The purpose of this study was to determine the best dose of papain in commercial feed to increase the growth of Climbing perch (Anabas testudineus). The research container used was a black bucket with a round shape with a volume of 100 L with a stocking density of 20 fish/container or 400 fish/m3. This study used an experimental method with a completely randomized design (CRD) with one factorial, five treatments and three replications. The treatment applied was feeding containing papain enzyme with different doses, namely P0= without the addition of papain enzyme, P1= addition of papain enzyme 1.25%/kg feed, P2= addition of papain enzyme 2.25%/kg feed, P3= papain enzyme 3.25%/kg feed and P4= addition of papain enzyme 4.25%/kg feed. The results showed a significant effect (P<0.05) on absolute weight, absolute length, specific growth rate, feed conversion ratio, enzyme activity and there was no significant effect (P>0.05) on the survival rate. The best growth rate was found in treatment P3 with absolute weight value: 4.70±0.18 g, absolute length: 2.0±0.20 cm, specific growth rate: 2.21±0.02% per day, survival: 100 %, feed conversion ratio: 1.26±0.03 %, and number of enzymes: 0.0716±0.0007 IU/mL, and with the growth of the average weight of water spinach 60.08 g and the average length of water spinach 100.4 cm in P1 treatment.
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