Indonesian Journal of Pharmacy and Natural Product
Vol. 8 No. 01 (2025): Indonesian Journal of Pharmacy and Natural Product

Identifikasi Senyawa Flavonoid Ekstrak Etanol 96% Cacing Tambelo (Bactronophorus Thoracites) Menggunakan Metode KLT: Identification of Flavonoid Compounds of 96% Ethanol Extract of Tambelo Worm (Bactronophorus Thoracites) Using KLT Method

Ayu Andira (Unknown)
Jufri Ubrusun (Unknown)
Faizal Mustamin (Unknown)



Article Info

Publish Date
23 Mar 2025

Abstract

Tambelo worms (Bactronophorus thoracites) are worms that live in decaying mangrove trees. The purpose of this study was to analyze the flavonoid compound content in tambelo worms (Bactronophorus thoracites) from Tepian Village, North Kalimantan Province. This study uses descriptive observational method which includes the examination of data on the identification of secondary metabolite compounds, especially color changes in thin layer chromatography test results in the form of retention factor (RF) values. The tambelo worms used in this study have been determined at the National Research and Innovation Agency (BRIN) in December 2024. In this study, the extraction method is maceration method and the identification of flavonoid compound using Thin Layer Chromatography (TLC). The results of tambelo worm extract after spraying cyroborate reagent produced a yellow stain (RF = 0.40), and produced a bright blue fluorescent stain (RF = 0.38) when observed using UV light 366 nm. The conclusion from the results of this study is that tambelo worm extract (Bactronophorus thoracites) is positive for flavonoids. Thin Layer Chromatography test results using mobile phase n-hexan:ethyl acetate (1:3) for flavonoids.   ABSTRAK Cacing tambelo (Bactronophorus thoracites) merupakan cacing yang hidup dipohon bakau lapuk dan membusuk. Tujuan penelitian ini untuk menganalisis kandungan senyawa flavonoid pada cacing tambelo (Bactronophorus thoracites) asal Desa Tepian Provinsi Kalimantan Utara. Penelitian ini menggunakan metode observasional deskriptif yang meliputi pemeriksaan data hasil identifikasi senyawa metabolit sekunder, khususnya perubahan warna pada hasil uji kromatografi lapis tipis berupa nilai faktor retensi (RF). Cacing tambelo yang dipakai pada penelitian ini telah dideterminasi di Badan Riset dan Inovasi Nasional (BRIN) pada bulan desember 2024. Pada penelitian ini ekstraksi menggunakan metode maserasi dan pengujian senyawa flavonoid menggunakan kromatografi lapis tipis (KLT). Hasil ekstrak cacing tambelo setelah penyemprotan reagen sitroborat menghasilkan bercak noda berwarna kuning (RF= 0,40), dan menghasilkan bercak noda berflourisensi biru terang (RF= 0,38) pada saat diamati menggunakan sinar UV 366 nm. Kesimpulan dari hasil penelitian ini bahwa ekstrak cacing tambelo (Bactronophorus thoracites) positif mengandung flavonoid. Hasil uji Kromatografi Lapis Tipis menggunakan fase gerak n-heksan:etil asetat (1:3) untuk flavonoid.

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Journal Info

Abbrev

ijpnp

Publisher

Subject

Medicine & Pharmacology

Description

This journal is aimed as promoting principled approach to research on pharmacy that covers a broad range of topics engaging a good relationship in theoretical and practical. This journal covers: Pharmacology Pharmacognosy Analytical Chemistry Pharmaceutical Technology Social and Management ...