This study seeks to evaluate the validity and reliability of the outcomes derived from the adoption of the Representational Systems and Chemical Reactions Diagnostic Instrument (RSCRDI). This research employs a quantitative, survey-based approach, involving 69 prospective chemistry teachers at a university in Indonesia. Prior to implementation, the instrument was translated from English to Indonesian using the back-translation method. The collected data were subsequently analyzed using Winstep, yielding strong validity and reliability results. The instrument exhibited a Cronbach's alpha of 0.65, a person reliability of 0.60, and an item reliability of 0.76. Furthermore, the construct validation results were favorable, as evidenced by mean score (MNSQ), Z-standard (ZSTD), and point measure correlation (Pt Mea Corr) indices. These findings indicate that the RSCRDI is a valid and reliable instrument for assessing conceptual understanding among prospective chemistry teachers in Indonesia.
                        
                        
                        
                        
                            
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