonto Perak Kecamatan Pangkajene Kabupaten Pangkep terhadap berbagai herbal dan penggunaannya yang aman dan tepat.
A Review: Application of Bioremediation and Phytoremediation Techniques for Heavy Metal Contamination in Mining Areas of Sulawesi, Indonesia Azis, Fitria; Nurfadini, Nurfadini; Ahmad, Ahyar; Febryanti, Amalyah; Sappewali, Sappewali; Ardita R, Dian Fitrah; Asmi, Nur; Karim, Harningsih
Jurnal Ilmu Lingkungan Vol 23, No 5 (2025): September 2025
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jil.23.5.1423-1440

Abstract

Mining has been a practice spanning millennia, leaving behind a legacy of mine waste containing hazardous substances, including various metals in nearly every country. Indonesia is one of the most nickel-rich regions in the world. Some of these resources are located in the Sulawesi and North Maluku regions. Sulawesi includes Central Sulawesi, South Sulawesi and Southeast Sulawesi. Pollutants in the form of mercury metal (Hg), lead metal (Pb), cadmium metal (Cd), and chromium metal (Cr) are considered toxic and difficult to remove due to their stable nature. Effective remediation methods are imperative in response to this issue, among which bioremediation and phytoremediation stand out. Bioremediation employs microorganisms to detoxify the environment of heavy metals, while phytoremediation utilizes plants for the same purpose. Both methods involve intricate interactions among plants, microbes, and their substrates to purify the environment and facilitate further filtration. Bioremediation encompasses two primary approaches: the in-situ method and the ex-situ method. In situ methods include bioventing, biosparging, bioaugmentation, and biostimulation. Ex-situ methods, contaminated soil or water is retrieved from its origin and purified elsewhere by living organisms; this process utilizes bioreactors, aeration, steam regulation, and additional nutrients to increase the rate of pollutant decomposition. The bioremediation mechanism involving microorganisms employs biosorption, bioaccumulation, biotransformation, and bioleaching. Phytoremediation employs several methods, including phytoextraction, phytofiltration, phytovolatilization, phytodegradation, and phytodesalination. Identifying efficient hyperaccumulators is the most direct approach for successful heavy metal phytoremediation. Bioremediation and phytoremediation techniques are economical and suitable solutions to address heavy metal pollution in polluted environments. With a deeper understanding of these techniques, it is hoped that we can more effectively address these complex environmental challenges, particularly in mining areas in Sulawesi, Indonesia.
Co-Authors A. Tenriugi Daeng Pine A. Tenriugi Daeng Pine Abdul Karim Abubakar, Andi Nur Fitriani Agust Dwi Djajanti Ahmad Fudhail  Majid Ahyar Ahmad Ali Muhakim Andi Erwin Eka Putra Anita Anita Ardita R, Dian Fitrah Arfah, Rugaiyah Arfah, Rugaiyah A. Arfah, Rugaiyah Andi Arfiani Nur Azis, Fitria Base, Nurul Hidayah Basir, Hernawati BUDI SAKSONO Burhanuddinnur, Muhammad Deni Zulfiana Dwi Sasongko Mardiono dwyana, Zarsawaty Ermawar, Riksfardini A. Fadliah, Fadliah Febryanti, Amalyah Hasnah Natsir Indah Raya Irfandi, Rizal Irman Idrus Irmawati Junaidin, Junaidin Kasturiasih, Ni Putu Khairunnur, Siti Maming Gaffar, Maming Maulana, Maulana Zulkarnain Imansyah Mora Munadi, Rachmin Negara, Satria Putra Jaya Noena, Raymond Arief N Nunuk Hariani Soekamto Nur Asmi Nurfadini, Nurfadini Paulina Taba Putri, Tri Widayati Ramadani, Ananda Ramli, Siti R. Ridwan, Irham Pratama Riksfardini Annisa ERMAWAR Sappewali, Sappewali Sarlan, Sarlan Sita Heris Anita SITI HALIMAH LAREKENG, SITI HALIMAH Sitti Fauziah Noer Sukirawati Sukmawaty, Eka Sulistiani Jarre Suriati Eka Putri Tahirah Thahir, Zakiah yasnidar Yasser, M Yusafir Hala Yusriyani Zainuddin, Rahmaniah