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All Journal PROSIDING SEMINAR NASIONAL JURNAL KESEHATAN El-Hayah : Jurnal Biologi Biosaintifika: Journal of Biology & Biology Education Bali Medika Jurnal JURNAL INOVASI DAN PENGABDIAN MASYARAKAT INDONESIA Prosiding Seminar Kesehatan Masyarakat Prosiding Seminar Nasional Unimus
Ana Hidayati Mukaromah
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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PHOTODEGRADATION OF Cr (VI) IN VARIOUS CONCENTRATION OF ZSM-5 IMPREGNATED TIO2 Alfiani, Yulia; Mukaromah, Ana Hidayati; Sulistyaningtyas, Ayu Rahmawati
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Nowadays,water could be contaminated by heavy metals such as chromium (Cr). Chromium toxicity depends on its state of valence. Cr (VI) as highly mobile is toxic, while Cr (III) as a less mobile is less toxic. Potentially, Cr (VI) content   can be reduced by ZSM-5 impregnated TiO2. TiO2  as a catalyst can activated and modified zeolite with active metal materials. The impregnation between ZSM-5 and TiO2 should be reduce Cr (VI) content in water. The objective of this study was to assess concentration of ZSM-5 impregnated TiO2 which  most  effectively  for  decreasing  Cr  (VI)  content.  Decreasing  Cr  (VI) content in various concentration of ZSM-5/TiO2  was measured using UV-Vis spectrophotometer. The results showed that variation of zeolite concentration had an effect on decreasing Cr (VI) content in water. Photodegradation of Cr (VI) in various concentrations (0.25%;0.50%; 0.75%; 1.00%; 1.25% w/v) respectively are 16.99%; 18.84%; 24.83%; 30.82%; 36.14%. Concentration 1.25% w/v of ZSM-5 impregnated TiO2   as the highest to decrease Cr (IV) content. On the other hand, Using TiO2  or ZSM-5 only was showing lower percentage of Cr (VI) reduction which compared ZSM-5/TiO2. In conclusion, More higher concentration ZSM-5/TiO2  caused   faster photodegradation and higher percentage decrease of Cr (VI) content in water. Keywords: Concentration, Cr (VI),  Photodegradation, TiO2, ZSM-5
Adsorption of Carbon Monoxide (CO) on ZSM-5 Membrane in Smoking Area Azizah, Inas Hasna; Mindhumalid, Tatut; Mukaromah, Ana Hidayati
El-Hayah : Jurnal Biologi Vol 6, No 2 (2017): EL-HAYAH (VOL 6, NO 2,March 2017)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v6i2.4533

Abstract

This research aimed to investigate the ability of ZSM-5 in absorbing cigarette smoke to reduce CO level in smoking area. The research method are according to Gao, 2011 and Mukaromah 2014. The results were characterized using x-ray diffraction (XRD), FTR and SEM. The absorbance test was conducted by placing the gauze absorbed into the enclosed space with volume 18.000 cm3containing CO from cigarette smoke. The research was reached the stage of inorganic and organic substances leaching and oxidized in electrochemistry in H2SO4 to 20%. Zeolite zsm-5 membrane has the ability to absorb CO with the decline in CO on the highest levels the surface area of 25 cm2 to the time contact 30 minutes is 0.50 %.
SINTESIS MEMBRAN ZEOLIT ZSM-5 SECARA COATING PADA SUHU 90 ° C BERDASARKAN VARIASI PERLAKUAN KASA STAINLESS STEEL AISI 316 180 MESH DALAM MENURUNKAN KADAR GAS CO Maulida Julia Saputri; Ana Hidayati Mukaromah; Yusrin Yusrin
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian dan Pengabdian Masyarakat
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Telah dilakukan sintesis membran zeolit ZSM-5 secara coating pada suhu 90o C berdasarkan variasi perlakuan kasa stainless-steel AISI 316 180 mesh terhadap penurunan kadar gas CO. Tujuan penelitian ini untuk mengetahui pengaruh membran zeolit ZSM-5 yang disintesis secara coating pada suhu 90 C berdasarkan variasi perlakuan kasa stainless-steel AISI 316 180 mesh terhadap penurunan kadar gas CO.Jenis penelitian ini merupakan eksperimen yang didukung studi pustaka. Sebelumnya dilakukan perlakuan agar membran zeolit pada saat coating dapat  tumbuh dengan baik. Kasa stainless steel AISI 316 ukuran 180 mesh diberikan perlakuan I dengan HNO 10% dan aceton, perlakuan II dengan Toluene 95% dan HCl 15%,perlakuan III dengan NaOH 15%;HCl 15% dan H32SO 20%, serta perlakuan IV dengan toluene 95%; HCl 5% dan TPABr 0,1 M.  Hasil penelitian menunjukkan persentase penurunan kadar gas CO berdasarkan variasi perlakuan I, II, III dan IV diperoleh rata-rata persentase penurunan kadar gas CO sebesar12,45±1,76%; 9,38±1,41 %; 15,07±1,05% dan 11,74±1,12 %. Kapasitas  adsorpsi membran  zeolit ZSM-5 kasa AISI 316 180 mesh terhadap gas CO dengan perlakuan I, II, III dan IV diperoleh rata-rata yaitu 34781,93±1230,6 mg/g; 17252,03±1295,17 mg/g; 81047,46±3809,98 mg/g dan 30681,42 ±1589,472mg/g.Persentase penurunan kadar gas CO serta adsorpsi membran zeolit terbaik pada perlakuan III. Hasil dari uji statistik One Way Anova didapat nilai signifikansi 0,004 < 0,05 , disimpulkan bahwa Terdapat pengaruh variasi perlakuan stainless steel dengan sintesis membran zeolit ZSM-5 secara coating pada suhu 90°C terhadap penurunan kadar gas CO.Kata Kunci : Zeolit ZSM-5, Perlakuan terhadap kasa, Coating, Kadar gas CO
DEGRADASI ZAT WARNA RHODAMIN B SECARA Advanced Oxidation Processes METODE FENTON BERDASARKAN VARIASI KONSENTRASI H2O2 Ana Hidayati Mukaromah; - Yusrin; Endah Mubiarti
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2012: SEMINAR NASIONAL HASIL PENELITIAN 2012
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Industri percetakan sebagai salah satu bidang industry dan sebagian besar industri percetakan skalakecil-menengah ini belum melakukan pengelolaan yang baik terhadap limbah cairnya. Hal iniberpotensi menimbulkan pencemaran dan kerusakan lingkungan terutama perairan. Teknologi AOPsmerupakan salah satu teknologi yang banyak dikembangkan dan selama ini telah berhasilmendegradasi/menyisihkan warna dari suatu limbah cair. Tujuan penelitian ini adalah adalahmendegradasi zat warna secara artificial yaitu zat warna Rhodamin B dalam kondisi optimum (waktupengolahan, dosis H2O2 dan Fe2+ , dan pH 3) menghasilkan efisiensi yang paling baik (maksimal)dalam skala laboratorium dan menghitung prosentase penurunan konsentrasi warna Rhodamin Bsetelah diolah menggunakan proses Fenton. Hasil penelitian adalah panjang gelombang optimumadalah 550 nm dan waktu reaksi optimum 15 menit.Kondisi optimum dalam mendegradasi zat warnaRhodamin B 100 ppm tercapai pada [Fe2+] 100 ppm dengan penambahan volume 10 mL yaitusebesar 23.54 % dengan pertimbangan lebih ekonomis. Pemakaian konsentrasi H2O2 dalam jumlahbesar akan meningkatkan tingkat degradasi zat warna Rhodamin B, akan tetapi pada konsentrasitertentu hal ini tidak berlaku karena terjadinya fenomena OH• Scavenger. Zat warna Rhodamin B 100ppm dengan 10 mL [Fe2+] 100 ppm dan konsentrasi H2O2 yang paling besar adalah 450 ppm selama120 menit terdegradasi 85,47 %.
PROFIL PROTEIN ULAT SAGU (Rhynchophorus ferrugenesis) YANG DIGORENG DAN DIPANGGANG MENGGUNAKAN METODE SDS-PAGE Noverson Lidaya; Stalis Norma Ethica; Ana Hidayati Mukaromah
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Sago larvae is one of typical foods in papua, which rich of proteins containing various  types  of  essential  amino  acids  and  having  economic  value.  Papuan people use sago larvae as a source of income and for consumption. The heat processing on the worm could lead to protein denaturation. The objective of this research was to investigate the characteristic change of protein band pattern on sago larvae sample by SDS-PAGE method. Results of the study showed that the process of frying with time variations of 2, 4 and 6 minutes was proved to cause denaturation of sago larvae protein indicated by the missing of protein bands observation on electrophoregram. The highest level of sago larvae protein denaturation occured on sample fried for 6 min. There were only 4 minor protein bands with molecular weight of 60 kDa, 42 kDa, 40 kDa, and 31 kDa. Observed on the roasting process, there was no significant change in protein profile of the sample roasted for 2 to 6 min, but sample roasted for 4-min appeared to begin losing protein band on gel. As conclusion based on the experiment performed, the best time for frying and roasting of sago larvae is 2 min. Keywords: Sago larvae, Frying, Roasting, Protein profile, SDS-PAGE.
A Novel Method for the Growth of ZSM-5 Membranes on Stainless steel Supports by coating on Reactor Vessel with Surface-to-volume Ratio of Synthesis Governing Low Temperature Crystallization of ZSM-5 Ana Hidayati Mukaromah; Tulus Ariyadi
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Proceeding 3rd ISET 2017 | International Seminar on Educational Technology 3rd 2017
Publisher : Universitas Muhammadiyah Semarang

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Abstract

ZSM-5 membranes have been prepared using a novel seeding method. By sonicating ZSM-5 seed crystals in the presence of a stainless steel mesh of size followed by a controlled low temperature synthesis step it has been shown by XRD, FTIR, and SEM-EDX. The growth of ZSM-5 zeolite at stainless steel can be used to produce ZSM-5 zeolite membranes on reactor vessel with surface-to-volume ratio was the highest of the reactor vessel. Surface-to-volume ratio influences the heat-transfer during the synthesis, which further governs the crystallization of ZSM-5. It was found that the higher the surface-to-volume of the reactor, the more crystalline the resulting product. The result for the theoretical review paper were necessary to research the synthesis of ZSM-5 membrane based on the variation of type and size stainless steel and pretreatment of thestainless steel to use as a membrane suppport. The ratio of surface area to volume of the first reactor is the highest 1.44 influence more effective heat transfer process and the crystallinity of the zeolite ZSM-5 in the synthesis of ZSM-5 zeolite.Keywords: ZSM-5 membrane, type and size of the stainless steel, pretreatment, vessel reactor.
ISOLASI BAKTERI PENGHASIL ENZIM PROTEASE STAPHYLOCOCCUS HOMINIS PADA ONCOM MERAH PASCA FERMENTASI 120 JAM Aulia Harun; Sakti Imam Muchlissin; Ana Hidayati Mukaromah; Sri Darmawati; Stalis Norma Ethica
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Enzymes are complex protein moleculer produced by living cells playing role as catalysts in various chemical processes in the body. Among enzymes playing an important role in human life is protease. The purpose of this study was to determine the presence of protease – producing bacteria found on 120-h post - fermented oncom and to identify the bacteria based on its 16S   rRNA gene analysis. Bacterial isolation and purification was carried out using Nutrient Agar media with spread technique. Of the six bacterial isolates isolated from the oncom sample after 120 hours of fermentation, there was one isolate that had protease activity, namely IROD 5. The protease enzyme income test was carried out using Skim Milk Agar media. Molecular identification process was carried out through sequential analysis of 16S rRNA using PCR method using primers forward F: 5'-AGAGTTGATCCTGGCTCAG-3 'and reverse R: 5'- GGTTACCTTGTTAC. GACTT-3 primers' followed by sequencing process. The protease enzyme production test to bacterial isolate was conducted using Skim Milk Agar. Molecular identification was performed through analysis of 16S rRNA gene sequence using PCR method followed by sequencing process. A single bacterial isolate having proteolytic activity was obtained based on observation of the clear zone of protease surrounding the bacterial colony with a diameter of 72 mm. The 16S rRNA gene sequence of the obtained proteolytic bacterial strain IROD5 has been obtained and analysis on the gene sequence resulted 99% similarity levels with sequence of similar gene s of Staphylococcus hominis. As conclusion, the obtained bacterial isolate in this studyis apotential protease  enzyme  producer  and  molecularly  identified  as  Staphylococcus hominis strains IROD5. Keyword : Protease Enzyme, Gen 16S rRNA, Red Oncom
ANALISIS KALIUM DAN PROSENTASE DAYA LARUT CALSIUM OKSALAT OLEH KALIUM DALAM AIR TEH DAUN SUKUN (Artocarpus altilis) Endang Triwahyuni Maharani; Ana Hidayati Mukaromah; Jatmiko Susilo
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2012: SEMINAR NASIONAL HASIL PENELITIAN 2012
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Sukun termasuk dalam genus Artocarpus (famili Moraceae) yang terdiri atas 50 spesies tanamanberkayu. Daun tanaman sukun mengandung beberapa zat berkhasiat seperti asam hidrosianat,asetilcolin, kalium, tanin, riboflavin, dan sebagainya. Zat-zat tersebut mampu mengatasi peradangan,menurunkan kolesterol, mengobati penyakit hati, inflamasi, jantung, ginjal dan pembuluh darah.Tujuan penelitian ini adalah menganalisis kadar kalium pada larutan daun sukun segar, kering, dandaun sukun yang diabukan, melakukan optimasi konsentrasi daun sukun kering dan berat Ca Oksalat(CaC2O4), serta menentukan daya larut Ca Oksalat (CaC2O4) oleh kalium dalam air rebusan teh daunsukun (Artocarpus altilis). Hasil penelitian adalah kadar kalium pada larutan daun sukun segar 560,22mg/kg, pada larutan daun sukun kering 573,68 mg/kg, dan pada daun sukun yang diabukan adalah872,68 mg/kg. Rata – rata daya larut Ca Oksalat dalam air rebusan teh daun sukun dengan frekuensisatu kali sehari selama tujuh hari adalah 81,73%. Ada pengaruh penggunaan air teh daun sukun satukali sehari selama tujuh hari berturut – turut terhadap prosentase daya larut kalsium oksalat.
PENURUNAN KONSENTRASI GAS KARBON MONOKSIDA DENGAN MEMBRAN ZEOLIT ZSM-5 SECARA COATING MENGGUNAKAN KASA AISI 316-180 MESH DAN 304-400 MESH DENGAN PERLAKUAN I DAN II Ana Hidayati Mukaromah; Tulus Ariyadi; Maulida Julia Saputri; Rinda Aulia Utami; Yusrin -
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian dan Pengabdian Masyarakat
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Tujuan penelitian ini adalah mensintesis membran zeolit ZSM-5 secara coating pada suhu 90 °C pada 2 jenis kasa yaitu kasa AISI 316-180 mesh dan kasa 304-400 mesh dengan 2 perlakuan yaitu perlakuan I dengan merendam kasa dalam HNO 31% kemudian direndam dalam aceton dan perlakuan II (merendam kasa dalam toluen 95% selama 12 jam kemudian dalam HCl 15%, dan  TPABr 0,1M dan mengaplikasikan membran zeolit ZSM-5 untuk menurunkan konsentrasi gas karbon monoksida di dalam ruangan. Metode sintesis membran ZSM-5 secara coating. Hasil penelitian tentang persentase penurunan kadar gas karbonmonoksida pada membran dengan kasa AISI 316-180 mesh dan 304-400 mesh perlakuan I (direndam dalam HNO31% kemudian dalam aceton) berturut-turut adalah 13,37±1,13 % dan 14,04±0,44 %, sedangkan dengan perlakuan 2 (direndam dengan Toluen 12 jam, dan HCl 5%,TPABr 0,1M)) berturut-turut adalah 11,23±0,64 % dan 12,81±0,58 %. Dengan demikian persentase penurunan kadar gas karbon monoksida tertinggi pada membran dengan kasa 304400 perlakuan I (direndam dalam HNO 31% kemudian dalam aceton).Kata kunci: Karbon monoksida, membran zeolit ZSM-5, coating, Tipe Kasa, Perlakuan
ISOLASI BAKTERI PENGHASIL ENZIM PROTEASE BACILLUS THURINGIENSIS IRODI PADA ONCOM MERAH PASCA FERMENTASI 24 JAM Radna Safitri; Sakti Imam Muchlissin; Ana Hidayati Mukaromah; Sri Darmawati; Stalis Norma Ethica
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Abstract

The need for protease enzymes in Indonesia and the world continues to increase, requiring  new  protease  sources.  Bacteria  are  beneficial  sources  of  protease because they are easy to obtain and rapidly multiply. Bacterial identification could be done molecularly through analysis of the 16S rRNA gene. This study aimed to obtain an isolate of protease-producing bacterium from 24-h post-fermented red oncom and to identify the obtained bacterial strain molecularly by 16S rRNA gene sequence. The protease production test on bacteria found in red oncom sample was done  using  a  selective  medium,  Skim  Milk  Agar  (SMA).  DNA  genomes  of proteolytic bacterial cells were extracted by Promega KIT. The amplifying process of 16S rRNA gene using the Polymerase Chain Reaction (PCR) method. The amplified  DNA  were  analyzed  using  the  BLAST  program.  The  results  of  the research found 8 isolate of bacterias. The most unique isolate was IROD1.3. It has significant  proteolytic  activity  based  on  the  ability  to  produce  clear  zone  of protease on SMA medium with a diameter of 85,00 mm. Isolate IROD1.3 was identified  molecularly  as  Bacillus  thuringiensis  with  similarity  of  96% to  the sequence of 16S rRNA gene Bacillus thuringiensis strain TERI SID4 (Genbank access code: KX822158.1). Keywords: Protease, 16S ribosomal RNA gene, red oncom, proteolytic bacterium
Co-Authors - Yusrin Adela Kartikasari Adhimah, Dewi Rokhmatul Alfiani, Yulia Alisha Triwahyuni Andri Sukeksi Anggraeni Puspitasari Asri Rahmiati Atwiyandani, Indah Audina, Risti Ifa Aufit Fahima Aulia Harun Ayu Rahmawati Sulistyaningtyas Azizah, Inas Hasna Bagus Irawan Buchari - Budi Santosa Budi Santosa Cahyani, Agung Eka Cahyani, Anak Agung Ayu Eka Cahyaningrum, Dea Galuh Debby Permata Sari Dewi, Juliana Permata Diah Heti S Dianson, Arkila Destha Endah Mubiarti Endang Handayani Endang Prihariyanti Endang Tri Wahyuni Endang Tri Wahyuni M Endang Tri Wahyuni Maharani Endang Triwahyuni Maharani Fandhi Adi Wardoyo Faridah Faridah Febriansyah, Moch. Rizky Feri Feri Fitri Nuroini Fitria, Meutia Srikandi Hikmah, Annisa Nurul Janah, Inayah Qori Nur Jannah, Erni M Jatmiko Susilo Jimat, Resi Tondho Lusti, Nur Fara Maulida Julia Saputri Meka Zainal Farabi Meyliana, Adinda Mindhumalid, Tatut Muh. Amin Muhammad Ali Zulfikar Musaidah, Siti Noverson Lidaya Nur Azizah Nur Maesaroh Pika Nurropiah Pramesti, Novia Eka Pratama, Aji Sukma Pratiwi, Nunik Putri, Desty Ratna Radna Safitri Rinaldi, Muhamad Rizqi Rinda Aulia Utami Rino R. Mukti Sakti Imam Muchlissin Sari, Sintia Sazuana, Yumi Sinta Dwi Astuti Siska Nurprihandayani Sri Darmawati Sri Darmawati Sri Elvira Sri Riris Septianingsih Stalis Norma Ethica Sulistyaningtyas, Ayu Rahmawati Theresia Liyana Sejati Triyono Triyono Tulus Ariyadi Ubaidah, Shofura Salma Wa Ode Inayatul Wiwi Yuli Lestari Yayan Atikasari Yusrin - Yusrin Yusrin