Jurnal Biologi Tropis
Vol. 25 No. 1 (2025): Januari - Maret

DPPH Radical Capture Test of Sumbawa Oil and Identification of Compound Content Using GC-MS

Wardani, Ray Haerul (Unknown)
Permatasari, Lina (Unknown)
Hidayati, Agriana Rosmalina (Unknown)
Hanifa, Nisa Isneni (Unknown)



Article Info

Publish Date
15 Feb 2025

Abstract

Free radicals are reactive fragments that can trigger a variety of chronic diseases. Antioxidant compounds are needed to counteract the attack of free radicals. Sumbawa oil is one of the natural traditional remedies that has the potential to be a natural antioxidant because it contains compounds such as lauric acid, palmitic acid, linoleic acid, stearic acid, and methyl palmitate. The study aimed to determine the metabolite compounds contained in the Sumbawa oil brand “Rimba” and their activity to capture the DPPH free radical. The Sumbawa oil determined their metabolite compounds using Gass Chromatography-Mass Spectrometry (GC-MS). The antioxidant activity of Sumbawa oil was analyzed using the DPPH radical capture method. The GC spectra showed the 5 compounds detected in the Sumbawa oil. In addition, the MS spectra showed these 5 compounds were delta-3-carene, trans-caryophyllene, palmitic acid, palmitoleic acid, and stearic acid. The antioxidant activity of Sumbawa oil was found to be very weak with an IC50 value of 13.49 ± 0.34 mg/mL. Therefore, Palmitoleic acid and palmitic acid were reported to have potential antioxidant activity. These compounds were lipophilic antioxidants that did not properly detect their antioxidant activity using the DPPH method. So, the antioxidant activity of Sumbawa oil was recommended to be analyzed using the β-carotene bleaching method.

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Journal Info

Abbrev

JBT

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Description

Jurnal Biologi Tropis (ISSN Cetak 1411-9587 dan ISSN Online 2549-7863) diterbitkan mulai tahun 2000 dengan frekuensi 2 kali setahun oleh Program Studi Pendidikan Biologi PMIPA FKIP Universitas Mataram, berisi hasil penelitian dan ulasan Ilmiah dalam bidang Biologi ...