Human life on earth cannot be separated from water. Water is an absolute necessity that humans need. Water needs can be obtained from 2 sources, namely water from the surface and water from the ground. Springs are a source of water obtained from groundwater. Kalisegoro 2 spring is one of the springs in Gunung Pati District, Semarang City. This spring is in Kalisegoro Village where the surrounding land cover is vegetation. This type of research is quantitative in the form of analysis of species diversity and tree distribution patterns. The aim of this research is to determine the diversity of tree species and tree distribution patterns in Kalisegoro 2 springs in Gunung Pati District. The results of this research show that the diversity of vegetation in the Kalisegoro 2 springs amounts to 145 individuals and 10 types of species, the Swietenia mahagoni species is the dominant species found at the Kalisegoro 2 springs location with an INP of 0.59. The vegetation diversity index at Kalisegoro 2 springs is included in the medium category in the range 1
                        
                        
                        
                        
                            
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