<![CDATA[The research aimed to develop an appropriate cognitive assessment for scientific literacy to measure science education students' abilities to identify learning strengths and weaknesses. This research used the R & D method. The normality test and a one-sample t-test was conducted. The specific topic covered in the research is fermented material. The study participants are science education students at a public university in Surabaya, Indonesia. The results indicated that the percentage of question difficulty levels, with 11.11% categorized as difficult and 44.44% categorized as easy. The analysis of test item discrimination reveals that 66.67% fall into the very good category, 11.11% into the good category, and 22.22% into the bad category. The one-sample t-test is conducted, yielding a significance value of 0.0314. Based on the obtained data, with 0.314 > 0.05, the null hypothesis (Ho) is accepted. This implies that the TOSLS instrument influences assessing students' level of scientific literacy ability. The findings of this study serve as a reference and best practice for tertiary institutions seeking to evaluate students' scientific literacy abilities.]]>
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