Jurnal Agrotropika
Vol. 24 No. 1 (2025): Jurnal Agrotropika Vol 24 No 1, Mei 2025

SCALP AND SHOOT FORMATION IN RESPONSE TO THIDIAZURON CONCENTRATIONS IN VITRO CULTURE OF PLANTAIN ‘TANDUK’

Hapsoro, Dwi (Unknown)
Prayogi, Adi Noor (Unknown)
Ramadiana, Sri (Unknown)
Yusnita, Yusnita (Unknown)



Article Info

Publish Date
01 May 2025

Abstract

This experiment aimed to study the formation of scalps and  banana shoots 'Tanduk' in vitro in response to thidiazuron (TDZ). Shoot explants measuring ± (1 x 1 x 1) cm3 were cultured in precondition media (MS+ 5 mg/l benzyladenine) for 4 weeks, then transferred to treatment media, namely MS + TDZ. This experiment was carried out using a completely randomized design with 3 replications and 7 treatments of TDZ concentrations (0.5; 1.0; 1.5; 2; 2.5; 3; 3.5; 4.0 mg/l).  Each experimental unit consisted of 5 bottles, each containing one explant. Observations of scalp number were carried out at 8 weeks of age. The scalps were subcultured on MS+5mg/l BA media to induce shoots. After 8 weeks in the shoot induction medium, the number of shoots was recorded.  The results showed that at 8 weeks on the culture all of the explants formed scalps, with the highest number of scalps (6 scalps/explant) was at 1 mg/l TDZ.  The scalp number decreased to 4.4 to 3.5/explant as the TDZ concentration increased from 1.5 to 4 mg/l. After 8 weeks on shoot induction media containing 5 mg/l BA, the highest number of shoots (3.8 shoots/explant) was obtained on the scalp from the 0.5 mg/l TDZ treatment. The shoot number decreased to (3.2-1.9/explant) with increasing TDZ concentrations from 1.0 to 4.0 mg/l.Keywords: Tanduk, plantain, thidiazuron, in vitro, scalp.

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Journal Info

Abbrev

JAT

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

Scientific article dealing with broad aspects of tropical agronomy including crop production, horticulture, plant pests and diseases, post-harvest, sustainable agriculture, soil science, climatology, ecology, biodiversity, and ...