This study aimed to determine the effect of the use of Technological Knowledge-based learning media on students' scientific literacy skills on the excretion system material in class XI biology subjects at SMA Negeri 5 Samarinda. The type of research used was experimental research with quantitative methods in the form of a quasi-experimental design type of the pretest-posttest control group design. The results of students' scientific literacy skills obtained the results of the N-Gain test, which is 0.59 with a moderate category, and the results of the t-test with a significance value of 0.000 <0.05. Based on these results, it was found that the use of Technological Knowledge-based learning media has a significant effect on students' scientific literacy skills on the excretion system material in class XI of SMA Negeri 5 Samarinda.
                        
                        
                        
                        
                            
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