<![CDATA[Scientific explanation is an essential skill in chemistry learning because it connects scientific principles and objective evidence. This study aims to describe the application of guided inquiry-based learning in thin-layer chromatography experiments of synthetic and natural food coloring to develop students' scientific explanations in learning applications. This study used a pre-experimental method with a post-test only one-shot case study design. In the implementation of learning, the implementation rate reached 87%. The average scores for worksheets and scientific explanation tests were 78 and 79, respectively. This indicates that the application of guided inquiry-based worksheets through TLC experiments is practical in developing students' scientific explanation skills. This experiment can be adopted in broader chemistry learning contexts by integrating guided inquiry with thin-layer chromatography using real food colorants, thereby fostering scientific thinking and providing students with hands-on experience that emphasizes the relevance of chemistry in everyday life.]]>
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