Lansium domesticum has three varieties and one of the varieties is kokosan. These plants contain limonoids as the main constituents. Phytochemical investigation of L. domesticum cv kokosan has identified onoceranoids-type triterpenoids and tetranortriterpenoids from the seeds, fruit peels, and bark except for the root. This research described dukunolide D (1) that has been obtained from the methanol extract of the root of kokosan. Methanol root extract was fractioned by n-hexane and dichloromethane (DCM). All fractions including the crude extract were screened for phytochemical test. The DCM fraction was chromatographed in order to obtain compound 1. DART-HRMS of compound 1 showed a positive ion peak at m/z 469.1861 [M+H]+ (calculated 469.1862 corresponding for C26H29O8). The structure of 1 was fully determined by 1D (1H-NMR, 13C-NMR, and DEPT-135) and 2D NMR (COSY, HSQC, HMBC, and NOESY), and by comparison with data from literature.
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