<![CDATA[Liquid waste from oil industry activities has potential cause environmental pollution.These liquid wastes, containing hydrocarbon and heavy metals, are mostly toxic. There-fore, biotechnology by means of biological treatment can be applied for decomposing thetoxic liquid wastes. The biosurfactant production frorn some microorganisms can supporthydrocarbon degradation. The objective of this study is to examine the crude biosurfactantthat is extracted frorn the selected Bacillus which was precipitated by using methanol onacid moiety. The crude biosurfactant extract were tested to support hydrocarbon degrada-tion. Three species of Bacillus used in this experiment were compared based on their re-spective biosurfactant production. The results showed that the percentage of hydrocarbondegradation on liquid waste frorn refinery by the three Bacillus species were 90.23% (Ba-cillus subtilis), 88.72% (Bacillus licheniformis), and 73.43%> (Bacillus laterosporus). Theconcentration of remaining oil after 28days was 20.44 mg/L, 23.38 mg/L, and 54.87 mg/L,respectively. The decrease of COD were 84.90%, 84.04%, and 80.68%, respectively, andthe COD value after 28days treatment were 165 mg/L, 174 mg/L, and 211 mg/L.]]>
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