This study aims to evaluate the effect of olive oil supplementation in commercial feed on the growth and survival rate of milkfish (Chanos chanos) fry. The research used a Completely Randomized Design with five treatments and three replications: control without oil addition (A), fish oil 1 ml/30 g feed (B), olive oil 0.5 ml/30 g feed (C), olive oil 1 ml/30 g feed (D), and olive oil 1.5 ml/30 g feed (E). The results showed that olive oil supplementation significantly affected growth and survival (p<0.05). Treatment D (olive oil 1 ml/30 g feed) demonstrated the best performance with absolute weight growth of 0.40 g, relative weight growth of 366.7%, specific daily weight growth rate of 3.48%/day, and relative length growth percentage of 172.2%. This treatment resulted in the highest survival rate of 86.7%. These values were significantly higher compared to other treatments, including fish oil supplementation. These findings indicate that olive oil supplementation at a dose of 1 ml/30 g feed can effectively optimize the growth and survival of milkfish fry, and has the potential to become a sustainable alternative to fish oil in aquaculture feed formulations.
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