Journal of Multidisciplinary Applied Natural Science
Vol. 5 No. 2 (2025): Journal of Multidisciplinary Applied Natural Science

Bioremediation of Profenofos-Contaminated Soil using Bio-Slurry, Exogenous, and Indigenous Microorganism Formulation from Puntukdoro Farmland, Indonesia

Pujiati, Pujiati (Unknown)
Sholikhah, Oktaviariesta Habibatus (Unknown)
Utami, Sri (Unknown)
Fatimah, Fatimah (Unknown)
Ramadhan, Rico (Unknown)
Ni'matuzahroh, Ni'matuzahroh (Unknown)



Article Info

Publish Date
28 May 2025

Abstract

Pesticide residues in soil present significant environmental and health risks, especially in regions with heavy organophosphate used in vegetable farming. This study examines bioaugmentation, an eco-friendly method for degrading soil pesticide residues, utilizing both indigenous and exogenous microorganisms, as well as bio-slurry from biogas production. Puntukdoro Village, Magetan, Indonesia, generates a substantial quantity of bio-slurry waste, which presents a promising solution to local agricultural challenges, including low crop yields and soil degradation. Puntukdoro Village produces a significant amount of bio-slurry waste, which offers a promising solution to local agricultural issues, including poor crop yield and soil degradation. The study aims to identify and formulate microorganisms from Puntukdoro using bio-slurry and exogenous cellulolytic mold formulations. This involves extracting and characterizing indigenous bacteria, preparing external supplements, and conducting ex situ bioaugmentation with seven different treatments. Ten mold isolates, including Penicillium, Monilia, Aspergillus, and Trichoderma, and eight bacterial isolates, including Micrococcus, Pseudomonas, and Bacillus, were identified. Bioremediation assays showed that both indigenous and exogenous microorganisms improved soil quality and reduced pesticide levels. The most effective treatment, P7, with 10% bio-slurry, 10% biofostik, and 10% indigenous microorganisms applied for 28 d (W4), reduced profenofos from 4.718 to 0.000 mg/kg. In contrast, treatment P2W1, with 30% biofostik for 7 d, reduced profenofos by 0.293 mg/kg. These findings indicate that exogenous and indigenous microorganisms can effectively enhance profenofos bioremediation.

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Journal Info

Abbrev

jmans

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics

Description

Journal of Multidisciplinary Applied Natural Science (abbreviated as J. Multidiscip. Appl. Nat. Sci.) is a double-blind peer-reviewed journal for multidisciplinary research activity on natural sciences and their application on daily life. This journal aims to make significant contributions to ...