JURNAL PEMULIAAN TANAMAN HUTAN
Vol 9, No 2 (2015): Jurnal pemuliaan Tanaman Hutan

KERAGAMAN GENETIK POPULASI Calophyllum inophyllum MENGGUNAKAN PENANDA RAPD (RANDOM AMPLIFICATION POLYMORPHISM DNA)

ILG Nurtjahjaningsih (Balai Besar Penelitian Bioteknologi dan Pemuliaan Tanaman Hutan)
Titin Haryanti (Fakultas Kehutanan Universitas Gadjah Mada Yogyakarta)
AYPBC Widyatmoko (Balai Besar Penelitian Bioteknologi dan Pemuliaan Tanaman Yogyakarta)
Sapto Indrioko (Fakultas Kehutanan Universitas Gadjah Mada Yogyakarta)
Anto Rimbawanto (Balai Besar Penelitian Bioteknologi dan Pemuliaan Tanaman Hutan Yogyakarta)



Article Info

Publish Date
15 Sep 2015

Abstract

The aims of this study were to assess genetic diversity within populations and genetic relationship among populations of C. inophyllum. Leaf samples as template DNA were collected from 10 natural populations and 1 plantation. Five random amplified polymorphism DNA (RAPD) markers consisted 30 loci were conducted to genetic analysis. Results showed genetic diversity within populations were in low to moderate level (mean HE=0.186). There is no private allele in any populations. The analysis of molecular variance (AMOVA) showed that genetic differentiation among Islands was insignificant; but the differentiation was siginificant among populations and individual trees. Genetic distance among populations was in low to moderate level (mean Da=0.250). Cluster analysis clearly divided the 11 populations into 2 clusters; cluster I consisted Selayar, Lombok, Gunung Kidul and Padang populations; cluster II consisted Way Kambas, Madura, Ketapang, Dompu, and Yapen populations. The genetic relationships did not associate with their geographical locations. In conclusion, genetic diversity and genetic relationship among populations of C. inophyllum was in moderate level.

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