JSFK (Jurnal Sains Farmasi & Klinis)
Vol 12 No 1 (2025): J Sains Farm Klin 12(1), April 2025

Determination of Total Phenolic Content and Antioxidant Activity of Epigallocatechin Gallate Rich Fraction from Tea Leaves (Camellia sinensis L.)

Amelia, Puteri (Unknown)
Citraningsih, Kinanti (Unknown)
Dewi, Asri Kusuma (Unknown)
Anggia, Vivi (Unknown)
Anggraeni, Yuni (Unknown)



Article Info

Publish Date
01 Jul 2025

Abstract

The tea plant, Camellia sinensis L., is a well-known natural product with significant health advantages. The main catechin found in green tea leaves is epigallocatechin gallate (EGCG), which has potent antioxidant properties. EGCG is a common active ingredient in products from the medical and cosmetics industries. This study examines the antioxidant properties and total phenolic content (TPC) of an EGCG-rich fraction (ERF) that is extracted from green tea leaves. TPC was quantified using the Folin-Ciocalteu method, with absorbance measured via UV-VIS spectrophotometry at 760.5 nm. Antioxidant activity was assessed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, with absorbance recorded at 516 nm. The findings indicated that the TPC of ERF was 437.18 mg GAE/g ± 5.54, while the IC50 value for antioxidant activity was 4.47 mg/L, This value was lower than that of the positive control, Vitamin C, which exhibited an IC₅₀ of 5.07 mg/L. These results suggest that ERF could be developed into a natural ingredient for cosmetics and dietary supplements.

Copyrights © 2025






Journal Info

Abbrev

jsfk

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

Description

Jurnal Sains Farmasi & Klinis (J Sains Farm Klin) adalah jurnal ilmiah di bidang kefarmasian yang diterbitkan oleh Fakultas Farmasi Universitas Andalas bekerjasama dengan lembaga profesi "Ikatan Apoteker Indonesia" (IAI) daerah Sumatera Barat sejak tahun ...