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SCALP AND SHOOT FORMATION IN RESPONSE TO THIDIAZURON CONCENTRATIONS IN VITRO CULTURE OF PLANTAIN ‘TANDUK’ Hapsoro, Dwi; Prayogi, Adi Noor; Ramadiana, Sri; Yusnita, Yusnita
JURNAL AGROTROPIKA Vol. 24 No. 1 (2025): Jurnal Agrotropika Vol 24 No 1, Mei 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i1.9927

This experiment aimed to study the formation of scalps and banana shoots 'Tanduk' in vitro in response to thidiazuron (TDZ). Shoot explants measuring ± (1 x 1 x 1) cm3 were cultured in precondition media (MS+ 5 mg/l benzyladenine) for 4 weeks, then transferred to treatment media, namely MS + TDZ. This experiment was carried out using a completely randomized design with 3 replications and 7 treatments of TDZ concentrations (0.5; 1.0; 1.5; 2; 2.5; 3; 3.5; 4.0 mg/l). Each experimental unit consisted of 5 bottles, each containing one explant. Observations of scalp number were carried out at 8 weeks of age. The scalps were subcultured on MS+5mg/l BA media to induce shoots. After 8 weeks in the shoot induction medium, the number of shoots was recorded. The results showed that at 8 weeks on the culture all of the explants formed scalps, with the highest number of scalps (6 scalps/explant) was at 1 mg/l TDZ. The scalp number decreased to 4.4 to 3.5/explant as the TDZ concentration increased from 1.5 to 4 mg/l. After 8 weeks on shoot induction media containing 5 mg/l BA, the highest number of shoots (3.8 shoots/explant) was obtained on the scalp from the 0.5 mg/l TDZ treatment. The shoot number decreased to (3.2-1.9/explant) with increasing TDZ concentrations from 1.0 to 4.0 mg/l.Keywords: Tanduk, plantain, thidiazuron, in vitro, scalp.

Successful Grafting of Two Indonesian Clones of Piper nigrum L. with P. colubrinum Link.: Effects of IBA and NAA on Rooting and Effects of BA on Grafting Yusnita, Yusnita; Hapsoro, Dwi; Prayogi, Adi Noor; Agustiansyah, Agustiansyah; Karyanto, Agus
AGRIVITA Journal of Agricultural Science Vol 46, No 1 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i1.3899

This study aimed to investigate the effects of auxin types and their concentrations on rooting of the rootstock cuttings and BA on grafting. First, IBA, NAA or IBA+NAA, each at 0, 1000, 2000, and 3000 ppm were used as treatments to study rooting of Piper colubrinum. In the second experiment, BA (0 and 50 ppm) was applied to P. nigrum scion clones of Natar-1 and Petaling-2, before being grafted to the rootstocks. Results showed, that all types of auxins (IBA, NAA or IBA+NAA) induced rooting and shoot growth, and the increase of auxin concentrations led to the increase of rooting. However, their effectiveness was different. NAA and IBA+NAA were superior to IBA, and at 2000 ppm, IBA+NAA resulted in the best plant growth, as indicated by higher values of both rooting and shoot growth parameters. Compared to control, BA treatment on scions resulted in higher grafting success (80% vs. 93%) for Natar-1, and (73% vs.100%) for Petaling-2 clones. BA treatment on scions also induced more calluses in the graft union and better shoot growth. To our knowledge, this is the first report on the use of BA to increase success of grafting between P. nigrum and P. colubrinum.

Enhancing Vigor of Cucumber (Cucumis sativus L.) Seeds Through Osmo-priming Pradana, Adhitya Vishnu; Anugrah, Mertya; A'yunin, Qurrotu; Prayogi, Adi Noor; Togatorop, Eny Rolenti
Jurnal Ilmu Pertanian Vol 10, No 3 (2025): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.111401

Cucumber production relies heavily on high-quality seeds, yet prolonged storage often leads to a significant decline in seed vigor and viability, characterized by reduced germination and non-uniform growth. To address this challenge, invigoration techniques through seed priming offer a promising solution to revitalize aged seeds, which also aligns with global sustainability efforts in preserving genetic resources and ensuring food security. This study aimed to evaluate the effectiveness of various invigoration treatments in enhancing the viability and vigor of expired cucumber seeds from different storage periods. The research utilized a factorial split-plot design with two factors, consisting of invigoration treatments (Control, KH₂PO₄, PEG, and Distilled Water) and storage periods (9 years, 7 years, and 5 years). The variables observed included radicle emergence (RE) at 24 and 48 hours, germination capacity, percentage of abnormal seedlings, and vigor index. The results showed that all invigoration treatments significantly improved seed performance compared to the control. The KH₂PO₄ treatment was the most effective for rapid improvement within 24 hours, showing a strong correlation (R=0.99) with increased vigor and a reduction in abnormal seedlings (R=0.85). However, distilled water showed the best performance for 48-hour RE, particularly when applied to 5-year stored seeds, reaching an 88% germination rate and the highest vigor index of 80%. In conclusion, invigoration is a viable method to restore the quality of aged seeds, with KH₂PO₄ and distilled water being recommended for optimizing the performance of specific seed lots. Future studies could further explore the field establishment of these invigorated seeds under diverse environmental stress conditions.

Enhancing Vigor of Cucumber (Cucumis sativus L.) Seeds Through Osmo-priming Pradana, Adhitya Vishnu; Anugrah, Mertya; A'yunin, Qurrotu; Prayogi, Adi Noor; Togatorop, Eny Rolenti
Jurnal Ilmu Pertanian Vol 10, No 3 (2025): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.111401

Cucumber production relies heavily on high-quality seeds, yet prolonged storage often leads to a significant decline in seed vigor and viability, characterized by reduced germination and non-uniform growth. To address this challenge, invigoration techniques through seed priming offer a promising solution to revitalize aged seeds, which also aligns with global sustainability efforts in preserving genetic resources and ensuring food security. This study aimed to evaluate the effectiveness of various invigoration treatments in enhancing the viability and vigor of expired cucumber seeds from different storage periods. The research utilized a factorial split-plot design with two factors, consisting of invigoration treatments (Control, KH₂PO₄, PEG, and Distilled Water) and storage periods (9 years, 7 years, and 5 years). The variables observed included radicle emergence (RE) at 24 and 48 hours, germination capacity, percentage of abnormal seedlings, and vigor index. The results showed that all invigoration treatments significantly improved seed performance compared to the control. The KH₂PO₄ treatment was the most effective for rapid improvement within 24 hours, showing a strong correlation (R=0.99) with increased vigor and a reduction in abnormal seedlings (R=0.85). However, distilled water showed the best performance for 48-hour RE, particularly when applied to 5-year stored seeds, reaching an 88% germination rate and the highest vigor index of 80%. In conclusion, invigoration is a viable method to restore the quality of aged seeds, with KH₂PO₄ and distilled water being recommended for optimizing the performance of specific seed lots. Future studies could further explore the field establishment of these invigorated seeds under diverse environmental stress conditions.

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