This study aims to develop a Student Worksheet (LKPD) based on scientific literacy to improve junior high school students' scientific literacy in ecosystem material. The methodology adopted in this study is research and development (RD), using a 4D development model that includes the stages of definition, design, development, and dissemination. The trial results showed that 80% of students were more interested and motivated when learning using LKPD and students' understanding of ecosystem material increased by 75%. The developed LKPD obtained a media validity of 85.80%, a language validity of 85.71%, and a material validity of 92.475%, indicating that the LKPD is suitable for use in learning. This LKPD is designed to encourage students to think critically through scientific literacy-based questions, which are in accordance with the needs of 21st century education. The ecosystem material taught through LKPD not only provides knowledge but also shapes students' attitudes. This study makes a positive contribution to biology education in Indonesia, preparing students for future scientific and technological challenges. It is hoped that the development of LKPD based on scientific literacy can be expanded to include various other scientific materials and utilize digital technology to create more interactive and interesting learning.
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