Phytochemical Screening and Antibacterial Activity of Jackfruit Seed Extract (Artocarpus heterophyllus Lamk.) Against Salmonella typhi In Vitro

Febriza, Ami
Amalia Kartika, Salwa
Asdar

Indonesian Journal of Jamu
Vol. 10 No. 3 (2025): Jurnal Jamu Indonesia

Salmonella typhi infection remains a public health concern, necessitating natural treatment alternatives. This study aimed to evaluate the antibacterial activity and identify the active compounds of jackfruit seed (Artocarpus heterophyllus Lamk) ethanol extract against S. typhi in vitro. Antibacterial activity was tested using the well diffusion method at extract concentrations of 25%, 50%, 75%, and 100%, with chloramphenicol and 10% DMSO as positive and negative controls. All extract concentrations produced significant inhibition zones (mean: 19.83 mm to 31.42 mm; p<0.05). Phytochemical screening revealed flavonoids, alkaloids, steroids, and saponins as the active compounds. Antibacterial efficacy increased with concentration and was comparable to chloramphenicol (mean: 33.49 mm). These results indicate that jackfruit seed extract holds promise as a natural antibacterial agent against S. typhi.