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All Journal Microbiology Indonesia Arkus Scientific Contribution Oil and Gas LEMBARAN PUBLIKASI MINYAK DAN GAS BUMI
LENI HERLINA
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Religion Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Energy Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Social Sciences

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Construction and Expression of Single Recombinant Peptide Surfactant for EOR Application CUT NANDA SARI; USMAN USMAN; RIESA KW ROHMAT; LENI HERLINA; KEN SAWITRI SULIANDRI; ONIE KRISTIAWAN; DWIYANTARI DWIYANTARI; TATI KRISTIANTI; SONY SUHANDONO
Microbiology Indonesia Vol. 11 No. 1 (2017): March 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1420.163 KB) | DOI: 10.5454/mi.11.1.5

Abstract

Surfactant is generally synthetic chemical, which is effective and reliable. However, the chemicals usually did not degraded easily in the environment and could cause damage to the environment. The other possible alternative to produce surfactant is using genetic engineering in order to produce peptide based surfactant. In this research, peptide surfactant was produced using a gene construct which was created using overlapped polymerase chain reaction method (OE-PCR). PAGE analysis shows that single surfactant peptide construction can be expressed by induction of IPTG 1 mM and after at least twice sonication. This research proves that both two constructions have been successfully expressed by producing peptide in expected size (approximately 15 kDa).
IN SILICO POTENTIAL ANALYSIS OF X6D MODEL OF PEPTIDE SURFACTANT FOR ENHANCED OIL RECOVERY Cut Nanda Sari; Usman; Rukman Hertadi; Tegar Nurwahyu Wijaya; Leni Herlina; Ken Sawitri Suliandari; Syafrizal; Onie Kristiawan
Scientific Contributions Oil and Gas Vol. 39 No. 2 (2016): SCOG
Publisher : Testing Center for Oil and Gas LEMIGAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29017/scog.39.2.267

Abstract

Peptides and their derivatives can be applied in enhanced oil recovery (EOR) due to their ability to form an emulsion with hydrophobic molecules. However, peptide research for EOR application, either theoretical or computational studies, is still limited. The purpose of this research is to analyse the potency of the X6D model of surfactant peptide for EOR by molecular dynamics simulations in oil-water interface. Molecular dynamics simulation using GROMACS Software with Martini force field can assess a peptides ability for self-assembly and emulsification on a microscopic scale. Molecular dynamics simulations combined with coarse grained models will give information about the dynamics of peptide molecules in oil-water interface and the calculation of interfacial tension value. Four designs of X6D model: F6D, L6D, V6D, and I6D are simulated on the oil-water interface. The value of interfacial tension from simulation show the trend of F6D L6D > I6D > V6D. The results indicate that V6D has the greatest reduction in interfacial tension and has the stability until 90C with the salinity of at least 1M NaCl.
CONSTRUCTION AND EXPRESSION?OF QUARTET RECOMBINANT PEPTIDE SURFACTANT FOR EOR APPLICATION Cut Nanda Sari; Usman; Refiana Lestary; Riesa Khairunnisa W.R.; Leni Herlina; Syafrizal; Tati Kristianti; Sony Suhandono
Scientific Contributions Oil and Gas Vol. 39 No. 3 (2016): SCOG
Publisher : Testing Center for Oil and Gas LEMIGAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29017/scog.39.3.271

Abstract

The main drawback of the SUPEL peptide surfactant product which has been developed for EOR application is it isunstable at a high temperature. This research is aimed at generating the prototype of peptide surfactant construction in recombinant by stringing up 4 SUPEL linier sequences. Quartet recombinant technology can produce the peptide surfactant characterized as reversible biosurfactant, which is active at high temperature but inactive at low temperature. Multiple SUPEL Construction (MSC) that was developed in this research is using synthetic DNA and producing SUPEL in 4 sequences that can flip at normal temperature and can open when heated. SDS PAGE analysis results show that MSC construction can be expressed by inducting IPTG and cell harvested at 90C. This research proves that construction and expression of the SUPEL quartet has been achieved by producing the peptide at an ideal size.
Effect Of Biosurfactant Produced B Y Bacillus In Oily Wastewater Degradation Sri Kadarwati; Leni Herlina
Scientific Contributions Oil and Gas Vol. 31 No. 3 (2008): SCOG
Publisher : Testing Center for Oil and Gas LEMIGAS

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Abstract

Liquid waste from oil industry activities has potential cause environmental pollution.These liquid wastes, containing hydrocarbon and heavy metals, are mostly toxic. There-fore, biotechnology by means of biological treatment can be applied for decomposing thetoxic liquid wastes. The biosurfactant production frorn some microorganisms can supporthydrocarbon degradation. The objective of this study is to examine the crude biosurfactantthat is extracted frorn the selected Bacillus which was precipitated by using methanol onacid moiety. The crude biosurfactant extract were tested to support hydrocarbon degrada-tion. Three species of Bacillus used in this experiment were compared based on their re-spective biosurfactant production. The results showed that the percentage of hydrocarbondegradation on liquid waste frorn refinery by the three Bacillus species were 90.23% (Ba-cillus subtilis), 88.72% (Bacillus licheniformis), and 73.43%> (Bacillus laterosporus). Theconcentration of remaining oil after 28days was 20.44 mg/L, 23.38 mg/L, and 54.87 mg/L,respectively. The decrease of COD were 84.90%, 84.04%, and 80.68%, respectively, andthe COD value after 28days treatment were 165 mg/L, 174 mg/L, and 211 mg/L.
PENGEMBANGAN METODE IDENTIFIKASI KARAKTERISTIK MINYAK BERAT HASIL EKSTRAKSI OIL SAND ILIRAN HIGH DENGAN FORMULA PERHITUNGAN BERDASARKAN KOMPOSISI ELEMENTER Muhammad Fuad; Dwi Endah Rachmawati; Leni Herlina; Daliya Indra Setiawan; Rezky Iriansyah Anugrah
Lembaran Publikasi Minyak dan Gas Bumi Vol. 56 No. 2 (2022): LPMGB
Publisher : BBPMGB LEMIGAS

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Abstract

Sampai saat ini, minyak bumi masih memiliki peran strategis sebagai sumber energi bagi aktivitas kehidupan manusia. Akan tetapi, kebutuhan minyak bumi tidak diimbangi dengan cadangan yang ada. Oleh karena itu, minyak non-konvensional seperti minyak berat Iliran High perlu dikembangkan lebih jauh sebagai salah satu sumber cadangan minyak masa depan. Identifikasi sifat karakteristik minyak ini sangat penting karena menjadi tolok ukur mutu dan potensi umpan kilang minyak bumi. Namun identifikasi minyak hasil ekstraksi oil sand tidak mudah, karena sifatnya yang berat, sulit mengalir serta hasil (yield) yang sedikit. Minyak Iliran High diperoleh dengan mengekstrak oil sand menggunakan berbagai pelarut organik. Larutan hasil ekstraksi lalu dipisahkan dari padatan dengan proses filtrasi (penyaringan). Untuk merekoveri minyak dari pelarut dilakukan proses evaporasi sehingga diperoleh ekstrak berupa minyak berat dan rafinat (pelarut yang sudah tidak mengandung minyak) yang dapat digunakan kembali (diregenerasi) untuk proses ekstraksi selanjutnya. Kemudian dilakukan pengujian laboratorium untuk mengetahui specific gravity, gross heat value dan komposisi elementer. Berdasarkan data tersebut, dapat dilakukan identifikasi minyak berat Iliran High denfgan pendekatan rumus perhitungan untuk menentukan sifat karakteristik minyak berat seperti: rasio H/C, nilai kalor, bobot molekul, titik didih rerata, nilai faktor KUOP, titik anilin, titik tuang, viskositas, residu karbon, kandungan aspalten dan kadar aromatik. Karakterisasi minyak Iliran High menunjukkan komposisi elementer C, H, O, N dan S masing-masing sebesar 85,39 – 88,12%wt, 11,34 – 12,26%wt, 0,10 – 0,12%wt, 0 – 2,55%wt, dan >0,01%wt. Minyak Iliran High merupakan minyak berat, sweet crude dan dikategorikan sebagai minyak naftenik, dengan kadar aromatik ~40%.
Sintesis dan Karakterisasi Kopolimer Lateks Karet Alam dengan Stirena dan Anhidrida Maleat sebagai Penurun Titik Tuang Minyak Mentah Roza Adriany; Dwi Supriningsih; Devitra Saka Rani; Abdul Haris; Efa Yenti; Leni Herlina; Rudi Suhartono; Herizal; Gitta Prawidia
Lembaran Publikasi Minyak dan Gas Bumi Vol. 56 No. 3 (2022): LPMGB
Publisher : BBPMGB LEMIGAS

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Abstract

Aditif penurun titik tuang minyak mentah umumnya dibuat dari bahan petrokimia yang merupakan sumber tidak terbarukan. Oleh sebab itu, ketersediaan aditif dari sumber terbarukan sangat diperlukan untuk mengurangi ketergantungan terhadap bahan petrokimia. Penelitian ini bertujuan untuk mendapatkan aditif penurun titik tuang minyak mentah berbahan dasar Lateks Karet Alam. Pembuatan aditif ini menggunakan reaksi kopolimerisasi isoprena yang terkandung di dalam Lateks Karet Alam dengan monomer anhidrida maleat dan monomer stirena dengan rasio 70:20:10 (b/b), 70:15:15 (b/b), dan 70:10:20 (b/b) hingga terbentuk kopolimer. Kopolimer tersebut kemudian ditambahkan ke dalam dua jenis minyak mentah pada variasi konsentrasi 1000 ppm, 5000 ppm dan 10.000 ppm. Analisis yang dilakukan meliputi uji karakteristik lateks karet alam, uji fisika dan kimia minyak mentah, serta uji titik tuang minyak mentah sebelum dan sesudah penambahan kopolimer. Hasil penelitian menunjukkan bahwa penurunan pour point maksimum terjadi pada penambahan kopolimer 10.000 ppm dengan rasio Lateks Karet Alam, anhidrida maleat, dan stirena adalah 70:20:10. Pada rasio dan konsentrasi ini, kopolimer mampu menurunkan titik tuang minyak mentah dari 45°C menjadi 33°C
Pengaruh Perubahan beberapa Parameter terhadap Pemisahan Komponen memakai Kromatografi - Gas Soetandar Soekapradja; Leni Herlina
Lembaran Publikasi Minyak dan Gas Bumi Vol. 24 No. 2 (1990): LPMGB
Publisher : BBPMGB LEMIGAS

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Abstract

Ada berbagai macam cara untuk memisahkan komponen-komponen suatu contoh, dan teknik kromotografi adalah salah satu di antaranya. Keberhasilan pemisahan dengan teknik ini sangat tergantung kepada parameter-parameter yang dipergunakan dalam operasi itu.
The Effect of Effleurage Massage Technique on Reducing the Scale of Uterine Involution Pain in First Day of Postpartum Mothers in the Working Area of Cikadu Health Center, Cianjur Regency, Indonesia Leni Herlina; Handayani, Teti Yuli
Arkus Vol. 9 No. 2 (2023): Arkus
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/arkus.v9i2.388

Abstract

The effleurage technique can increase blood circulation to the area being massaged. Increasing blood flow to the uterus and lower abdominal area can help in postpartum recovery and reduce the pain of uterine involution. This study aimed to determine the effect of effleurage massage techniques on reducing the scale of uterine involution pain in postpartum mothers on day 1 in the Cikadu Health Center Working Area, Cianjur Regency, Indonesia. This research is an experimental study with a one-group pretest-posttest design approach. It uses primary data obtained using an assessment instrument, the numeric rating scale (NRS), to determine the level of back pain in pregnant women. A total of 30 research subjects participated in this study. This study shows that effleurage massage intervention effectively reduces the intensity of uterine involution pain in postpartum mothers. There was a decrease in pain intensity after massage effleurage, and it was stated to be statistically different, p<0.05. In conclusion, there is an effect of the effleurage massage technique on reducing the scale of uterine involution pain in postpartum mothers on day 1 in the Cikadu Health Center Working Area, Cianjur Regency, Indonesia
Co-Authors Abdul Haris CUT NANDA SARI Daliya Indra Setiawan Devitra Saka Rani Dwi Endah Rachmawati Dwi Supriningsih DWIYANTARI DWIYANTARI Efa Yenti Gitta Prawidia Handayani, Teti Yuli Ida Ayu Putu Sri Widnyani Ken Sawitri Suliandari KEN SAWITRI SULIANDRI MUHAMMAD FUAD ONIE KRISTIAWAN Refiana Lestary Rezky Iriansyah Anugrah Riesa Khairunnisa W.R. RIESA KW ROHMAT Roza Adriany Rudi Suhartono RUKMAN HERTADI Soetandar Soekapradja SONY SUHANDONO Sri Kadarwati syafrizal TATI KRISTIANTI Tegar Nurwahyu Wijaya Usman Usman Usman