Prosiding Seminar Kesehatan Masyarakat
Vol 2 No October (2024): Proceeding of The 1st International Seminar on Public Health and Sports (IS

Detection of Pig DNA Contamination in Facial Creams Containing Collagen Using Conventional PCR

Fitria, Meutia Srikandi (Unknown)
Dewi, Juliana Permata (Unknown)
Mukaromah, Ana Hidayati (Unknown)



Article Info

Publish Date
30 Oct 2024

Abstract

Background: Cosmetics are pharmaceutical preparations intended to support appearance or care for the body. Both local and imported facial whitening products are mostly marketed at varying prices, from cheap to expensive, making more people buy them. The supply of collagen extracted from mammals has recently been limited and is mostly obtained from the tendons and skin of cattle and pigs. The collagen content not listed on cosmetic packaging makes people worried about pork collagen being contaminated, therefore detection is needed to find out. This research aimed to detect whether there was pork contamination in facial cream products containing collagen with Cytochrome b (Cyt-b) primers using conventional PCR. Method: The research design is exploratory. The samples used were 5 facial cream samples and one positive control. Samples were subjected to DNA extraction, DNA concentration quantification, PCR, and DNA electrophoresis. Result: In the positive control, a DNA band of ~149 bp specifically targeting Cytochrome b was formed, while the facial cream sample did not form a DNA band. Conclusion: The five facial creams used as samples were not contaminated with pork contamination.

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Journal Info

Abbrev

prosidingfkm

Publisher

Subject

Dentistry Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

Description

Prosiding Seminar Kesehatan Masyarakat (PRIMA KESMAS) merupakan media publikasi naskah peserta kegiatan Seminar Hasil Penelitian dan Pengabdian Masyarakat Bidang Ilmu Kesehatan dan Olahraga yang diselenggarakan setiap bulan Agustus oleh Fakultas Kesehatan Masyarakat Universitas Muhammadiyah ...