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All Journal JURNAL PEMULIAAN TANAMAN HUTAN
ILG Nurtjahjaningsih
Balai Besar Penelitian Bioteknologi dan Pemuliaan Tanaman Hutan
Agriculture, Biological Sciences & Forestry

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Potensi Populasi Infusi Genetik untuk memperluas variasi genetik kebun benih semai Pinus merkusii di Jember ILG Nurtjahjaningsih
Jurnal Pemuliaan Tanaman Hutan Vol 3, No 2 (2009): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2009.3.2.73-81

Abstract

When genetic base in a seed orchard is narrow, a startegy of genetic infusion should be applied to broaden and maintain its ganetic variation over several successive generations. For this purpose, genetic-infusion populations were established in tree improvement programs of Pinus merkusii located in jember. To be successful genetic variation of the infusion populations should be assessed. This study was aimed to determine genetic diversity of three infusion populations of P merkusii i.e. Takengon, janto and Blankejeren population using five microsatellite markersi. The results  showed that gene diversity of plus trees in the seed orchard was  at a moderate level (HE=0.508).Significant inbreeding deviation from the Hardy-Weinberg equilibrium (HWE) was detected (FIS = 0.073). Gene diversity of the infusion populations was comparatively high. It ranged between 0.498 (Janto) and 0.524 (Blankejeren). The FIS was low and insiqnificant deviation from HWE at any genetic infusion populations. The genetic differentiation among the populations (in both seed orchard and genetic infusion) was very low (FST=0.003) indicating that they were genetically related. It is recommended that the infusion populations have a potential to maintain to genetic diversity in the seed orchard.However, Since they are related, it is necessary to find other potential genetic infusion populations that could broaden genetic diversity more effectively in the seed ochard.
KERAGAMAN GENETIK POPULASI Calophyllum inophyllum MENGGUNAKAN PENANDA RAPD (RANDOM AMPLIFICATION POLYMORPHISM DNA) ILG Nurtjahjaningsih; Titin Haryanti; AYPBC Widyatmoko; Sapto Indrioko; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 9, No 2 (2015): Jurnal pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.852 KB) | DOI: 10.20886/jpth.2015.9.2.103-115

Abstract

The aims of this study were to assess genetic diversity within populations and genetic relationship among populations of C. inophyllum. Leaf samples as template DNA were collected from 10 natural populations and 1 plantation. Five random amplified polymorphism DNA (RAPD) markers consisted 30 loci were conducted to genetic analysis. Results showed genetic diversity within populations were in low to moderate level (mean HE=0.186). There is no private allele in any populations. The analysis of molecular variance (AMOVA) showed that genetic differentiation among Islands was insignificant; but the differentiation was siginificant among populations and individual trees. Genetic distance among populations was in low to moderate level (mean Da=0.250). Cluster analysis clearly divided the 11 populations into 2 clusters; cluster I consisted Selayar, Lombok, Gunung Kidul and Padang populations; cluster II consisted Way Kambas, Madura, Ketapang, Dompu, and Yapen populations. The genetic relationships did not associate with their geographical locations. In conclusion, genetic diversity and genetic relationship among populations of C. inophyllum was in moderate level.
PENGARUH MEDIA DASAR DAN ZAT PENGATUR TUMBUH BAP PADA PERBANYAKAN MIKRO Pinus merkusii ILG Nurtjahjaningsih
Jurnal Pemuliaan Tanaman Hutan Vol 3, No 3 (2009): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2009.3.3.103-116

Abstract

Micropropagation technique is applied to produce large number of plants. Juvenile explants and culture medium are two crucial factors that influence the success.The aims of this study were to determine culture medium that was effective to induce shoot and to produce plantlests from embryo culture of Pinus merkusii. Screening of five cytokines and five basal medium were carried aut. Then, explants wee cultured on the best cytokine and basal medium for plant regeneration. The results showed that BAP was the best cytokine type; LP and GD medium were the best medium compositions for shoots induction. GD medium supplemented by high consentration BAP showed the best medium for shoots induction. Solid GD medium containing 0,25uM BAO was effective for multiple shoots. Liquid GD medium without plant hormone was the best composition for shoots elongation. Half strength of GD medium without plan hormone gave the best result for rooting.
Variasi Genetik Pinus merkusii Menggunakan Penanda Mikrosatelit Kloroplas ILG Nurtjahjaningsih; AYPBC Widyatmoko; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 5, No 3 (2011): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2011.5.3.119-128

Abstract

Using two chloroplast universal primers, haplotipic within and among populations of Pinus merkusii had been evaluated, in order to access paternal variation of this species. The aim of this study was to investigate variation of chloroplast microsatellite in P. merkusii. Using two universal chloroplast microsatelites, a total of 18 chloroplast haplotypes were found among 730 individuals surveyed in 10populations in Java. Private haplotypes mostly found at eastern Java populations. The number ofdetected haplotypes (Na) within populations ranged between 5 and 14. Variation of haplotypes (h) was relatively hight; it ranged between 0.727 and 0.858. Nei’s genetic distance among populations was very low (Da=0.030). Moreover, analysis of molecular variance (AMOVA) showed genetic variation originated from within population but the value was insignificant.It was concluded that private haplotypes found in eastern Java might show gene pool of P. merkusii in Java. These detected haplotypes are of important information for genetic resources conservation anf breeding strategy of P. merkusii.
Co-Authors Anto Rimbawanto Anto Rimbawanto AYPBC Widyatmoko AYPBC Widyatmoko Sapto Indrioko Titin Haryanti