Journal of Pharmacy Tiara Bunda
Vol. 3 No. 2 (2023): Journal of Pharmacy Tiara Bunda (JPTB)

Uji Aktivitas Antibakteri Ekstrak Daun Kemangi (Ocium sanctum L) terhadap bakteri Patogen dengan metode KLT Bioautografi

Dewi, Hasna (Unknown)



Article Info

Publish Date
30 Aug 2023

Abstract

A research of the antibacterial activity test extracts of basil (Ocimum sanctum L) against pathogenic bacteria by using KLT Bioautografi. This study aims to determine the activity and basil leaf extract chemical components are on the inhibition of bacterial. Basil (Ocimum sanctum L) extracted by maceration method using ethanol 70%. Extraction then screened for antibacterial activity with levels of 1 mg / ml against microbes that Staphylococcus aureus, Streptococcus mutans, Staphylococcus epidermidis, Salmonella typhi, Bacillus subtillis, Escherichia coli, Pseudomonas aeruginaosa and vibrio sp. Tests showed that 70% ethanol extract of leaves of basil can inhibit the growth of Staphylococcus aureus, Streptococcus mutans, Salmonella typhi, Bacillus subtillis, Escherichia coli, Pseudomonas aeruginaosa and vibrio coma. Testing TLC-Bioautografi basil leaves ethanol extract using ethyl acetate eluent: n-hexane (1: 3) showed inhibition of bacteria at Rf values of 0.15, 0.31, 0.44. Identification test showed that the compounds provide antibacterial flavonoids, alkaloids and terpenoids

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Journal Info

Abbrev

jptb

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

Description

Journal of Pharmacy Tiara Bunda (JPTB) is an academic journal related to the fields of pharmacy and healthcare, published by an institution named "Tiara Bunda." JPTB serves as a platform for academics, researchers, and professionals in the field of pharmacy to share knowledge, research findings, ...