The purpose of this study was to determine the difference in stress on male inmates and female inmates at the Semarang Penitentiary. The hypothesis in this study is that there are differences in stress on male and female inmates. Stress on male prisoners is higher than female prisoners. Subjects in the study amounted to 100 respondents. This study used a cluster random sampling technique. Research data was collected using the Stress Scale on Prisoners. Data analysis was performed using the t-test technique. The results of the study showed that there was a difference in stress on male inmates and female convicts at the Semarang Penitentiary as indicated by the value of tcount = 2.868 > ttable = 1.984 with p = 0.005 (p <0.05),
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