Jurnal Biologi Tropis
Vol. 24 No. 1 (2024): Januari - Maret

Screening of Antimicrobial Producing Bacteria from Berawe Beach Sand on Kampai Pangkalan Susu Island against Pathogenic Bacteria

Annisa, Rafika (Unknown)
Manalu, Kartika (Unknown)
Nasution, Rizki Amelia (Unknown)



Article Info

Publish Date
11 Jan 2024

Abstract

Sand is naturally a place to live, grow and develop some marine animals and microorganisms, usually bacteria originating from the sea area that have a large number of bioactive compounds that can produce various kinds of secondary metabolites for further production of antimicrobials. The purpose of this study was to obtain bacteria that have the potential to produce antimicrobials and to characterize bacterial isolates on the sand of Berawe Beach, Kampai Island, Pangkalan Susu. In this study several stages were carried out, namely sampling, isolation, purification, morphological characterization, antimicrobial activity test, gram staining, biochemical test and determining the bacterial genus. The results of this study obtained 9 isolates (SP14A. SP15A, SP16A, SP24A, SP24B, SP35A, SP44A, SP44B and SP45A) which have the potential as antimicrobial producers. Characterization of antimicrobial bacterial isolates from the sand of Berawe Beach, Kampai Island, Pangkalan Susu, namely isolate SP14A, which is a gram- positive bacterium in the form of streptobacilli. SP15A, SP24B and SP44B isolates were coccus-shaped gram-positive bacteria. SP16A, SP35A, SP44A and SP45A isolates were gram-positive bacteria in the form of bacilli. And isolate SP24A is a gram-negative bacterium in the form of streptobacilli. All isolates produce catalase enzymes but do not use carbon and energy. Isolates that are motile (SP14A, SP15A, SP16A, SP24A, SP35A, SP44A and SP45A) can ferment glucose while non-motile (SP24B and SP44B) cannot ferment glucose. These bacteria come from the genera Bacillus, Micrococcus, LactoBacillus and Alcaligenes. Species of Bacillus pumilus, Bacillus subtilis, Bacillus firmus, LactoBacillus bulgaricus, Micrococcus luteus and Alcaligenes eutrophus.

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Journal Info

Abbrev

JBT

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Description

Jurnal Biologi Tropis (ISSN Cetak 1411-9587 dan ISSN Online 2549-7863) diterbitkan mulai tahun 2000 dengan frekuensi 2 kali setahun oleh Program Studi Pendidikan Biologi PMIPA FKIP Universitas Mataram, berisi hasil penelitian dan ulasan Ilmiah dalam bidang Biologi ...