Innovations in the production of soap products continue to be developed, such as paper soap with the addition of nanochitosan, which has antibacterial benefits. This study aimed to obtain nanosized chitosan and determine the best formula for antibacterial paper soap with the addition of mangrove crab shell nanochitosan (Scylla sp.) through physicochemical and microbiological characterization. The manufacture of nanochitosan was using ionic gelation. The formulation of antibacterial paper soap with the addition of nanochitosan (0, 0.25, 0.5, 0.75, and 1%). The results showed that Scylla sp. shell nanochitosan had a size of 67.07 nm. Paper soap with the addition of Scylla sp. nanochitosan (0, 0.25, 0.5, 0.75, and 1%) produced pH value of 9.18-9.27, water content 18.25-32.00% and free alkali 18.25-32.00%. All paper soap concentrations had a fine texture, jasmine aroma, and beige color. The gram-positive bacteria Staphylococcus aureus in paper soap produces a clear zone (3.5-14 mm and gram-negative bacteria are able to inhibit Echerichia coli 4.50-11.50 mm. Antibacterial paper soap with a nanochitosan concentration of 0.5% was the best formulation for inhibiting the growth of gram-positive bacteria (S. aureus) and a concentration of 0.75% for gram-negative bacteria (E. coli). The antibacterial paper soap with the addition of shell nanochitosan (Scylla sp.) in this study met the soap criteria according to the SNI standards.
                        
                        
                        
                        
                            
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