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All Journal JSFK (Jurnal Sains Farmasi & Klinis)
Dewi, Asri Kusuma
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Determination of Total Phenolic Content and Antioxidant Activity of Epigallocatechin Gallate Rich Fraction from Tea Leaves (Camellia sinensis L.) Amelia, Puteri; Citraningsih, Kinanti; Dewi, Asri Kusuma; Anggia, Vivi; Anggraeni, Yuni
JSFK (Jurnal Sains Farmasi & Klinis) Vol 12 No 1 (2025): J Sains Farm Klin 12(1), April 2025
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.12.1.36-40.2025

Abstract

The tea plant, Camellia sinensis L., is a well-known natural product with significant health advantages. The main catechin found in green tea leaves is epigallocatechin gallate (EGCG), which has potent antioxidant properties. EGCG is a common active ingredient in products from the medical and cosmetics industries. This study examines the antioxidant properties and total phenolic content (TPC) of an EGCG-rich fraction (ERF) that is extracted from green tea leaves. TPC was quantified using the Folin-Ciocalteu method, with absorbance measured via UV-VIS spectrophotometry at 760.5 nm. Antioxidant activity was assessed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, with absorbance recorded at 516 nm. The findings indicated that the TPC of ERF was 437.18 mg GAE/g ± 5.54, while the IC50 value for antioxidant activity was 4.47 mg/L, This value was lower than that of the positive control, Vitamin C, which exhibited an IC₅₀ of 5.07 mg/L. These results suggest that ERF could be developed into a natural ingredient for cosmetics and dietary supplements.
Co-Authors Amelia, Puteri Citraningsih, Kinanti Vivi Anggia, Vivi Yuni Anggraeni