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IN VITRO SHOOT REGENERATION OF INDONESIAN BANANAS (Musa spp.) cv. AMBON KUNING AND RAJA BULU, PLANTLET ACCLIMATIZATIONAND FIELD PERFORMANCE Yusnita, Yusnita; Danial, Ekawati; Hapsoro, Dwi
AGRIVITA, Journal of Agricultural Science Vol 37, No 1 (2015): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v37i1.438

The aim of this study was to observe responses of two banana cultivars ‘Ambon’ and ‘Raja Bulu’ on different BA concentrations and effects of different media and fertilizer on survival and growth of plantlets. Sterilized explants were cultured on initiation medium (MS with 1mg L-1 BA) for 4 weeks, then subjected to media MS with 2.5, 5.0, or 7.5 mg L-1 BA. Numbers of shoot buds, shoots and propagules were recorded after 4 consecutive passages with 4 weeks intervals. Rooted plantlets were acclimatized in three different media, then treated with or without NPK (32:10:10) fertilizer solution once a week. After 2 months, the survival and growth of plantlets were recorded. Cultures of banana ‘Ambon Kuning’ showed higher regenerative capacity compared to ‘Raja Bulu’, producing higher numbers of shoot buds, shoots and propagules. The best medium for propagule proliferation of both banana cultivars was MS+5 mg L-1 BA, producing 40.7 propagules for ‘Ambon Kuning’, and 12.3 propagules for ‘Raja Bulu’ per explant. In all acclimatization media tested, 100% of plantlet survival was achieved. The best plantlet growth was found in sand: compost (1:1,v/v) with application of NPK solutions. The in vitro-derived plants were planted in the field and produced fruits of high quality.

Resistensi Kacang Tanah Transgenik yang Membawa Gen cp PStV Stabil Sampai Tujuh Generasi Silang-Dalam terhadap Peanut Stripe Virus Hapsoro, Dwi; Aswidinnoor, Hajrial; Jumanto, Jumanto; Suseno, Rusmilah; Sudarsono, Sudarsono
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

Penyakit bilur kacang tanah yang disebabkan oleh peanut stripe virus (PStV)merupakan salah satu penyakit utama pada pertanaman kacang tanah (Arachishypogaea L.). Penggunaan varietas tahan merupakan alternatif paling efektif untukmengatasi penyakit tersebut. Rekayasa genetika merupakan metode efektif untukmendapatkan varietas kacang tanah yang resisten PStV. Tujuan penelitian ini adalahuntuk (1) mengetahui respons tanaman kacang tanah transgenik yang membawa gen cpPStV terhadap infeksi PStV dan (2) menguji stabilitas transgen sampai tujuh generasisilang-dalam. Tanaman kacang tanah transgenik cv. Gajah generasi T0, T1, T2, T3, T5,T6, dan T7 diinokulasi secara mekanik dengan PStV. Terdapat tiga jenis respons kacangtanah transgenik terhadap infeksi PStV, yaitu resisten, recovery, dan rentan. Padatanaman resisten gejala tidak muncul. Pada tanaman recovery, gejala chlorotic ringmottle muncul pada satu daun atau lebih, selanjutnya gejala tidak tampak pada daundaunyang tumbuh kemudian. Pada tanaman rentan, gejala severe blotch muncul padasuatu daun, selanjutnya gejala tersebut tetap muncul pada seluruh daun yang tumbuhkemudian. Transgen cp PStV tetap stabil setelah mengalami tujuh generasi silangdalam.Sejumlah galur murni kacang tanah transgenik yang resisten PStV telahdiidentifikasi.

RESPON PERTUMBUHAN TUNAS DARI PROTOCORM-LIKE BODIES MENJADI PLANLET ANGGREK DENDROBIUM HIBRIDA IN VITRO TERHADAP DUA JENIS MEDIA DAN PEMBERIAN TRIPTON Zasari, Maera; Ramadiana, Sri; Yusnita, Yusnita; Hapsoro, Dwi
JURNAL AGROTROPIKA Vol 15, No 1 (2010): Agrotropika Vol.15 No.1 2010
Publisher : JURNAL AGROTROPIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (258.501 KB)

Dendrobium is great interest because of its high value as a floricultural commodity and a kind of elite herbage plant in Indonesia. An efficient in vitro propagation system via protocorm-like body (PLBs) induction has been developed using one-year-old axillary node explants. The potential of different media and supplements triptone for reliable Dendrobium orchid plantlet regeneration from in vitro PLBs explants were studied. In all two media under investigation, shoot regeneration was achieved when the PLBs were transferred onto solidified MS half strenght and Growmore medium with or without 2 g L-1 triptone supplemented with 15% (v/v) coconut water, 20 g L-1 sucrose, and 2 g L-1 charcoal. The shoots development investigation after three month of culture. 1/2 MS and Growmore media significantly influenced the plantlet regeneration of Dendrobium orchid. Among the media, Growmore showed better performance on shoots and leaves per plantlet, but triptone showed no significant effect on number shoots and leaves. The longest shoots were found in Growmore medium without triptone. Key words: Dendrobium, in vitro, PLBs, planlet, triptone.

KAJIAN TEKNIK PERBANYAKAN VEGETATIF PISANG AMBON KUNING DENGAN PEMBELAHAN BONGGOL (CORM) Rugayah, Rugayah; Hapsoro, Dwi; Ulumudin, Ade; Motiq, Feria Wirana
JURNAL AGROTROPIKA Vol 17, No 2 (2012): Agrotropika Vol.17 No.2 2012
Publisher : JURNAL AGROTROPIKA

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Propagation by corm division of banana provides an opportunity to get the seedling in large quantities, but need technology to promote the growth of spur on the buds that are still dormant. Research conducted in 2010 in the greenhouse of Faculty of Agriculture, University of Lampung aimed to determine the effect of growing media and concentration of BA in Yellow banana?s Ambon multiplication by corm division. The study con-sisted of 2 subtitle designed in perfectly with the design of randomized groups factorial (4x2) which is repeated 3 times. The first factor is the same in both experiments: BA concentrations (0, 50, 100, 200 mg / l) and the second factor in the first experiment: the type of media (sand mixed with rice husk and sand with compost 1:1) and in Experiment II: cleavage divition (four and eight parts). Planting material in Experiment I, hump into four sections and planting medium used in Experiment II, a mixture of sand and rice husk 1:1. The variables measured were: time emerged shoots, number of shoots, and shoot height, diameter of the base of shoots, number of leaves, leaf width, number of roots, and the average length of the roots. Data were analyzed by using a variety of software Mini Tab, followed by a test of least significant difference (LSD) 5%. The results showed that: (1) growth of shoots on a mixture of sand and compost media better than husk fuel mixture, as well as in treatment 4 parts division hump better than 8 parts. But seen the efficiency of shoot growth, division 8 section bits more efficient because it produces more shoots (4.8 shoots) without giving BA, whereas if split 4 parts need BA a concentration of 50 ppm. Giving BA 50 ppm in sand and compost to increase the number of shoots indicated for the highest percentage (91.67%). Keywords: Yellow banana?s Ambon, corm divition, benzyladenine, growing media.

PENGARUH BERBAGAI JENIS AUKSIN DENGAN BEBERAPA KONSENTRASI TERHADAP PENGAKARAN SETEK MELADA (Piper colubrinum) Hanum, Farida; Yusnita, Yusnita; Hapsoro, Dwi; Agustiansyah, Agustiansyah; Karyanto, Agus
Jurnal Agrotek Tropika Vol 10, No 1 (2022): JURNAL AGROTEK TROPIKA VOL 10, JANUARI 2022
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v10i1.5253

Penelitian ini bertujuan untuk mengetahui pengaruh berbagai jenis auksin terhadap beberapa konsentrasi terhadap pengakaran Piper colubrinum. Percobaan dilaksanakan di Rumah Kaca Fakultas Pertanian Universitas Lampung dari bulan Mei 2018 sampai Desember 2018. Percobaan menggunakan rancangan acak lengkap dengan 3 ulangan. perlakuan yang mengaplikasikan: kontrol, IBA 1500 ppm, IBA 2000 ppm, NAA 2000 ppm, NAA 750 ppm + IBA 750 ppm, NAA 1000 ppm + IBA 1000 ppm. Hasil penelitian menunjukkan bahwa pada variabel jumlah akar primer di buku pada perlakuan NAA 750 ppm + IBA 750 ppm nilai rata-rata meningkat yaitu 21 helai dibandingkan kontrol 2,5 helai. Pada perlakuan NAA 750 ppm + IBA 750 ppm berpengaruh pada peningkatan jumlah akar primer di penampang batang yaitu 14 dibandingkan dengan kontrol 11 helai. Demikian perlakuan NAA 750 ppm + IBA 750 ppm berpengaruh pada peningkatan jumlah akar primer yaitu 34,3 helai dibandingkan dengan kontrol 13,5 helai. Untuk variabel bobot basah akar nilai rata-rata NAA 750 ppm + IBA 750 ppm yaitu 11,8 g, nilai rata-rata ini lebih tinggi dibandingkan semua perlakuan.

Respons Pembungaan Anggrek Vanda Douglas terhadap Pemupukan KNO3, Aplikasi Sitokinin (Benziladenin dan Thidiazuron), serta Pemotongan Pucuk : Vanda Douglas Suri, Rindang Andam; Yusnita, Yusnita; Hapsoro, Dwi
Jurnal Penelitian Pertanian Terapan Vol 25 No 1 (2025)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v25i1.3435

Vanda Douglas orchid is the one of tropical famous plant especially in Indonesia. The beauty of Vanda Douglas orchid is attract people’s attention as an artifial plant, cutting flower, garden decoration and public facility. The aim of research to learn more about : KNO3 fertilization, cytokinin ZPT application which is using Benzyladenine (BA) with or without Thidiazuron (TDZ), detect if there is an interaction or no interaction between application of KNO3 and ZPT cytokinin, also shoot decapitation on the flowering of Vanda Douglas orchids. Research was held in Sanggar Bunga Douglas Kelurahan Sumur Putri, Kecamatan Teluk Betung Utara, Kota Bandar Lampung started from November 2021 to February 2022. First attempt did using a Group Random Design (RAK) with three repetation. The treatment was arranged using factorial 2x5 and the first factor was KNO3 which was K0 (no fertilizer) and K1 (10 g/l of KNO3), and second factor were variant concentrate of BA and TDZ (mg/l) : no ZPT as a control, 200 BA, 400 BA, 200BA+15 TDZ and 400 BA+15 TDZ. Each treatment combination was repeating for three times and every single research consisted of 10 Vanda Douglas orchids and the number of whole plants reach 300 of Vanda Douglas orchids. Second attempt was a factorial research with Group Random Design (RAK) consisted of 2 factor. The first factor was no shoot decapitation (P0) and with shoot decapitation (P1). Second factor were variant concentrate of BA : 0, 200, 400, 200 mg/l + 15 mg/l TDZ, and 400 mg/l + 15 mg/l TDZ. Each treatment combination was repeating for three times and every single research consisted of 10 Vanda Douglas orchids and the number of whole plants reach 300 of Vanda Douglas orchids. Data analyze did by using variant analyze and if showed the significancy, it would continued by BNT test on 5% level. Observation of first attempt started on second week after KNO3 and ZPT sprayed. Observation of second attempt started in week 24 ( or week 12 after shoot decapitation). Variable that observed were : percentage of flowery plant (%) grain length (cm) and number of bud. The result of research showed that combination between KNO3 fertilization and ZPT gave the tiny effect to Vanda Douglas orchid flowering. Application of ZPT (BA or BA+TDZ), shoot decapitation and interaction between both factors was effecting to flowery plant percentage. Only for average of grain length variable and number of bud, the variant analyze did for single factor which was ZPT application. It was caused by no shoot decapitation treatment the whole plants (120 plants) wasn’t flowery. The variant analyze showed that ZPT application had real effect to grain length and bud.

In Vitro Growth and Development of Progenies Derived from Crossings of Several Dendrobium Accessions Under Different Media Maulida, Desi; Yusnita, Yusnita; Hapsoro, Dwi; Agustiansyah, Agustiansyah; Karyanto, Agus; Hamiranti, Rahmadyah; Nugroho, Rizki Bagus
AGRIVITA Journal of Agricultural Science Vol 47, No 2 (2025)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v47i2.4505

The objective of plant hybridization is to generate alternative superior phenotypes with the aim of decreasing the dependence on wild orchid exploitation in nature. Although interspecies crosses in Dendrobium are very likely to occur and there is a low chance of experiencing bud depression, interspecies crosses have the potential to encounter several problems, including failure of embryo development resulted to the seeds unable to germinate. Media formulation is the most essential component in the success of in vitro culture of orchid seeds. Therefore, this study aimed to obtain new Dendrobium hybrids and the best media formulation for germination and seedling growth. A total of three experiments were conducted including hybridization, the impacts of basic media namely (1) MS, (2) VW, and (3) foliar fertilizer both with and without the addition of tryptone, on seedling germination. The results showed that there was no compatibility barriers observed in all crosses. Foliar fertilizer + tryptone media was recommended for orchid seedlings because it produced better growth and roots up to 8 months after planting (MAP). For best results on Dendrobium hybrid seedlings, MS + tryptone media up to 6 MAP was used followed by deflasking into VW + tryptone to stimulate optimal root growth before hardening.

SCALP AND SHOOT FORMATION IN RESPONSE TO THIDIAZURON CONCENTRATIONS IN VITRO CULTURE OF PLANTAIN ‘TANDUK’ Hapsoro, Dwi; Prayogi, Adi Noor; Ramadiana, Sri; Yusnita, Yusnita
JURNAL AGROTROPIKA Vol. 24 No. 1 (2025): Jurnal Agrotropika Vol 24 No 1, Mei 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i1.9927

This experiment aimed to study the formation of scalps and banana shoots 'Tanduk' in vitro in response to thidiazuron (TDZ). Shoot explants measuring ± (1 x 1 x 1) cm3 were cultured in precondition media (MS+ 5 mg/l benzyladenine) for 4 weeks, then transferred to treatment media, namely MS + TDZ. This experiment was carried out using a completely randomized design with 3 replications and 7 treatments of TDZ concentrations (0.5; 1.0; 1.5; 2; 2.5; 3; 3.5; 4.0 mg/l). Each experimental unit consisted of 5 bottles, each containing one explant. Observations of scalp number were carried out at 8 weeks of age. The scalps were subcultured on MS+5mg/l BA media to induce shoots. After 8 weeks in the shoot induction medium, the number of shoots was recorded. The results showed that at 8 weeks on the culture all of the explants formed scalps, with the highest number of scalps (6 scalps/explant) was at 1 mg/l TDZ. The scalp number decreased to 4.4 to 3.5/explant as the TDZ concentration increased from 1.5 to 4 mg/l. After 8 weeks on shoot induction media containing 5 mg/l BA, the highest number of shoots (3.8 shoots/explant) was obtained on the scalp from the 0.5 mg/l TDZ treatment. The shoot number decreased to (3.2-1.9/explant) with increasing TDZ concentrations from 1.0 to 4.0 mg/l.Keywords: Tanduk, plantain, thidiazuron, in vitro, scalp.

Effect of long-term Tillage and Nitrogen Fertilization Residue on Soil Biochemical Properties and Cowpea Yield Afriliyanti, Rizki; Yusnaini, Sri; Karyanto, Agus; Hapsoro, Dwi; Niswati, Ainin; Utomo, Muhajir
JOURNAL OF TROPICAL SOILS Vol. 26 No. 3: September 2021
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2021.v26i3.141-147

Sustainable soil management practices must enhance or maintain soil quality and crop yields. The objective of this experiment was to study the effect of long-term (32 years) tillage system and nitrogen fertilization residue on soil biochemical properties and cowpea (Vigna unguiculata) yields. This research was conducted using four replications, a factorial experiment arranged in a randomized block design. The first factor was tillage systems consisting of intensive tillage (T1) and no-tillage (T2) and the second factor was nitrogen fertilization residue (N) consisting of N1 (0 kg N ha-1) and N2 (200 kg N ha-1). Data were analyzed using analysis of variance; if there was a significant difference will be continued with the Least Significant Difference (LSD) test at 5%. Principal Component Analysis (PCA) determined the relationship among observed variables. The results showed that no-tillage could give better results on soil respiration and soil bacteria and fungi population than intensive tillage. Lower soil pH was found at 200 kg N ha-1 fertilizer residue than control (without N fertilizer). The cowpea growth and yields were high at long-term N1T2 and N2T1 treatment. Principal component analysis showed an interrelationship among soil biochemical properties, growth, and yield of cowpea.

RESPON PERTUMBUHAN DAN HASIL PADI VARIETAS IR NUTRIZINC TERHADAP APLIKASI BIOCHAR DAN BENZILADENIN Rafika, Eni; Yusnita, Yusnita; Karyanto, Agus; Hapsoro, Dwi; Pramono, Eko
JURNAL AGROTROPIKA Vol. 24 No. 2 (2025): Jurnal Agrotropika Vol 24 No 2, Oktober 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i2.10895

Rice growth and yield can be increased through various efforts, including the use of "high-yielding varieties, fertilization, soil conditioners with application of plant growth regulators (PGRs). Soil conditioners namely as biochar are carbon products that can enhance plant productivity through enhancing the physical, chemical, and biological characteristics of the soil, while cytokinine benzyladenine (BA) is a PGR that has been widely documented to stimulate cell growth, cell division, and differentiation in rice plants. The purpose of this study was to determine the effect of biochar and benzyladenine (BA) on the growth and yield of the IR Nutrizinc rice variety. Data collection was carried out in October 2024 - February 2025 in Kampung Payung Rejo, Pubian District, Central Lampung Regency. The treatments were arranged factorially (2x3) using a completely randomized design with 3 replications. Factor I was without biochar and given 20 tons/ha of biochar. The second factor was 3 benzladenin (BA) concentrations, namely 0 ppm, 50 ppm, and 100 ppm applied by spraying on the surface of the plant at 20, 30, and 40-days after transplanting, while the biochar application was implemented 2 weeks before transplanting. Observed variables included plant height, maximum tillers per clump, count of productive tillers, length of panicles, and grains per panicle, percentage of empty grains per panicle, dry harvested grain yield, dry harvest grain yield, 1000 grain weight and dry weight of the crownThe Bartlett test was employed to assess the homogeneity of variance among treatments then analysis of variance was carried outIf a notable difference existed between treatments, the BNT test was carried out at the 5% significance level. The findings indicated that applying 20 tons/ha of biochar enhanced the yield components in terms of dry harvested grain, dry milled grain, and dry shoot weight The application of 50 ppm BA was able to increase the growth and yield of grain compared to without benzyladenine (BA). The combination of biochar and BA enhanced the growth and yield of grain in terms of the number of grains per panicle, dry harvested grain, and dry milled grain. The use of 20 tons/ha biochar combined with 50 ppm BA enhanced the yield of the IR Nutrizinc

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