Symbiotic: Journal of Biological Education and Science
Vol. 5 No. 1 (2024): Symbiotic: Journal of Biological Education and Science Vol. 5 No. 1 April 2024

Callus growth of red starfruit (Baccaurea angulata) leaves with the addition 2,4-D (dichlorophenoxy acetic acid) and kinetin

Zulfa Zakiah (Universitas Tanjungpura)
Murni Murni (Universitas Tanjungpura)
Masnur Turnip (Universitas Tanjungpura)



Article Info

Publish Date
27 Apr 2024

Abstract

One of the obstacles to propagating red starfruit (Baccaurea angulata) using seeds is that fruit without seeds is often found. Tissue culture is an alternative for propagating red star fruit plants to provide seeds or secondary metabolites through callus culture. The research aims to determine the effect of 2,4-D and kinetin on callus growth of star fruit leaves and to obtain the best concentration to induce callus. This research used a factorial Completely Randomized Design (CRD) with two treatment factors, namely 2,4-D (0; 0.5; 1.0; 1.5; 2.0; 2.5 ppm) and kinetin (0; 0.5; 1.0; 1.5; 2.0; 2.5 ppm). The observation parameters consisted of callus emergence time (days after planting), wet and dry weight of the callus (g), color and texture of the callus. The results showed that the combination of 2,4-D and kinetin had a significant effect to callus emergence time, but didn't on the wet and dry weight of the callus. The fastest callus emergence time was obtained of 2.5 ppm 2,4-D + 0.5 ppm kinetin, 1.5 ppm 2,4-D + 2.5 ppm kinetin namely 6 DAP. The callus color is white, brown, and brown with a friable and compact texture. Another response that emerged was root growth.

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Journal Info

Abbrev

symbiotic

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Other

Description

The Journal invites original research articles and not simultaneously submitted to another journal or conference. The whole spectrum of research in Biology Education and Science are welcome, which includes, but is not limited to the following topics: Focus and Scope in Biological Education: - ...